Construct: ORF TRCN0000479514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006865.1_s317c1
- Derived from:
- ccsbBroadEn_08157
- DNA Barcode:
- ACGTGCCTTATCCCCTGGGAAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOP53 (29997)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29997 | NOP53 | NOP53 ribosome biogenesis f... | NM_015710.5 | 99.7% | 99.7% | 705A>G;1166A>G;1221G>A |
| 2 | human | 440311 | LOC440311 | NOP53 ribosome biogenesis f... | NR_077061.1 | 79.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcaggaggc agtggcgttg gtgggaagcg cagctcgaaa agcgatgccg 121 attctggttt cctggggctg cggcccactt cggtggaccc agcgctgagg cggcggcggc 181 gaggcccaag aaataagaag cggggctggc ggcggcttgc tcaggagccg ctggggctgg 241 aggttgacca gttcctggaa gacgtgcggc tacaggagcg cacgagcggt ggcttgttgt 301 cagaggcccc aaatgaaaaa ctcttcttcg tggacactgg ctccaaggaa aaagggctga 361 caaagaagag aaccaaagtc cagaagaagt cactgcttct caagaaaccc cttcgggttg 421 acctcatcct cgagaacaca tccaaagtcc ctgcccccaa agacgtcctc gcccaccagg 481 tccccaacgc caagaagctc aggcggaagg agcagctatg ggagaagctg gccaagcagg 541 gcgagctgcc ccgggaggtg cgcagggccc aggcccggct cctcaaccct tctgcaacaa 601 gggccaagcc cgggccccag gacaccgtag agcggccctt ctacgacctc tgggcctcag 661 acaaccccct ggacaggccg ttggttggcc aggatgagtt tttcctggag cagaccaaga 721 agaaaggagt gaagcggcca gcacgcctgc acaccaagcc gtcccaggcg cccgccgtgg 781 aggtggcgcc tgccggagct tcctacaatc catcctttga agaccaccag accctgctct 841 cagcggccca cgaggtggag ttgcagcggc agaaggaggc ggagaagctg gagcggcagc 901 tggccctgcc cgccacggag caggccgcca cccaggagtc cacattccag gagctgtgcg 961 aggggctgct ggaggagtcg gatggtgagg gggagccagg ccagggcgag gggccggagg 1021 ctggggatgc cgaggtctgt cccacgcccg cccgcctggc caccacagag aagaagacgg 1081 agcagcagcg gcggcgggag aaggctgtgc acaggctgcg ggtacagcag gccgcgttgc 1141 gggccgcccg gcTCCGGCAC CAGGAGCTGT TCCGGCTGCG CGGGATCAAG GCCCAGGTGG 1201 CCCTGAGGCT GGCGGAGCTG GCGCGGCGGC GGAGGCGGCG GCAGGCGCGG CGGGAGGCTG 1261 AGGCTGACAA GCCCCGAAGG CTGGGACGGC TCAAGTACCA GGCACCTGAC ATCGACGTGC 1321 AGCTGAGCTC GGAGCTGACA GACTCGCTCA GGACCCTGAA GCCCGAGGGC AACATCCTTC 1381 GAGACCGGTT CAAGAGCTTC CAGAGGAGGA ATATGATCGA GCCTCGAGAG AGAGCCAAGT 1441 TCAAACGCAA GTACAAGGTG AAGCTGGTGG AGAAGCGGGC GTTCCGTGAG ATCCAGTTGT 1501 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGAACGTG CCTTATCCCC TGGGAAAAGA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt