Transcript: Human XM_011536191.2

PREDICTED: Homo sapiens fibronectin type III domain containing 1 (FNDC1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FNDC1 (84624)
Length:
6192
CDS:
193..5526

Additional Resources:

NCBI RefSeq record:
XM_011536191.2
NBCI Gene record:
FNDC1 (84624)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011536191.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000436737 CCGAAGGGAAGGCGTAGATAA pLKO_005 1617 CDS 100% 13.200 18.480 N FNDC1 n/a
2 TRCN0000431864 GACTGCCATGGACGGCAATAT pLKO_005 5179 CDS 100% 13.200 18.480 N FNDC1 n/a
3 TRCN0000117930 CCTACTATTCAAGGCTACTAT pLKO.1 5398 CDS 100% 5.625 7.875 N FNDC1 n/a
4 TRCN0000117928 GCCCTAACAAAGCGAAAGATT pLKO.1 583 CDS 100% 5.625 3.938 N FNDC1 n/a
5 TRCN0000117929 CCTCCAAGATTCACATGGAAA pLKO.1 3957 CDS 100% 4.950 3.465 N FNDC1 n/a
6 TRCN0000117927 GCCTGGACAATGAACAGGATT pLKO.1 5704 3UTR 100% 4.950 3.465 N FNDC1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011536191.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12834 pDONR223 100% 91.1% 90.1% None (many diffs) n/a
2 ccsbBroad304_12834 pLX_304 0% 91.1% 90.1% V5 (many diffs) n/a
3 TRCN0000479449 GCCCCTGTTACTTCTATGTGCCCT pLX_317 4.5% 91.1% 90.1% V5 (many diffs) n/a
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