Construct: ORF TRCN0000479487
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013542.2_s317c1
- Derived from:
- ccsbBroadEn_07982
- DNA Barcode:
- CTAATTCACCGCTTTGAGGTGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APPL1 (26060)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479487
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26060 | APPL1 | adaptor protein, phosphotyr... | NM_012096.3 | 99.9% | 100% | 69A>G |
| 2 | human | 26060 | APPL1 | adaptor protein, phosphotyr... | XM_011533583.3 | 97.4% | 97.4% | 0_1ins52;2delT;18A>G |
| 3 | mouse | 72993 | Appl1 | adaptor protein, phosphotyr... | NM_145221.2 | 92.1% | 98% | (many diffs) |
| 4 | mouse | 72993 | Appl1 | adaptor protein, phosphotyr... | XM_006519608.1 | 89% | 94.6% | (many diffs) |
| 5 | mouse | 72993 | Appl1 | adaptor protein, phosphotyr... | XM_017316214.1 | 77.7% | 82.5% | (many diffs) |
| 6 | mouse | 72993 | Appl1 | adaptor protein, phosphotyr... | XM_006519609.3 | 69.3% | 73.2% | (many diffs) |
| 7 | mouse | 72993 | Appl1 | adaptor protein, phosphotyr... | XR_001781158.1 | 59.6% | (many diffs) | |
| 8 | mouse | 72993 | Appl1 | adaptor protein, phosphotyr... | XR_001781160.1 | 45.7% | (many diffs) | |
| 9 | mouse | 72993 | Appl1 | adaptor protein, phosphotyr... | XR_001781159.1 | 24.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2193
- ORF length:
- 2127
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggggatcgac aagctgccca tcgaggagac gctggaggac agcccgcaga 121 caaggtcttt actgggtgta tttgaagaag atgccacagc tatttccaac tatatgaacc 181 agttgtatca agctatgcat cggatttatg atgcacagaa tgaattaagt gcagcaacac 241 acctgacctc aaaactttta aaagaatatg aaaaacagcg ttttccattg ggaggtgatg 301 atgaagttat gagctctaca ttgcaacagt tttcaaaagt tatagatgag cttagctctt 361 gtcatgcagt gctttcaact caacttgctg atgccatgat gttccccatt acccagttta 421 aagaaagaga tctgaaagaa atactaacat taaaggaagt atttcagatt gcaagtaatg 481 atcatgatgc tgcgattaat agatatagcc gtttatcaaa aaaaagagaa aatgacaagg 541 tgaagtatga agtaacagaa gatgtgtaca catccagaaa gaaacaacac cagaccatga 601 tgcattattt ttgtgcatta aatactcttc agtacaagaa gaaaatagca ttgttagaac 661 ctctacttgg gtacatgcaa gctcagataa gtttctttaa gatgggttct gaaaatctta 721 atgaacaact ggaagaattt ttagctaata ttggaacaag cgttcagaat gttcgcaggg 781 aaatggacag tgatatagag accatgcaac agacaataga ggatttggaa gtagccagtg 841 atcccttata tgtgcctgac ccagacccca ccaaatttcc tgttaatcga aatttaaccc 901 gaaaggctgg ataccttaat gctaggaata aaacaggctt ggtgtcatct acctgggaca 961 gacagtttta cttcacgcag ggtggaaatt taatgagtca ggcccgtggg gatgtagcag 1021 gaggcctggc catggacata gacaactgtt cagtgatggc tgtggactgt gaagacagac 1081 gatattgttt tcagatcacc tctttcgatg gaaaaaaatc ttcaattttg caagcagaga 1141 gtaaaaaaga tcatgaagag tggatctgta caataaacaa catatctaaa caaatatact 1201 taagtgaaaa tccagaggaa actgctgcac gagtaaatca atcagctctg gaagctgtca 1261 ctccttcccc atctttccag cagaggcacg agagcctgcg gccagcagca ggacaatctc 1321 ggccaccgac agctcgaacc agcagttcag gatccttagg atctgagtct acaaatttgg 1381 ctgccctctc tctagattct cttgttgccc cagacacccc aatacagttt gacataattt 1441 ctcctgtgtg tgaagatcag cctggccagg caaaagcctt tggccaggga ggcaggcgta 1501 caaatccatt tggagaatct ggaggaagta caaaatctga aactgaagat tctattcttc 1561 atcagttatt tattgtccga ttccttggtt caatggaggt gaaatcagat gaccatccag 1621 atgttgttta tgaaactatg cgccaaatct tagctgcccg ggccatccat aacatctttc 1681 gtatgacaga atcgcattta ttagtcactt gtgactgttt aaagttaatt gatccacaga 1741 cacaagttac aaggctcacg tttccattac cttgtgtagt tttgtatgct acacaccagg 1801 aaaataagcg cctttttGGA TTTGTTCTTC GGACATCAAG CGGGAGAAGT GAAAGTAATC 1861 TGTCATCAGT CTGCTATATA TTTGAGTCAA ACAATGAGGG GGAAAAGATA TGTGATTCTG 1921 TTGGACTGGC AAAACAGATA GCTTTGCATG CTGAACTGGA TCGTAGGGCA TCAGAAAAAC 1981 AAAAAGAAAT AGAGAGAGTA AAAGAGAAGC AACAGAAAGA ACTCAATAAA CAAAAACAGA 2041 TTGAAAAGGA CTTGGAAGAA CAAAGTCGGT TGATAGCTGC TTCCAGTAGA CCAAACCAAG 2101 CCAGTAGTGA GGGGCAGTTT GTTGTCCTTA GCAGTAGCCA GTCAGAAGAG AGTGATTTGG 2161 GAGAAGGAGG AAAGAAGAGA GAATCAGAAG CATACCCAAC TTTCTTGTAC AAAGTGGTTG 2221 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2281 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 2341 AATTCACCGC TTTGAGGTGA CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2401 ttgtgaaaga tt