Construct: ORF TRCN0000479425
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005283.1_s317c1
- Derived from:
- ccsbBroadEn_06701
- DNA Barcode:
- ACCAATCCGTGTGAATCCACAGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE3A (5139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479425
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5139 | PDE3A | phosphodiesterase 3A | NM_000921.4 | 99.9% | 100% | 1194C>T;2172C>G |
| 2 | human | 5139 | PDE3A | phosphodiesterase 3A | XM_017019420.1 | 94.2% | 92% | (many diffs) |
| 3 | human | 5139 | PDE3A | phosphodiesterase 3A | XM_017019421.2 | 71.8% | 71.8% | 0_1ins963;231C>T;1209C>G |
| 4 | human | 5139 | PDE3A | phosphodiesterase 3A | NM_001244683.1 | 70.7% | 70.4% | (many diffs) |
| 5 | mouse | 54611 | Pde3a | phosphodiesterase 3A, cGMP ... | XM_011241595.2 | 61.6% | 62.9% | (many diffs) |
| 6 | mouse | 54611 | Pde3a | phosphodiesterase 3A, cGMP ... | XM_006506979.3 | 61.6% | 63% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3492
- ORF length:
- 3423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagtgccc ggcgacgctg cacgagtcag ggacaagccc gtccacagtg 121 gggtgagtca agcccccacg gcgggccggg actgccacca tcgtgcggac cccgcatcgc 181 cgcgggactc gggctgccgt ggctgctggg gagacctggt gctgcagccg ctccggagct 241 ctcggaaact ttcctccgcg ctgtgcgcgg gctccctgtc ctttctgctg gcgctgctgg 301 tgaggctggt ccgcggggag gtcggctgtg acctggagca gtgtaaggag gcggcggcgg 361 cggaggagga ggaagcagcc ccgggagcag aagggggcgt cttcccgggg cctcggggag 421 gtgctcccgg gggcggtgcg cggctcagcc cctggctgca gccctcggcg ctgctcttca 481 gtctcctgtg tgccttcttc tggatgggct tgtacctcct gcgcgccggg gtgcgcctgc 541 ctctggctgt cgcgctgctg gccgcctgct gcggggggga agcgctcgtc cagattgggc 601 tgggcgtcgg ggaggatcac ttactctcac tccccgccgc gggggtggtg ctcagctgct 661 tggccgccgc gacatggctg gtgctgaggc tgaggctggg cgtcctcatg atcgccttga 721 ctagcgcggt caggaccgtg tccctcattt ccttagagag gttcaaggtc gcctggagac 781 cttacctggc gtacctggcc ggcgtgctgg ggatcctctt ggccaggtac gtggaacaaa 841 tcttgccgca gtccgcggag gcggctccaa gggagcattt ggggtcccag ctgattgctg 901 ggaccaagga agatatcccg gtgtttaaga ggaggaggcg gtccagctcc gtcgtgtccg 961 ccgagatgtc cggctgcagc agcaagtccc atcggaggac ctccctgccc tgtataccga 1021 gggaacagct catggggcat tcagaatggg accacaaacg agggccaaga ggatcacagt 1081 cttcaggaac cagtattact gtggacatcg ccgtcatggg cgaggcccac ggcctcatta 1141 ccgacctcct ggcagaccct tctcttccac caaacgtgtg cacatccttg agagccgtga 1201 gcaacttgct cagcacacag ctcaccttcc aggccattca caagcccaga gtgaatcccg 1261 ttacttcgct cagtgaaaac tatacctgtt ctgactctga agagagctct gaaaaagaca 1321 agcttgctat tccaaagcgc ctgagaagga gtttgcctcc tggcttgttg agacgagttt 1381 cttccacttg gaccaccacc acctcggcca caggtctacc caccttggag cctgcaccag 1441 tacggagaga ccgcagcacc agcatcaaac tgcaggaagc accttcatcc agtcctgatt 1501 cttggaataa tccagtgatg atgaccctca ccaaaagcag atcctttact tcatcctatg 1561 ctatttctgc agctaaccat gtaaaggcta aaaagcaaag tcgaccaggt gccctcgcta 1621 aaatttcacc tctttcatcg ccctgctcct cacctctcca agggactcct gccagcagcc 1681 tggtcagcaa aatttctgca gtgcagtttc cagaatctgc tgacacaact gccaaacaaa 1741 gcctaggttc tcacagggcc ttaacttaca ctcagagtgc cccagaccta tcccctcaaa 1801 tcctgactcc acctgttata tgtagcagct gtggcagacc atattcccaa gggaatcctg 1861 ctgatgagcc cctggagaga agtggggtag ccactcggac accaagtaga acagatgaca 1921 ctgctcaagt tacctctgat tatgaaacca ataacaacag tgacagcagt gacattgtac 1981 agaatgaaga tgaaacagag tgcctgagag agcctctgag gaaagcatcg gcttgcagca 2041 cctatgctcc tgagaccatg atgtttctgg acaaaccaat tcttgctccc gaacctcttg 2101 tcatggataa cctggactca attatggagc agctaaatac ttggaatttt ccaatttttg 2161 atttagtgga aaatatagga agaaaatgtg gccgtattct tagtcaggta tcttacagac 2221 tttttgaaga catgggcctg tttgaagctt ttaaaattcc aattagggaa tttatgaatt 2281 attttcatgc tttggagatt ggatataggg atattcctta tcataacaga atccatgcca 2341 ctgatgtttt acatgctgtt tggtatctta ctacacagcc tattccaggc ctctcaactg 2401 tgattaatga tcatggttca accagtgatt cagattctga cagtggattt acacatggac 2461 atatgggata tgtattctca aaaacgtata atgtgacaga tgataaatac ggatgtctgt 2521 ctgggaatat ccctgccttg gagttgatgg cgctgtatgt ggctgcagcc atgcacgatt 2581 atgatcatcc aggaaggact aatgctttcc tggttgcaac tagtgctcct caggcggtgc 2641 tatataacga tcgttcagtt ttggagaatc atcacgcagc tgctgcatgg aatcttttca 2701 tgtcccggcc agagtataac ttcttaatta accttgacca tgtggaattt aagcatttcc 2761 gtttccttgt cattgaagca attttggcca ctgacctgaa gaaacacttt gacttcgtag 2821 ccaaatttaa tggcaaggta aatgatgatg ttggaataga ttggaccaat gaaaatgatc 2881 gtctactggt ttgtcaaatg tgtataaagt tggctgatat caatggtcca gctaaatgta 2941 aagaactcca tcttcagtgg acagatggta ttgtcaatga attttatgaa cagggtgatg 3001 aagaggccag ccttggatta cccataagcc ccttcatgga tcgttctgct cctcagctgg 3061 ccaaccttca ggaatccttc atctctcaca ttgtggggcc tctgtgcaac tcctatgatt 3121 cagcaggact aatgcctgga aaatgggtgg aagacagcga tgagtcagga gatactgatg 3181 acccagaaga agaggaggaa gaagcaccag caccaaatga agaggaaacc tgtgaaaata 3241 atgaaTCTCC AAAAAAGAAG ACTTTCAAAA GGAGAAAAAT CTACTGCCAA ATAACTCAGC 3301 ACCTCTTACA GAACCACAAG ATGTGGAAGA AAGTCATTGA AGAGGAGCAA CGGTTGGCAG 3361 GCATAGAAAA TCAATCCCTG GACCAGACCC CTCAGTCGCA CTCTTCAGAA CAGATCCAGG 3421 CTATCAAGGA AGAAGAAGAA GAGAAAGGGA AACCAAGAGG CGAGGAGATA CCAACCCAAA 3481 AGCCAGACCA GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACC AATCCGTGTG AATCCACAGT 3661 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t