Construct: ORF TRCN0000479437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014616.1_s317c1
- Derived from:
- ccsbBroadEn_11932
- DNA Barcode:
- TTCCAAGGGCTAGGCTGATAGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VILL (50853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479437
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50853 | VILL | villin like | NM_001370264.1 | 97.9% | 97.9% | 274_315del |
| 2 | human | 50853 | VILL | villin like | NM_001370265.1 | 96% | 93.1% | (many diffs) |
| 3 | human | 50853 | VILL | villin like | NM_015873.3 | 78.5% | 78.5% | 1_510del;784_825del |
| 4 | human | 50853 | VILL | villin like | NR_163266.1 | 74.3% | (many diffs) | |
| 5 | human | 50853 | VILL | villin like | NR_163267.1 | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2082
- ORF length:
- 2016
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gattcagtgg aatgggccca agaccagcat ttctgagaag gctcgggggc 121 tggctttgac ctacagcctc cgggacaggg aacgtggtgg tggtcgtgca cagattggtg 181 tggtggatga tgaggccaaa gccccggacc tcatgcagat catggaggct gtgctgggcc 241 gcagggtggg cagcctgcgt gccgccacgc ccagcaagga tatcaaccag ctgcagaagg 301 ccaatgttcg cctgtaccat gtctatgaga agggcaaaga cctgctgcag gaggaggact 361 tctacatcct ggaccagggt ggcttcaaga tctatgtgtg gcagggacgc atgtctagcc 421 tccaggagag aaaggctgcc ttcagccggg ctgtgggctt catccaggcc aagggctacc 481 cgacctacac caacgtggag gtggtgaacg acggcgccga gtcggccgcg ttcaagcagc 541 tcttccggac ttggtctgag aagcggcgca ggaaccagaa gctcggcggg agggataaat 601 cgattcatgt aaagctggac gtgggcaagc tgcacaccca gcctaagtta gcggcccagc 661 tcaggatggt ggacgacggc tctgggaagg tggaggtgtg gtgcatccag gacttacaca 721 ggcagcccgt ggaccccaag cgtcatggac agctgtgtgc aggcaactgc taccttgtgc 781 tctacacata ccagaggctg ggccgtgtcc agtacatcct gtacctatgg cagggccacc 841 aggccactgc ggatgagatt gaggccctga acagcaacgc tgaggaacta gatgtcatgt 901 atggtggcgt cctagtacag gagcatgtga ccatgggcag cgagcccccc cacttcctcg 961 ccatcttcca gggccagctg gtgatcttcc aggagagagc tgggcaccat ggaaaggggc 1021 agtcagcatc caccacaagg cttttccaag tgcaaggcac tgacagccac aacaccagga 1081 ccatggaggt gccagcccgt gcctcatccc tcaactccag tgacatcttc ttgctggtca 1141 cagccagcgt ctgctacctc tggtttggga agggctgtaa tggtgatcag cgtgagatgg 1201 cacgggtggt ggtcactgtc atttccagga agaatgagga aacggtgctg gagggtcagg 1261 agcctcccca cttctgggag gccctgggag gccgggcccc ctaccccagc aacaagaggc 1321 tccctgagga ggtccccagc ttccagccac gactgtttga gtgctccagc cacatgggct 1381 gcctggtcct cgcagaagtg gggttcttca gccaggagga cctggacaag tatgacatca 1441 tgttactgga cacctggcag gagatcttcc tgtggcttgg ggaagctgca agtgagtgga 1501 aggaggcggt ggcctggggc caggagtacc tgaagactca cccagcaggg aggagcccgg 1561 ccacacccat cgtgctggtc aagcagggcc atgagcctcc caccttcatt ggatggttct 1621 tcacttggga cccctacaag tggactagcc acccgtccca caaggaagtg gtggatggca 1681 gcccggcagc agcatcaacc atctcTGAGA TAACAGCAGA AGTCAACAAC TTGCGGCTAT 1741 CCAGATGGCC GGGCAATGGC AGGGCAGGTG CCGTGGCCCT GCAGGCCCTC AAGGGCTCCC 1801 AGGACAGCTC AGAGAATGAT CTGGTGCGAA GCCCCAAGTC GGCTGGCAGC AGAACCAGCA 1861 GCTCCGTCAG CAGCACCAGC GCCACGATCA ACGGGGGCCT GCGCCGGGAA CAACTGATGC 1921 ACCAGGCTGT TGAGGACCTG CCAGAGGGCG TGGACCCTGC CCGCAGGGAG TTCTATCTCT 1981 CAGACTCTGA CTTCCAAGAT ATCTTTGGGA AATCCAAGGA GGAATTCTAC AGCATGGCCA 2041 CGTGGAGGCA GCGGCAGGAG AAAAAGCAGC TGGGCTTCTT CTGCCCAACT TTCTTGTACA 2101 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2161 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2221 AGGACGATTC CAAGGGCTAG GCTGATAGGC CACGCGTTAA GTCgacaatc aacctctgga 2281 ttacaaaatt tgtgaaagat t