Construct: ORF TRCN0000479450
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002062.1_s317c1
- Derived from:
- ccsbBroadEn_12003
- DNA Barcode:
- CTGCAATGGTCACAGGGCTGTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMBIM4 (51643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479450
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51643 | TMBIM4 | transmembrane BAX inhibitor... | NM_001282609.1 | 28.3% | 22.2% | 97_134del;177_486del |
| 2 | human | 51643 | TMBIM4 | transmembrane BAX inhibitor... | NM_016056.3 | 19.3% | 15.1% | 97_134del;177_714del |
| 3 | human | 51643 | TMBIM4 | transmembrane BAX inhibitor... | NM_001282606.1 | 16.1% | 11.5% | 97_275del;318_855del |
| 4 | human | 51643 | TMBIM4 | transmembrane BAX inhibitor... | NM_001282610.1 | 12.2% | 4.1% | 0_1ins55;84_621del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 183
- ORF end:
- 321
- ORF length:
- 138
- Sequence:
-
1 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT 61 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT 121 TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG 181 GCATGGCTGA CCCCGACCCC CGGTACCCTC GCTCCTCGAT CGAGGACGAC TTCAACTATG 241 GCAGCAGCGT GGCCTCCGCC ACCGTGCACA TCCGAATGGG TTCTCTTAAC TACAGTGACT 301 TCAACAGTTT TTTTATACTT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 361 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 421 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTGC AATGGTCACA 481 GGGCTGTTTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt