Construct: ORF TRCN0000479526
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001946.1_s317c1
- Derived from:
- ccsbBroadEn_13204
- DNA Barcode:
- AACCACAACCCTTTTGAAAAGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM37 (140738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479526
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140738 | TMEM37 | transmembrane protein 37 | XM_005263598.4 | 100% | 100% | |
| 2 | human | 140738 | TMEM37 | transmembrane protein 37 | XM_006712300.3 | 100% | 100% | |
| 3 | human | 140738 | TMEM37 | transmembrane protein 37 | NM_183240.3 | 54.2% | 54.2% | 1_261del |
| 4 | human | 140738 | TMEM37 | transmembrane protein 37 | XM_011510659.2 | 50.9% | 50.9% | 1_297del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 399
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGCCTGGT ACGCAGCGTG GGCGCCTTGG 121 CCGTGGTGGC CGCCATTTTT GGCCTGGAGT TCCTCATGGT GTCCCAGTTG TGCGAGGACA 181 AACACTCACA GTGCAAGTGG GTCATGGGTT CCATCCTCCT CCTGGTGTCT TTCGTCCTCT 241 CCTCCGGCGG GCTCCTGGGT TTTGTGATCC TCCTCAGGAA CCAAGTCACA CTCATCGGCT 301 TCACCCTAAT GTTTTGGTGC GAATTCACTG CCTCCTTCCT CCTCTTCCTG AACGCCATCA 361 GCGGCCTTCA CATCAACAGC ATCACCCATC CCTGGGAATG CCCAACTTTC TTGTACAAAG 421 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 481 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 541 ACGAAACCAC AACCCTTTTG AAAAGCCTAC GCGTTAAGTC gacaatcaac ctctggatta 601 caaaatttgt gaaagatt