Construct: ORF TRCN0000479490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003341.1_s317c1
- Derived from:
- ccsbBroadEn_13815
- DNA Barcode:
- AACCCCACGGACCGCCCTGATTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARR3 (407)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479490
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 407 | ARR3 | arrestin 3 | NM_004312.3 | 92.4% | 92% | 132A>T;1076_1081delGCTCTG;1084_1164del |
| 2 | human | 407 | ARR3 | arrestin 3 | XM_017029518.1 | 88.9% | 88.5% | (many diffs) |
| 3 | mouse | 170735 | Arr3 | arrestin 3, retinal | NM_133205.3 | 80.5% | 79.5% | (many diffs) |
| 4 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_011247529.1 | 77.5% | 76.5% | (many diffs) |
| 5 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_017318401.1 | 72.8% | 71.2% | (many diffs) |
| 6 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_017318400.1 | 68% | 66.1% | (many diffs) |
| 7 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527870.2 | 67.9% | 65.7% | (many diffs) |
| 8 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_017318399.1 | 66.9% | 65.6% | (many diffs) |
| 9 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527867.2 | 66.8% | 65.4% | (many diffs) |
| 10 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527866.2 | 66.8% | 65.3% | (many diffs) |
| 11 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527865.2 | 66.1% | 64.7% | (many diffs) |
| 12 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527871.2 | 65.4% | 64.1% | (many diffs) |
| 13 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527869.2 | 64.7% | 63.4% | (many diffs) |
| 14 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_017318398.1 | 62.8% | 61.2% | (many diffs) |
| 15 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527862.2 | 62.7% | 60.9% | (many diffs) |
| 16 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527863.2 | 61.4% | 59.7% | (many diffs) |
| 17 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527872.2 | 59.6% | 58.2% | (many diffs) |
| 18 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527864.2 | 57.8% | 56% | (many diffs) |
| 19 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527868.2 | 56.5% | 54.8% | (many diffs) |
| 20 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527875.3 | 47.1% | 45.5% | (many diffs) |
| 21 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_017318402.1 | 47.1% | 45.5% | (many diffs) |
| 22 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_017318403.1 | 47.1% | 45.5% | (many diffs) |
| 23 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_017318404.1 | 47.1% | 45.5% | (many diffs) |
| 24 | mouse | 170735 | Arr3 | arrestin 3, retinal | XM_006527873.2 | 41.1% | 39.1% | (many diffs) |
| 25 | mouse | 170735 | Arr3 | arrestin 3, retinal | XR_386916.2 | 40.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caaggtgttt aagaagacca gctccaatgg gaagctctcc atctacctgg 121 ggaaacggga cttcgtggac catgtggaca cggtggaacc cattgacggt gttgtcctgg 181 ttgatcctga gtactttaaa tgtcgaaagt tgtttgtcat gttgacatgt gcctttcgct 241 atggccgtga tgacttggaa gtgattggtc tgacgttccg aaaagatctg tatgtgcaga 301 ccctgcaagt ggtcccagct gaatccagca gccctcaggg gcccctcaca gtcctacagg 361 agcgactact gcacaagcta ggggacaatg cctacccctt taccctgcag atggtgacca 421 acctgccctg ttctgtgaca ctgcagccag gtcctgaaga tgcaggaaag ccctgtggga 481 ttgactttga agtgaagagt ttctgtgctg aaaacccaga ggagacagtc tccaagagag 541 actatgtgcg gctggttgtc cggaaagtac aatttgcacc accggaggca ggccctggcc 601 cctcagccca gaccatccgc cgcttccttc tgtcagctca gcccctacaa ctccaggcct 661 ggatggacag ggaggttcac taccacggag aacccatctc tgtcaatgtt tctatcaaca 721 actgcaccaa caaggtcatc aaaaaaatca agatttCAGT TGACCAGATC ACAGATGTTG 781 TCCTGTATTC ACTAGACAAG TACACCAAGA CTGTGTTCAT TCAGGAATTC ACGGAGACTG 841 TAGCTGCTAA TTCCAGCTTC TCCCAGAGCT TTGCAGTAAC CCCAATCCTG GCTGCCAGCT 901 GCCAGAAACG GGGCCTGGCA CTGGATGGCA AACTTAAGCA TGAAGATACC AACCTGGCCT 961 CTAGCACAAT TATTAGACCG GGAATGGACA AAGAGCTGCT GGGGATCCTG GTGTCCTACA 1021 AAGTCAGAGT CAACCTGATG GTGTCCTGTG GTGGCATCCT AGGAGACCTG ACAGCCAGCG 1081 ATGTTGGTGT GGAGCTACCC TTGGTCCTGA TCCATCCGAA GCCATCTCAT GAGGCCGCTA 1141 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CCCCACGGAC CGCCCTGATT CGACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt