Construct: ORF TRCN0000479523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014470.1_s317c1
- Derived from:
- ccsbBroadEn_08608
- DNA Barcode:
- ATTCAACGTGTGCAAATACCGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UNC45A (55898)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479523
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55898 | UNC45A | unc-45 myosin chaperone A | NM_001323619.1 | 99.9% | 100% | 612G>C |
| 2 | human | 55898 | UNC45A | unc-45 myosin chaperone A | NM_018671.5 | 99.9% | 100% | 612G>C |
| 3 | human | 55898 | UNC45A | unc-45 myosin chaperone A | NM_001039675.1 | 98.3% | 98.1% | 6_7ins45;567G>C |
| 4 | human | 55898 | UNC45A | unc-45 myosin chaperone A | NM_001323621.1 | 98.3% | 98.1% | 6_7ins45;567G>C |
| 5 | human | 55898 | UNC45A | unc-45 myosin chaperone A | NM_001323620.1 | 84.6% | 84.6% | 0_1ins435;177G>C |
| 6 | human | 55898 | UNC45A | unc-45 myosin chaperone A | XM_005254963.4 | 84.6% | 84.6% | 0_1ins435;177G>C |
| 7 | human | 55898 | UNC45A | unc-45 myosin chaperone A | XM_024449983.1 | 84.6% | 84.6% | 0_1ins435;177G>C |
| 8 | human | 55898 | UNC45A | unc-45 myosin chaperone A | XM_024449984.1 | 84.6% | 84.6% | 0_1ins435;177G>C |
| 9 | human | 55898 | UNC45A | unc-45 myosin chaperone A | XM_024449985.1 | 84.6% | 84.6% | 0_1ins435;177G>C |
| 10 | human | 55898 | UNC45A | unc-45 myosin chaperone A | XM_011521780.3 | 53.6% | 53.6% | 0_1ins1314 |
| 11 | human | 55898 | UNC45A | unc-45 myosin chaperone A | XM_017022407.2 | 53.6% | 53.6% | 0_1ins1314 |
| 12 | human | 55898 | UNC45A | unc-45 myosin chaperone A | XM_024449986.1 | 53.6% | 53.6% | 0_1ins1314 |
| 13 | mouse | 101869 | Unc45a | unc-45 myosin chaperone A | NM_133952.2 | 88.8% | 94% | (many diffs) |
| 14 | mouse | 101869 | Unc45a | unc-45 myosin chaperone A | XM_011250774.1 | 88.8% | 94% | (many diffs) |
| 15 | mouse | 101869 | Unc45a | unc-45 myosin chaperone A | XM_011250775.1 | 87.5% | 92.5% | (many diffs) |
| 16 | mouse | 101869 | Unc45a | unc-45 myosin chaperone A | XM_006540542.2 | 75% | 80% | (many diffs) |
| 17 | mouse | 101869 | Unc45a | unc-45 myosin chaperone A | XM_017321917.1 | 75% | 80% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2898
- ORF length:
- 2832
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgtgagtggt ccagggaccc ccgagccccg gccggccacc cccggggcca 121 gctcagtgga gcagctgcgg aaggagggca atgagctgtt caaatgtgga gactacgggg 181 gcgccctggc ggcctacact caggccctgg gtctggacgc gacgccccag gaccaggccg 241 ttctgcaccg gaaccgggcc gcctgccacc tcaagctgga agattacgac aaagcagaaa 301 cagaggcatc caaagccatt gaaaaggatg gtggggatgt caaagcactc taccggcgga 361 gccaagccct agagaagctg ggccgcctgg accaggctgt ccttgacctg cagagatgtg 421 tgagcttgga gcccaagaac aaagttttcc aggaggcctt gcggaacatc gggggccaga 481 ttcaggagaa ggtgcgatac atgtcctcga cggatgccaa agtggaacag atgtttcaga 541 tactgttgga cccagaagag aagggcactg agaaaaagca aaaggcttct cagaacctgg 601 tggtgctggc cagggaggat gctggagcgg agaagatctt ccggagtaat ggggttcagc 661 tcttgcaacg tttactcgac atgggagaga ctgacctcat gctggcggct ctgcgtacgc 721 tggttggcat ttgctctgag catcagtcac ggacagtggc aaccctgagc atactgggaa 781 ctcggcgagt agtctccatc ctgggcgtgg aaagccaggc tgtgtccctg gctgcctgcc 841 acctgctgca ggttatgttt gatgccctca aggaaggtgt caaaaaaggc ttccgaggca 901 aagaaggtgc catcattgtg gatcctgccc gggagctgaa ggtcctcatc agtaacctct 961 tagatctgct gacagaggtg ggggtctctg gccaaggccg agacaatgcc ctgaccctcc 1021 tgattaaagc ggtgccccgg aagtctctca aggaccccaa caacagcctc accctctggg 1081 tcatcgacca aggtctgaaa aagattttgg aagtgggggg ctctctacag gaccctcctg 1141 gggagctcgc agtgaccgca aacagccgca tgagcgcctc tattctcctc agcaagctct 1201 ttgatgacct caagtgtgat gcggagaggg agaatttcca cagactttgt gaaaactaca 1261 tcaagagctg gtttgagggc caagggctgg ccgggaagct acgggccatc cagacggtgt 1321 cctgcctcct gcagggccca tgtgacgctg gcaaccgggc cttggagctg agcggtgtca 1381 tggagagtgt gattgctctg tgtgcctctg agcaggagga ggagcagctg gtggccgtgg 1441 aggctctgat ccatgcagcc ggcaaggcta agcgggcctc attcatcact gccaatggtg 1501 tctcgctgct gaaggaccta tataagtgca gcgagaagga cagcatccgc atccgggcgc 1561 tagtgggact ctgtaagctc ggttcggctg gagggactga cttcagcatg aagcagtttg 1621 ctgaaggctc cactctcaaa ctggctaagc agtgtcgaaa gtggctgtgc aatgaccaga 1681 tcgacgcagg cactcggcgc tgggcagtgg agggcctggc ttacctgacc tttgatgccg 1741 acgtgaagga agagtttgtg gaggatgcgg ctgctctgaa agctctgttc cagctcagca 1801 ggttggagga gaggtcagtg ctctttgcgg tggcctcagc gctggtgaac tgcaccaaca 1861 gctatgacta cgaggagccc gaccccaaga tggtggagct ggccaagtat gccaagcagc 1921 atgtgcccga gcagcacccc aaggacaagc caagcttcgt gcgggctcgg gtgaagaagc 1981 tgctggcagc gggtgtggtg tcggccatgg tgtgcatggt gaagacggag agccctgtgc 2041 tgaccagttc ctgcagagag ctgctctcca gggtcttctt ggctttagtg gaagaggtag 2101 aggaccgagg cactgtggtt gcccagggag gcggcagggc gctgatcccg ctggccctgg 2161 aaggcacgga cgtggggcag acaaaggcag cccaggccct tgccaagctc accatcacct 2221 ccaacccgga gatgaccttc cctggcgagc ggatctatga ggtggtccgg cccctcgtct 2281 ccctgttgca cctcaactgc tcaggcctgc agaacttcga ggcgctcatg gccctaacaa 2341 acctggctgg gatcagcgag aggctccggc agaagatcct gaaggagaag gctgtgccca 2401 tgatagaagg ctacatgttt gaggagcatg agatgatccg ccgggcagcc acggagtgca 2461 tgtgtaactt ggccatgagc aaggaggtgc aggacctctt cgaagccCAG GGCAATGACC 2521 GACTGAAGCT GCTGGTGCTG TACAGTGGAG AGGATGATGA GCTGCTACAG CGGGCAGCTG 2581 CCGGGGGCTT GGCCATGCTT ACCTCCATGC GGCCCACGCT CTGCAGCCGC ATTCCCCAAG 2641 TGACCACACA CTGGCTGGAG ATCCTGCAGG CCCTGCTTCT GAGCTCCAAC CAGGAGCTGC 2701 AGCACCGGGG TGCTGTGGTG GTGCTGAACA TGGTGGAGGC CTCGAGGGAG ATTGCCAGCA 2761 CCCTGATGGA GAGTGAGATG ATGGAGATCT TGTCAGTGCT AGCTAAGGGT GACCACAGCC 2821 CTGTCACAAG GGCTGCTGCA GCCTGCCTGG ACAAAGCAGT GGAATATGGG CTTATCCAAC 2881 CCAACCAAGA TGGAGAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2941 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3001 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATTCAAC GTGTGCAAAT 3061 ACCGGCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt