Construct: ORF TRCN0000479512
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010078.1_s317c1
- Derived from:
- ccsbBroadEn_09442
- DNA Barcode:
- TATATGTGCACAGTCTACTCGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CATSPER2 (117155)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479512
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 117155 | CATSPER2 | cation channel sperm associ... | NR_110319.1 | 38.2% | (many diffs) | |
| 2 | human | 117155 | CATSPER2 | cation channel sperm associ... | NM_001282309.3 | 36.9% | 36% | (many diffs) |
| 3 | human | 117155 | CATSPER2 | cation channel sperm associ... | NM_172095.4 | 36.8% | 35.9% | (many diffs) |
| 4 | human | 117155 | CATSPER2 | cation channel sperm associ... | NM_001282310.2 | 36.5% | 35.6% | (many diffs) |
| 5 | human | 440278 | CATSPER2P1 | cation channel sperm associ... | NR_002318.2 | 30% | (many diffs) | |
| 6 | human | 110006325 | PPIP5K1P1-CATSPER2 | PPIP5K1P1-CATSPER2 readthrough | NR_146339.1 | 11% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcttaccaa caagaagagc agatgcagct tccccgagct gatgccattc 121 gttcacgtct catcgatact ttctctctca ttgagcattt gcaaggcttg agccaagctg 181 tgccgcggca cactatcagg aagttacttg atccttcccg ccagaagaaa cttgtattgg 241 gagatcaaca ccagctagtg cgtttctcta taaagccTCA GCGTATAGAA CAGATTTCAC 301 ATGCCCAGAG GCTGTTGAGC AGGCTTCATG TGCGCTGCAG TCAGAGGCCA CCTCTTTCTT 361 TGTGGGCCGG ATGGGTCCTT GAGTGTCCTC TCTTCAAAAA CTTCATCATC TTCCTGGTCT 421 TTTTGAATAC GATCATATTG ATGGTTGAAA TAGAATTGCT GGAATCCACA AATACCAAAC 481 TATGGCCATT GAAGCTGACC TTGGAGGTGG CAGCTTGGTT TATCTTGCTT ATTTTCATCC 541 TGGAGATCCT TCTTAAGTGG CTATCCAACT TTTCTGTTTT CTGGAAGAGT GCCTGGAATG 601 TCTTTGACTT TGTTGTTACC ATGTTGGTAA GGATAGAGAT CCTGAGGGTT CGTTTAGTGG 661 GATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGATA TATGTGCACA GTCTACTCGT GTACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt