Construct: ORF TRCN0000479412
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008517.1_s317c1
- Derived from:
- ccsbBroadEn_05647
- DNA Barcode:
- TATCCTGTCCGACTTTCCCACACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOMM5 (401505)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479412
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401505 | TOMM5 | translocase of outer mitoch... | NM_001001790.3 | 100% | 100% | |
| 2 | human | 401505 | TOMM5 | translocase of outer mitoch... | NM_001134485.1 | 60% | 58.8% | 122_223del |
| 3 | human | 401505 | TOMM5 | translocase of outer mitoch... | NM_001134484.1 | 50.3% | 40.4% | (many diffs) |
| 4 | human | 107986372 | LOC107986372 | mitochondrial import recept... | XR_002956250.1 | 23.3% | (many diffs) | |
| 5 | human | 107986373 | LOC107986373 | mitochondrial import recept... | XR_002956251.1 | 23.3% | (many diffs) | |
| 6 | mouse | 68512 | Tomm5 | translocase of outer mitoch... | NM_001099675.1 | 90.1% | 96% | (many diffs) |
| 7 | mouse | 68512 | Tomm5 | translocase of outer mitoch... | NM_001134646.1 | 69.4% | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 173
- ORF end:
- 326
- ORF length:
- 153
- Sequence:
-
1 GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT 61 GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG 121 GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGTTCCG 181 GATTGAGGGC CTCGCGCCGA AGCTGGACCC GGAGGAGATG AAACGGAAGA TGCGCGAGGA 241 TGTGATCTCC TCCATACGGA ACTTTCTCAT CTACGTGGCC CTCCTGCGAG TCACTCCATT 301 TATCTTAAAG AAATTGGACA GCATATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 361 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 421 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ATATCCTGTC 481 CGACTTTCCC ACACAACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 541 agatt