Construct: ORF TRCN0000479471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008158.1_s317c1
- Derived from:
- ccsbBroadEn_00258
- DNA Barcode:
- CCCGACCGCCGTAAACGATGTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD38 (952)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 952 | CD38 | CD38 molecule | NM_001775.4 | 100% | 100% | |
| 2 | human | 952 | CD38 | CD38 molecule | NR_132660.2 | 13.5% | 1_87del;450_451ins136;852_5484del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 966
- ORF length:
- 900
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caactgcgag ttcagcccgg tgtccgggga caaaccctgc tgccggctct 121 ctaggagagc ccaactctgt cttggcgtca gtatcctggt cctgatcctc gtcgtggtgc 181 tcgcggtggt cgtcccgagg tggcgccagc agtggagcgg tccgggcacc accaagcgct 241 ttcccgagac cgtcctggcg cgatgcgtca agtacactga aattcatcct gagatgagac 301 atgtagactg ccaaagtgta tgggatgctt tcaagggtgc atttatttca aaacatcctt 361 gcaacattac tgaagaagac tatcagccac taatgaagtt gggaactcag accgtacctt 421 gcaacaagat tcttctttgg agcagaataa aagatctggc ccatcagttc acacaggtcc 481 agcgggacat gttcaccctg gaggacacgc tgctaggcta ccttgctgat gacctcacat 541 ggtgtggtga attcaacact tccaaaataa actatcaatc ttgcccagac tGGAGAAAGG 601 ACTGCAGCAA CAACCCTGTT TCAGTATTCT GGAAAACGGT TTCCCGCAGG TTTGCAGAAG 661 CTGCCTGTGA TGTGGTCCAT GTGATGCTCA ATGGATCCCG CAGTAAAATC TTTGACAAAA 721 ACAGCACTTT TGGGAGTGTG GAAGTCCATA ATTTGCAACC AGAGAAGGTT CAGACACTAG 781 AGGCCTGGGT GATACATGGT GGAAGAGAAG ATTCCAGAGA CTTATGCCAG GATCCCACCA 841 TAAAAGAGCT GGAATCGATT ATAAGCAAAA GGAATATTCA ATTTTCCTGC AAGAATATCT 901 ACAGACCTGA CAAGTTTCTT CAGTGTGTGA AAAATCCTGA GGATTCATCT TGCACATCTG 961 AGATCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1021 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1081 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCGACCGC CGTAAACGAT GTCACACGCG 1141 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt