Construct: ORF TRCN0000479492
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001493.2_s317c1
- Derived from:
- ccsbBroadEn_11725
- DNA Barcode:
- AGACACCAACCGTTCTGATGTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- U2SURP (23350)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479492
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23350 | U2SURP | U2 snRNP associated SURP do... | NM_001320220.2 | 99.9% | 100% | 78A>T |
| 2 | human | 23350 | U2SURP | U2 snRNP associated SURP do... | XM_017006038.1 | 99.9% | 100% | 78A>T |
| 3 | human | 23350 | U2SURP | U2 snRNP associated SURP do... | XM_017006039.1 | 99.9% | 100% | 78A>T |
| 4 | human | 23350 | U2SURP | U2 snRNP associated SURP do... | NM_001320222.2 | 96% | 96.1% | 0_1ins72;6A>T |
| 5 | human | 23350 | U2SURP | U2 snRNP associated SURP do... | XM_024453425.1 | 79% | 77.4% | 1_448del;493_536del;570A>T |
| 6 | human | 23350 | U2SURP | U2 snRNP associated SURP do... | NM_001320219.2 | 60.2% | 59% | 1_1180del;1225_1268del;1302A>T |
| 7 | human | 23350 | U2SURP | U2 snRNP associated SURP do... | NM_001080415.2 | 60.2% | 58.9% | 1_1183del;1228_1271del;1305A>T |
| 8 | human | 23350 | U2SURP | U2 snRNP associated SURP do... | XM_005247248.5 | 54.4% | 53.2% | (many diffs) |
| 9 | mouse | 67958 | U2surp | U2 snRNP-associated SURP do... | XM_017313543.1 | 91.5% | 98.7% | (many diffs) |
| 10 | mouse | 67958 | U2surp | U2 snRNP-associated SURP do... | XM_017313544.1 | 91.5% | 98.7% | (many diffs) |
| 11 | mouse | 67958 | U2surp | U2 snRNP-associated SURP do... | XM_017313545.1 | 91.5% | 98.7% | (many diffs) |
| 12 | mouse | 67958 | U2surp | U2 snRNP-associated SURP do... | XM_017313542.1 | 57.6% | 61.2% | (many diffs) |
| 13 | mouse | 67958 | U2surp | U2 snRNP-associated SURP do... | NM_026476.2 | 57.5% | 61.2% | (many diffs) |
| 14 | mouse | 67958 | U2surp | U2 snRNP-associated SURP do... | XM_006511384.3 | 55.1% | 58.6% | (many diffs) |
| 15 | mouse | 67958 | U2surp | U2 snRNP-associated SURP do... | NM_001114977.1 | 55.1% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1929
- ORF length:
- 1860
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctcctatgt taccgccacc taaaaacaaa gaggattttg agaaggaatt 121 tgctcgccct gatacatcga atgattgagt ttgttgtacg tgaagggcca atgtttgaag 181 ctatgattat gaacagagaa atcaacaatc ctatgttcag gttcttattt gaaaaccaga 241 caccagccca tgtttactat aggtggaagc tttattctat tctgcaggga gattctccaa 301 ctaaatggcg gacggaagat tttcgtatgt tcaaaaatgg atctttttgg aggccaccac 361 cattaaatcc gtacttgcat ggaatgtcag aagagcaaga aacagaagct tttgtagagg 421 aacctagtaa aaagggagca cttaaggaag aacagaggga taaattggaa gaaatcttgc 481 ggggattaac tccaaggaaa aatgatattg gagatgcaat ggttttctgt cttaataatg 541 ctgaagctgc tgaagaaata gtggattgca ttactgagtc gttgtccatc ttaaagacac 601 cccttcctaa aaagattgcc agattatatt tggtttctga tgttttgtac aactcttcag 661 ccaaagttgc taatgcttca tattatagaa aattttttga aacaaagtta tgtcagatat 721 tttcagacct caatgccacc tatcgtacaa ttcaaggcca tttacaatct gaaaacttta 781 agcaacgggt aatgacttgc ttcagagcat gggaagattg ggcaatttat ccagaaccat 841 ttttgatcaa actacaaaat attttcttag gacttgtaaa tattattgaa gaaaaggaaa 901 cagaggatgt tccagatgac cttgatggtg cccccatcga ggaagagctt gatggtgcac 961 ctctggaaga tgtagatgga attcctattg atgctactcc catcgatgat cttgatggag 1021 tccctataaa aagtcttgat gatgatcttg atggagtgcc tttggatgca actgaagact 1081 caaaaaagaa tgagcctata tttaaagttg ccccatcaaa atgggaagct gtggatgaat 1141 ctgaattgga agcacaggct gttacaactt ctaaatggga attatttgac cagcatgaag 1201 aatcagaaga agaagaaaat caaaatcaag aagaagaaag tgaagatgaa gaagatactc 1261 aaagttccaa atctgaagaa catcatttgt actctaatcc aatcaaagaa gaaatgactg 1321 agtctaagtt ctctaagtac tctgaaatga gtgaggaaaa acgagccaaa cttcgtgaaa 1381 ttgagctcaa agttatgaag tttcaggatg aattggaatc tgggaaaaga cctaaaaaac 1441 caggccagag ttttcaggag caagtagaac actacagaga taaacttctt caacgagaga 1501 aagagaaaga gttagaaaga gaacgagaaa gagacaagaa agataaagaa aaattggaat 1561 ctcgctccaa agacaagaag gaaaaagatg agtgtactcc gacaaggaag gaaaggaaga 1621 ggcgacacag tacaTCCCCC AGCCCATCTC GCAGTAGCAG TGGTAGACGA GTGAAATCCC 1681 CATCACCAAA ATCGGAGCGA TCAGAGCGTT CAGAAAGATC TCATAAAGAG AGCTCACGGT 1741 CCAGGTCATC TCACAAAGAT TCTCCTAGAG ATGTTAGCAA AAAAGCCAAA AGATCACCAT 1801 CTGGTTCAAG GACACCTAAA AGGTCTAGGC GATCACGGTC TAGATCTCCT AAAAAATCAG 1861 GAAAGAAGTC CAGATCCCAG TCCAGATCTC CACACAGGTC TCATAAAAAG TCAAAGAAAA 1921 ACAAACACTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1981 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2041 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGACAC CAACCGTTCT GATGTGGTAC 2101 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt