Transcript: Human NM_002273.4

Homo sapiens keratin 8 (KRT8), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
KRT8 (3856)
Length:
1784
CDS:
96..1547

Additional Resources:

NCBI RefSeq record:
NM_002273.4
NBCI Gene record:
KRT8 (3856)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002273.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000421337 GGATGCAGAACATGAGTATTC pLKO_005 1309 CDS 100% 10.800 7.560 N KRT8 n/a
2 TRCN0000062385 TCGAAGCAACATGGACAACAT pLKO.1 491 CDS 100% 4.950 3.465 N KRT8 n/a
3 TRCN0000422059 AGGCACAGTACGAGGATATTG pLKO_005 886 CDS 100% 13.200 7.920 N KRT8 n/a
4 TRCN0000062386 GCAGCTATATGAAGAGGAGAT pLKO.1 770 CDS 100% 4.050 2.430 N KRT8 n/a
5 TRCN0000062384 GCCTCCTTCATAGACAAGGTA pLKO.1 402 CDS 100% 3.000 1.800 N KRT8 n/a
6 TRCN0000062383 CTGGACATGGACAGCATCATT pLKO.1 855 CDS 100% 5.625 2.813 Y KRT8 n/a
7 TRCN0000116955 GAGGACTTCAAGAACAAGTAT pLKO.1 612 CDS 100% 5.625 2.813 Y KRT8P11 n/a
8 TRCN0000084030 CAACAAGTTTGCCTCCTTCAT pLKO.1 392 CDS 100% 4.950 2.475 Y KRT6A n/a
9 TRCN0000062387 GCAGATCAAGACCCTCAACAA pLKO.1 374 CDS 100% 4.950 2.475 Y KRT8 n/a
10 TRCN0000082891 CAACAACAAGTTTGCCTCCTT pLKO.1 389 CDS 100% 2.640 1.320 Y KRT6B n/a
11 TRCN0000305998 AGCGTACAGAGATGGAGAATG pLKO_005 649 CDS 100% 10.800 5.400 Y Krt8 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002273.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10940 pDONR223 100% 57.7% 57.7% None 1_612del n/a
2 ccsbBroad304_10940 pLX_304 0% 57.7% 57.7% V5 1_612del n/a
3 TRCN0000467772 ACAAATATGTCAAGAGTTCTGACC pLX_317 14.7% 57.7% 57.7% V5 1_612del n/a
4 ccsbBroadEn_10589 pDONR223 100% 36.5% 31.6% None (many diffs) n/a
5 ccsbBroad304_10589 pLX_304 0% 36.5% 31.6% V5 (many diffs) n/a
6 TRCN0000479434 TTCTGCTTCAGCTGTCCAGGAGGT pLX_317 42.9% 36.5% 31.6% V5 (many diffs) n/a
7 ccsbBroadEn_12931 pDONR223 100% 22.2% 17.5% None (many diffs) n/a
8 ccsbBroad304_12931 pLX_304 0% 22.2% 17.5% V5 (many diffs) n/a
9 TRCN0000471683 GTTCGCATTGACTCGACCACCATG pLX_317 100% 22.2% 17.5% V5 (many diffs) n/a
Download CSV