Construct: ORF TRCN0000479461
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015595.1_s317c1
- Derived from:
- ccsbBroadEn_01501
- DNA Barcode:
- AAGCTGACAGAAATTTGGCCGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL14 (6358)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479461
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6358 | CCL14 | C-C motif chemokine ligand 14 | NM_032963.4 | 100% | 100% | |
| 2 | human | 6358 | CCL14 | C-C motif chemokine ligand 14 | NM_032962.4 | 85.3% | 84.4% | 80_127del |
| 3 | human | 348249 | CCL15-CCL14 | CCL15-CCL14 readthrough (NM... | NR_027922.3 | 19.7% | 1_989del;1269_1416del | |
| 4 | human | 348249 | CCL15-CCL14 | CCL15-CCL14 readthrough (NM... | NR_027921.3 | 19% | 1_989del;1069_1116del;1317_1464del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 369
- ORF length:
- 279
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAGATCTC CGTGGCTGCC ATTCCCTTCT 121 TCCTCCTCAT CACCATCGCC CTAGGGACCA AGACTGAATC CTCCTCACGG GGACCTTACC 181 ACCCCTCAGA GTGCTGCTTC ACCTACACTA CCTACAAGAT CCCGCGTCAG CGGATTATGG 241 ATTACTATGA GACCAACAGC CAGTGCTCCA AGCCCGGAAT TGTCTTCATC ACCAAAAGGG 301 GCCATTCCGT CTGTACCAAC CCCAGTGACA AGTGGGTCCA GGACTATATC AAGGACATGA 361 AGGAGAACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGCTG ACAGAAATTT GGCCGTTGAC 541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt