Construct: ORF TRCN0000479434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003875.1_s317c1
- Derived from:
- ccsbBroadEn_10589
- DNA Barcode:
- TTCTGCTTCAGCTGTCCAGGAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT8P41 (283102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479434
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3856 | KRT8 | keratin 8 | NM_001256293.2 | 36.5% | 31.6% | (many diffs) |
| 2 | human | 3856 | KRT8 | keratin 8 | NM_002273.4 | 36.5% | 31.6% | (many diffs) |
| 3 | human | 3856 | KRT8 | keratin 8 | NM_001256282.2 | 34.5% | 29.8% | (many diffs) |
| 4 | human | 283102 | KRT8P41 | keratin 8 pseudogene 41 | NR_027713.1 | 32.3% | 1_732del;1324_1828del | |
| 5 | human | 3856 | KRT8 | keratin 8 | NR_045962.2 | 24.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 660
- ORF length:
- 591
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacaaggta gagctggagt ctcttctgga agggctgact gacgagatca 121 acttcctcag gcagctatat gaagaggaaa tctgggagct gcagtcccag atctcggaca 181 catctgtggt gctgtccatg gacagcagcc gctccctgga catggacagc atcatccctg 241 aggtcaaggc gcagtacaag gagatcgcca atggcagctg ggctgaggct gagggcatgc 301 atcagatcaa gtatgaggag ctgcagacac tgcctgggaa gcataggaat gacctgcgtt 361 atgcaaagat ggagatctct gagattaata ggaacatcag ccggctCCAG GCTCAGACTG 421 AGGGCCTCAA AGGCCAGAGG GTTTCCCTGG AGGCTGCCAT CGCAGATGCT GAGCAGTATG 481 GGGAGCTGGC TGTTAAGGAT GCCAACACGA AGCTGTCGAG CTGGAGGCCG CCCTGCAGCG 541 GGCCGAGCAA GACATGGTGC AGCAGCTGTG CCATGGAGCA CCAGGAGCTG ATGAACGTCA 601 AGCTGGCCCT GGACATCAAG ATCGCCACCT ACAGGAAGCT GCTGGAGGGC GAGGAGAGCT 661 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGATTCTG CTTCAGCTGT CCAGGAGGTA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt