Construct: ORF TRCN0000479436
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015190.1_s317c1
- Derived from:
- ccsbBroadEn_09021
- DNA Barcode:
- TGAAGCACCCCTAAAAATAATAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CXorf21 (80231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479436
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80231 | CXorf21 | chromosome X open reading f... | NM_025159.3 | 99.8% | 100% | 627T>G |
| 2 | mouse | 71398 | 5430427O19Rik | RIKEN cDNA 5430427O19 gene | NM_001163539.1 | 85% | 83.3% | (many diffs) |
| 3 | mouse | 71398 | 5430427O19Rik | RIKEN cDNA 5430427O19 gene | XM_011247682.2 | 85% | 83.3% | (many diffs) |
| 4 | mouse | 71398 | 5430427O19Rik | RIKEN cDNA 5430427O19 gene | XM_017318620.1 | 85% | 83.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 969
- ORF length:
- 903
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtcagaaggg tatctcagtg gacttgagta ctggaatgac atccactgga 121 gttgtgcctc ttataatgag caggtggctg gggaaaagga agaggagaca aattctgttg 181 ctaccctttc ctattcctct gtggatgaaa cacaagtcag aagtctctac gtgagctgca 241 aatcatctgg caagtttatc tcttcagtgc attcaagaga gagccaacat agcagaagtc 301 agagagtcac agtgctgcag acaaacccca atcctgtgtt tgaaagccca aacttggctg 361 cagttgaaat atgtagagat gccagcagag agacctactt ggttccatct tcttgcaaaa 421 gtatttgcaa gaattataat gacttacaga ttgcaggggg ccaggtgatg gccattaatt 481 cagtgacaac agattttccc tctgagagca gttttgaata tggccctttg ctgaagtcat 541 ctgagattcc tttacccatg gaggattcca tttctactca gcccagtgac tttccccaaa 601 aacctatcca gcggtactca tcctattgga gaataacaag catcaaagag aaaagcagct 661 tgcaaatgca gaatcctatt tctaatgcag tgctgaatga gtacctggag cagaaggttg 721 tggagttata taaacagtac attatggaca ccgtgtttca tgacagttct cctacccaga 781 ttctggcgtc tgaactcatc atgacaagtg tagaccaaat cagtcttcaa gtgtctagag 841 agaagaatct ggagaCCTCA AAAGCCAGAG ATATAGTCTT TAGCCGCCTA TTGCAATTGA 901 TGTCAACTGA AATTACTGAA ATTAGCACTC CTAGTCTCCA TATTTCTCAG TATAGCAATG 961 TAAATCCATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1021 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1081 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGAAGC ACCCCTAAAA ATAATAAAAC 1141 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt