Construct: ORF TRCN0000479449
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002892.1_s317c1
- Derived from:
- ccsbBroadEn_12834
- DNA Barcode:
- GCCCCTGTTACTTCTATGTGCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FNDC1 (84624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479449
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84624 | FNDC1 | fibronectin type III domain... | NM_032532.3 | 93.3% | 93.2% | (many diffs) |
| 2 | human | 84624 | FNDC1 | fibronectin type III domain... | XM_011536190.2 | 92.1% | 92% | (many diffs) |
| 3 | human | 84624 | FNDC1 | fibronectin type III domain... | XM_011536191.2 | 91.1% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5388
- ORF length:
- 5319
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcctgaaa gtcacgtggg acccacccaa agatgctacc agtagacctg 121 tggagcatta caacattgcc tatgggaagt cactgaaaag tcttaaatac atcaaggtga 181 atgcggagac atactccttc cttattgagg atgtggagcc gggggtagtg tactttgtgc 241 tgcttactgc agaaaaccac agtggagtga gccgtcctgt ttacagagct gaaagcccac 301 ctggaggtga atggatcgag attgatggtt ttcccattaa gggtccagga ccatttaatg 361 aaaccgtcac agaaaaggaa gtgcccaaca agcccttgcg tgtgcgtgtc cggtcctcag 421 atgacaggct gtccgttgcg tggaaggcac cacgcctgtc tggagccaag agtccacgca 481 gatcacgggg ttttctcctg ggctacgggg agagtggccg gaagatgaat tatgttccac 541 tgacaagaga tgaacggaca cacgaaatta aaaagctagc ctcggaatcc gtgtatgtgg 601 tctccctgca gtccatgaac tctcagggcc ggagccaacc agtctacagg gctgccctaa 661 caaagcgaaa gatttcagaa gaggacgaat tggatgtacc tgacgacatc agcgtccggg 721 ttatgtcatc tcagtctgtg cttgtgtcct gggtggatcc tgttctggaa aaacagaaga 781 aagttgttgc atcaagacag tacaccgtgc gctatcgaga gaagggggaa ttggccaggt 841 gggattataa gcagatcgct aacaggcgtg tgctgattga gaacctgatt ccagacactg 901 tgtatgaatt tgcagtccgt atttcacagg gtgaaagaga tggcaaatgg agtacgtcag 961 tcttccaaag aacaccagaa tctgccccta ccacagctcc tgaaaacttg aacgtctggc 1021 cagtcaatgg caaacctaca gttgtcgctg catcttggga tgcgctacca gagactgagg 1081 ggaaagtgaa agccagtaag gcggatgttc agcagaacac ggaggacaat gggaaacccg 1141 aaaaacctga gccttcctca ccttctccca gagctccagc ttcctcccaa cacccctctg 1201 tgcctgcttc tccccaaggg agaaatgcca aggaccttct tcttgacttg aagaacaaaa 1261 tattggctaa tggtggggcg ccccgaaaac cccagcttcg cgccaagaag gcagaggagc 1321 tggatcttca gtcgacagaa atcactgggg aggaggagct gggttcccgg gaggactcgc 1381 ccatgtcacc ctcagacacc caagaccaga aacggaccct gaggccgcca agtagacacg 1441 gccactcggt ggttgctccc ggcaggactg cagtgagggc ccggatgcca gcgctgcccc 1501 gaagggaagg cgtagataag cctggctttt ccctggccac gcagccccgc ccaggggcgc 1561 ccccctcggc ttcggcctct cctgcccacc acgcgtccac ccagggcacc tctcatcgtc 1621 cttccctgcc tgccagcttg aatgacaacg acttggtgga ctcagacgaa gatgagcgcg 1681 ctgtgggctc cctccacccc aagggcgcct tcgcccagcc ccggccagcc ctgtccccca 1741 gccgccagtc cccgtccagc gttctccgcg acagaagctc tgtgcacccc ggcgcaaagc 1801 cagcctcgcc ggcccggagg accccccatt caggggccgc agaggaagat tccagtgcct 1861 cagccccacc ctcaagactt tctccacccc atgggggatc atctcggctg ctgcccaccc 1921 agccacacct gagctctcca ctttccaagg gcgggaagga tggtgaggac gccccagcca 1981 ccaactccaa tgcgccatca cggtccacca tgtcctcctc cgtctcttct catctctcgt 2041 ccaggacgca ggtctctgag ggagcggagg cttctgatgg tgaaagccac ggtgacggcg 2101 atagggaaga cggcggaagg caggcggagg ccacggccca gacgctgcgg gcccggcctg 2161 cctctggaca cttccatttg ctcagacaca aaccctttgc tgccaacggg aggtctccaa 2221 gcaggttcag cattgggcgg ggacctcggc tgcagccctc cagctcccca cagtcgactg 2281 tgccctcccg agcccacccc agggttccct ctcactctga ttcccaccct aagcttagct 2341 caggtatcca tggagacgag gaggatgaga agccgcttcc tgccaccgtt gtcaatgacc 2401 acgtgccttc ctcctccagg cagcccatct cccggggctg ggaggactta aggagaagcc 2461 cgcagagagg ggccagcctg catcggaagg aacccatccc agagaacccc aaatccacag 2521 gggcagatac acatcctcag ggcaagtact cctccctggc ctccaaggct caggatgttc 2581 aacagagcac agacgcggac acggagggtc attctcccaa agcacagcca gggtccacag 2641 accgccacgc gtcccctgct cgtcctcccg cagcacggtc acagcagcat cccagtgttc 2701 ccagaaggat gacacccggc cgggccccag aacagcagcc ccctcctccc gtcgccacgt 2761 cccagcacca cccgggaccc cagagcagag acgcgggtcg gtcaccttcc cagcccaggc 2821 tctcactgac ccaggccggg cggccccgcc ccacgtcgca gggccgctcc cactcctcct 2881 cggaccctta cacggcgagc tccagaggga tgctccccac ggccctccag aaccaggacg 2941 aggatgccca gggcagctac gacgacgaca gcacagaagt cgaggcccag gatgtgcggg 3001 cccccgcaca cgccgcgcgc gccaaggagg cagctgcgtc ccttcccaag caccagcagg 3061 tggagtctcc cacaggcgca ggggcaggtg gtgaccacag gtcccagcgc ggacatgcgg 3121 cctcccccgc caggcccagc cgacccggcg gcccccagtc ccgcgcccgg gtccccagca 3181 gggcagcgcc ggggaagtcg gagcctcctt ccaagcggcc cctgtcctcc aagtcccagc 3241 agtcggtctc agccgaggac gaggaggagg aggacgcggg gttttttaaa ggcgggaaag 3301 aagaccttct gtcttcctct gtgccaaagt ggccctcttc ctccactccc aggggcggca 3361 aagacgccga tgggagcctc gccaaggaag agagggagcc tgccatcgcg cttgcccctc 3421 gcggagggag cctggctcct gtgaagcgac ctctcccccc acctccaggc agctccccca 3481 gggcctccca cgtcccttcc cgaccgccgc ctcgcagcgc tgccaccgtg agccccgtcg 3541 cgggcaccca cccctggccg cggtacacca cgcgcgcccc gcctggccac ttctccacca 3601 ccccgatgct gtccttgcgc cagaggatga tgcatgccag attccgtaac cctctctccc 3661 gacagcctgc cagaccctct tacagacaag gttataatgg cagaccaaat gtagaaggga 3721 aagtccttcc tggtagtaat ggaaaaccga atggacagag aattatcaat ggccctcaag 3781 gaacaaagtg ggttgtggac cttgatcgtg ggttagtatt gaatgcagaa ggaaggtacc 3841 tccaagattc acatggaaat cctcttcgga ttaaactagg aggagatggt cgaaccattg 3901 tagatctgga agggaccccc gtggtgagtc ctgacggcct cccactcttt gggcaggggc 3961 gacatggcac acctctggcc aatgcccaag ataagccaat tttgagtctt ggaggaaagc 4021 cgctggtggg tttggaggtc atcaaaaaaa ccacccatcc ccctaccact accatgcagc 4081 ccaccactac tacgacgccc ctgcctacca ctacaacccc gaggcccacc actgccacca 4141 cccgccgcac gaccaccagg cgtccaacaa ccacagtccg aaccactacg cggacaacca 4201 ccaccaccac ccccaaaccc accactccca tccccacctg tccccctggg accttggaac 4261 ggcacgacga tgatggcaac ctgataatga gctccaatgg gatcccagag tgctacgctg 4321 aagaagatga gttctcaggc ttggagactg acactgcagt acctacggaa gaggcctacg 4381 ttatatatga tgaagattat gaatttgaga cgtcaaggcc accaaccacc actgagcctt 4441 cgaccactgc taccacaccg agggtgatcc cagaggaagg cgccatcagt tcctttcctg 4501 aagaagaatt tgatctggct ggaaggaaac gatttgttgc tccttacgtg acgtacctaa 4561 ataaagaccc atcagccccg tgctctctga ctgatgcact ggatcacttc caagtggaca 4621 gcctcgatga aatcatcccc aatgacctga agaagagtga cctgcctccc cagcatgctc 4681 cccgcaacat caccgtggtg gccgtggaag gttgccactc atttgtcatt gtggactggg 4741 acaaagctac cccaggagat gtggtcacag gttacttggt ttacagtgca tcctatgaag 4801 acttcatcag gaacaagtgg tccactcaag cttcatcagt aactcacttg cccattgaga 4861 acctaaagcc caacacgagg tattatttta aagtgcaagc acaaaatcct catggctacg 4921 gacctatcag cccttcggtc tcatttgtca ccgaatcaga taatcctctg cttgttgtga 4981 ggcccccagg cggtgagcct atctggatcc cattcgcttt caaacatgat cccagctaca 5041 cggactgcca tggacggcaa tatgtgaagc gcacgtggta tcgaaagttc gtgggagttg 5101 ttctttgtaa ttcactgagg tataaaatct acctcagtga caacctgaaa gatacaTTCT 5161 ACAGCATTGG AGACAGCTGG GGAAGAGGTG AAGACCATTG CCAATTTGTG GATTCACACC 5221 TTGATGGAAG AACAGGGCCT CAGTCCTATG TAGAAGCCCT CCCTACTATT CAAGGCTACT 5281 ATCGCCAGTA TCGTCAGGAG CCTGTCAGGT TTGGGAACAT CGGCTTCGGA ACCCCCTACT 5341 ACTATGTGGG CTGGTACGAG TGTGGGGTCT CCATCCCTGG AAAGTGGTTG CCAACTTTCT 5401 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 5461 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 5521 GTGGAAAGGA CGAGCCCCTG TTACTTCTAT GTGCCCTACG CGTTAAGTCg acaatcaacc 5581 tctggattac aaaatttgtg aaagatt