Construct: ORF TRCN0000479449

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002892.1_s317c1
Derived from:
ccsbBroadEn_12834
DNA Barcode:
GCCCCTGTTACTTCTATGTGCCCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FNDC1 (84624)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479449
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84624 FNDC1 fibronectin type III domain... NM_032532.3 93.3% 93.2% (many diffs)
2 human 84624 FNDC1 fibronectin type III domain... XM_011536190.2 92.1% 92% (many diffs)
3 human 84624 FNDC1 fibronectin type III domain... XM_011536191.2 91.1% 90.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
5388
ORF length:
5319
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggcctgaaa gtcacgtggg acccacccaa agatgctacc agtagacctg
121tggagcatta caacattgcc tatgggaagt cactgaaaag tcttaaatac atcaaggtga
181atgcggagac atactccttc cttattgagg atgtggagcc gggggtagtg tactttgtgc
241tgcttactgc agaaaaccac agtggagtga gccgtcctgt ttacagagct gaaagcccac
301ctggaggtga atggatcgag attgatggtt ttcccattaa gggtccagga ccatttaatg
361aaaccgtcac agaaaaggaa gtgcccaaca agcccttgcg tgtgcgtgtc cggtcctcag
421atgacaggct gtccgttgcg tggaaggcac cacgcctgtc tggagccaag agtccacgca
481gatcacgggg ttttctcctg ggctacgggg agagtggccg gaagatgaat tatgttccac
541tgacaagaga tgaacggaca cacgaaatta aaaagctagc ctcggaatcc gtgtatgtgg
601tctccctgca gtccatgaac tctcagggcc ggagccaacc agtctacagg gctgccctaa
661caaagcgaaa gatttcagaa gaggacgaat tggatgtacc tgacgacatc agcgtccggg
721ttatgtcatc tcagtctgtg cttgtgtcct gggtggatcc tgttctggaa aaacagaaga
781aagttgttgc atcaagacag tacaccgtgc gctatcgaga gaagggggaa ttggccaggt
841gggattataa gcagatcgct aacaggcgtg tgctgattga gaacctgatt ccagacactg
901tgtatgaatt tgcagtccgt atttcacagg gtgaaagaga tggcaaatgg agtacgtcag
961tcttccaaag aacaccagaa tctgccccta ccacagctcc tgaaaacttg aacgtctggc
1021cagtcaatgg caaacctaca gttgtcgctg catcttggga tgcgctacca gagactgagg
1081ggaaagtgaa agccagtaag gcggatgttc agcagaacac ggaggacaat gggaaacccg
1141aaaaacctga gccttcctca ccttctccca gagctccagc ttcctcccaa cacccctctg
1201tgcctgcttc tccccaaggg agaaatgcca aggaccttct tcttgacttg aagaacaaaa
1261tattggctaa tggtggggcg ccccgaaaac cccagcttcg cgccaagaag gcagaggagc
1321tggatcttca gtcgacagaa atcactgggg aggaggagct gggttcccgg gaggactcgc
1381ccatgtcacc ctcagacacc caagaccaga aacggaccct gaggccgcca agtagacacg
1441gccactcggt ggttgctccc ggcaggactg cagtgagggc ccggatgcca gcgctgcccc
1501gaagggaagg cgtagataag cctggctttt ccctggccac gcagccccgc ccaggggcgc
1561ccccctcggc ttcggcctct cctgcccacc acgcgtccac ccagggcacc tctcatcgtc
1621cttccctgcc tgccagcttg aatgacaacg acttggtgga ctcagacgaa gatgagcgcg
1681ctgtgggctc cctccacccc aagggcgcct tcgcccagcc ccggccagcc ctgtccccca
1741gccgccagtc cccgtccagc gttctccgcg acagaagctc tgtgcacccc ggcgcaaagc
1801cagcctcgcc ggcccggagg accccccatt caggggccgc agaggaagat tccagtgcct
1861cagccccacc ctcaagactt tctccacccc atgggggatc atctcggctg ctgcccaccc
1921agccacacct gagctctcca ctttccaagg gcgggaagga tggtgaggac gccccagcca
1981ccaactccaa tgcgccatca cggtccacca tgtcctcctc cgtctcttct catctctcgt
2041ccaggacgca ggtctctgag ggagcggagg cttctgatgg tgaaagccac ggtgacggcg
2101atagggaaga cggcggaagg caggcggagg ccacggccca gacgctgcgg gcccggcctg
2161cctctggaca cttccatttg ctcagacaca aaccctttgc tgccaacggg aggtctccaa
2221gcaggttcag cattgggcgg ggacctcggc tgcagccctc cagctcccca cagtcgactg
2281tgccctcccg agcccacccc agggttccct ctcactctga ttcccaccct aagcttagct
2341caggtatcca tggagacgag gaggatgaga agccgcttcc tgccaccgtt gtcaatgacc
2401acgtgccttc ctcctccagg cagcccatct cccggggctg ggaggactta aggagaagcc
2461cgcagagagg ggccagcctg catcggaagg aacccatccc agagaacccc aaatccacag
2521gggcagatac acatcctcag ggcaagtact cctccctggc ctccaaggct caggatgttc
2581aacagagcac agacgcggac acggagggtc attctcccaa agcacagcca gggtccacag
2641accgccacgc gtcccctgct cgtcctcccg cagcacggtc acagcagcat cccagtgttc
2701ccagaaggat gacacccggc cgggccccag aacagcagcc ccctcctccc gtcgccacgt
2761cccagcacca cccgggaccc cagagcagag acgcgggtcg gtcaccttcc cagcccaggc
2821tctcactgac ccaggccggg cggccccgcc ccacgtcgca gggccgctcc cactcctcct
2881cggaccctta cacggcgagc tccagaggga tgctccccac ggccctccag aaccaggacg
2941aggatgccca gggcagctac gacgacgaca gcacagaagt cgaggcccag gatgtgcggg
3001cccccgcaca cgccgcgcgc gccaaggagg cagctgcgtc ccttcccaag caccagcagg
3061tggagtctcc cacaggcgca ggggcaggtg gtgaccacag gtcccagcgc ggacatgcgg
3121cctcccccgc caggcccagc cgacccggcg gcccccagtc ccgcgcccgg gtccccagca
3181gggcagcgcc ggggaagtcg gagcctcctt ccaagcggcc cctgtcctcc aagtcccagc
3241agtcggtctc agccgaggac gaggaggagg aggacgcggg gttttttaaa ggcgggaaag
3301aagaccttct gtcttcctct gtgccaaagt ggccctcttc ctccactccc aggggcggca
3361aagacgccga tgggagcctc gccaaggaag agagggagcc tgccatcgcg cttgcccctc
3421gcggagggag cctggctcct gtgaagcgac ctctcccccc acctccaggc agctccccca
3481gggcctccca cgtcccttcc cgaccgccgc ctcgcagcgc tgccaccgtg agccccgtcg
3541cgggcaccca cccctggccg cggtacacca cgcgcgcccc gcctggccac ttctccacca
3601ccccgatgct gtccttgcgc cagaggatga tgcatgccag attccgtaac cctctctccc
3661gacagcctgc cagaccctct tacagacaag gttataatgg cagaccaaat gtagaaggga
3721aagtccttcc tggtagtaat ggaaaaccga atggacagag aattatcaat ggccctcaag
3781gaacaaagtg ggttgtggac cttgatcgtg ggttagtatt gaatgcagaa ggaaggtacc
3841tccaagattc acatggaaat cctcttcgga ttaaactagg aggagatggt cgaaccattg
3901tagatctgga agggaccccc gtggtgagtc ctgacggcct cccactcttt gggcaggggc
3961gacatggcac acctctggcc aatgcccaag ataagccaat tttgagtctt ggaggaaagc
4021cgctggtggg tttggaggtc atcaaaaaaa ccacccatcc ccctaccact accatgcagc
4081ccaccactac tacgacgccc ctgcctacca ctacaacccc gaggcccacc actgccacca
4141cccgccgcac gaccaccagg cgtccaacaa ccacagtccg aaccactacg cggacaacca
4201ccaccaccac ccccaaaccc accactccca tccccacctg tccccctggg accttggaac
4261ggcacgacga tgatggcaac ctgataatga gctccaatgg gatcccagag tgctacgctg
4321aagaagatga gttctcaggc ttggagactg acactgcagt acctacggaa gaggcctacg
4381ttatatatga tgaagattat gaatttgaga cgtcaaggcc accaaccacc actgagcctt
4441cgaccactgc taccacaccg agggtgatcc cagaggaagg cgccatcagt tcctttcctg
4501aagaagaatt tgatctggct ggaaggaaac gatttgttgc tccttacgtg acgtacctaa
4561ataaagaccc atcagccccg tgctctctga ctgatgcact ggatcacttc caagtggaca
4621gcctcgatga aatcatcccc aatgacctga agaagagtga cctgcctccc cagcatgctc
4681cccgcaacat caccgtggtg gccgtggaag gttgccactc atttgtcatt gtggactggg
4741acaaagctac cccaggagat gtggtcacag gttacttggt ttacagtgca tcctatgaag
4801acttcatcag gaacaagtgg tccactcaag cttcatcagt aactcacttg cccattgaga
4861acctaaagcc caacacgagg tattatttta aagtgcaagc acaaaatcct catggctacg
4921gacctatcag cccttcggtc tcatttgtca ccgaatcaga taatcctctg cttgttgtga
4981ggcccccagg cggtgagcct atctggatcc cattcgcttt caaacatgat cccagctaca
5041cggactgcca tggacggcaa tatgtgaagc gcacgtggta tcgaaagttc gtgggagttg
5101ttctttgtaa ttcactgagg tataaaatct acctcagtga caacctgaaa gatacaTTCT
5161ACAGCATTGG AGACAGCTGG GGAAGAGGTG AAGACCATTG CCAATTTGTG GATTCACACC
5221TTGATGGAAG AACAGGGCCT CAGTCCTATG TAGAAGCCCT CCCTACTATT CAAGGCTACT
5281ATCGCCAGTA TCGTCAGGAG CCTGTCAGGT TTGGGAACAT CGGCTTCGGA ACCCCCTACT
5341ACTATGTGGG CTGGTACGAG TGTGGGGTCT CCATCCCTGG AAAGTGGTTG CCAACTTTCT
5401TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
5461CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
5521GTGGAAAGGA CGAGCCCCTG TTACTTCTAT GTGCCCTACG CGTTAAGTCg acaatcaacc
5581tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)