Construct: ORF TRCN0000479395
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000152.1_s317c1
- Derived from:
- ccsbBroadEn_05725
- DNA Barcode:
- GTAGGCACGAAATGGCCCTAATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM153CP (653316)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479395
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285596 | FAM153A | family with sequence simila... | XM_011534528.2 | 44.3% | 43.9% | (many diffs) |
| 2 | human | 202134 | FAM153B | family with sequence simila... | NM_001265615.1 | 36.5% | 36.1% | (many diffs) |
| 3 | human | 285596 | FAM153A | family with sequence simila... | NM_173663.4 | 36.4% | 36.1% | (many diffs) |
| 4 | human | 285596 | FAM153A | family with sequence simila... | XM_006714848.3 | 36.4% | 36.1% | (many diffs) |
| 5 | human | 285596 | FAM153A | family with sequence simila... | XM_006714849.3 | 36.4% | 36.1% | (many diffs) |
| 6 | human | 285596 | FAM153A | family with sequence simila... | XM_011534521.3 | 36.4% | 36.1% | (many diffs) |
| 7 | human | 285596 | FAM153A | family with sequence simila... | XM_011534522.2 | 36.4% | 36.1% | (many diffs) |
| 8 | human | 285596 | FAM153A | family with sequence simila... | XM_011534523.2 | 36.4% | 36.1% | (many diffs) |
| 9 | human | 285596 | FAM153A | family with sequence simila... | XM_017009366.1 | 36.4% | 36.1% | (many diffs) |
| 10 | human | 285596 | FAM153A | family with sequence simila... | XM_017009367.1 | 36.4% | 36.1% | (many diffs) |
| 11 | human | 285596 | FAM153A | family with sequence simila... | XM_017009369.1 | 36.4% | 36.1% | (many diffs) |
| 12 | human | 285596 | FAM153A | family with sequence simila... | XM_017009370.1 | 36.4% | 36.1% | (many diffs) |
| 13 | human | 285596 | FAM153A | family with sequence simila... | XM_017009365.1 | 34% | 33.7% | (many diffs) |
| 14 | human | 285596 | FAM153A | family with sequence simila... | XM_017009363.1 | 33.8% | 33.5% | (many diffs) |
| 15 | human | 285596 | FAM153A | family with sequence simila... | XM_017009364.1 | 33.8% | 33.5% | (many diffs) |
| 16 | human | 285596 | FAM153A | family with sequence simila... | XM_017009362.1 | 33.3% | 33% | (many diffs) |
| 17 | human | 285596 | FAM153A | family with sequence simila... | XM_011534520.2 | 29.3% | 29% | (many diffs) |
| 18 | human | 285596 | FAM153A | family with sequence simila... | XM_005265889.3 | 29.1% | 28.9% | (many diffs) |
| 19 | human | 285596 | FAM153A | family with sequence simila... | XM_017009352.1 | 29.1% | 28.9% | (many diffs) |
| 20 | human | 285596 | FAM153A | family with sequence simila... | XM_017009353.1 | 29.1% | 28.9% | (many diffs) |
| 21 | human | 285596 | FAM153A | family with sequence simila... | XM_017009354.1 | 29.1% | 28.9% | (many diffs) |
| 22 | human | 285596 | FAM153A | family with sequence simila... | XM_017009355.1 | 29.1% | 28.9% | (many diffs) |
| 23 | human | 285596 | FAM153A | family with sequence simila... | XM_017009356.1 | 29.1% | 28.9% | (many diffs) |
| 24 | human | 285596 | FAM153A | family with sequence simila... | XM_017009357.1 | 29.1% | 28.9% | (many diffs) |
| 25 | human | 285596 | FAM153A | family with sequence simila... | XM_017009358.1 | 29.1% | 28.9% | (many diffs) |
| 26 | human | 285596 | FAM153A | family with sequence simila... | XM_017009359.1 | 29.1% | 28.9% | (many diffs) |
| 27 | human | 285596 | FAM153A | family with sequence simila... | XM_017009360.1 | 29.1% | 28.9% | (many diffs) |
| 28 | human | 285596 | FAM153A | family with sequence simila... | XM_017009361.1 | 29.1% | 28.9% | (many diffs) |
| 29 | human | 653316 | FAM153CP | family with sequence simila... | NR_159407.1 | 28.2% | 1_241del;584_1210del | |
| 30 | human | 653316 | FAM153CP | family with sequence simila... | NR_149722.1 | 13.4% | 1_555del;898_2552del | |
| 31 | human | 285596 | FAM153A | family with sequence simila... | NR_146227.1 | 7.2% | (many diffs) | |
| 32 | human | 285596 | FAM153A | family with sequence simila... | NR_146226.1 | 7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 411
- ORF length:
- 342
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtggacaaa gacacagaga gagacattga gatgaaacgg caacTACGGC 121 GACTACGGGA GCTCCACCTA TACAGCACAT GGAAGAAGTA CCAAGAGGCG ATGAAGACAT 181 CCTTGGGAGT TCCACAACGT GGTGACCTGG AAGACCTGGA GGAGCATCTG CCAGGGCAGA 241 CAGTCTCTGA GGAAGCCACA GGGGTTCACA TGATGCAGGT GGACCCAGCC ACACCGGCAA 301 AGAAATTGGA AGACTCCACC ATTACAGGCA GCCACCAGCA GATGTCAGCA AGTCCTTCCT 361 CTGCACCTGC AGAAGAAGCA ACAGAAAAGA CCAAAGTGGA AGAGGAAGTG TTGCCAACTT 421 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 481 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 541 CTTGTGGAAA GGACGAGTAG GCACGAAATG GCCCTAATGA ACGCGTTAAG TCgacaatca 601 acctctggat tacaaaattt gtgaaagatt