Construct: ORF TRCN0000479547
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014809.1_s317c1
- Derived from:
- ccsbBroadEn_03015
- DNA Barcode:
- CCCAATACTACGTTTTATTGCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSDC2 (27254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479547
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27254 | CSDC2 | cold shock domain containin... | NM_014460.4 | 100% | 100% | |
| 2 | mouse | 105859 | Csdc2 | cold shock domain containin... | NM_145473.3 | 88.3% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ttcagagtcg acgtcacccc cagttgtgcc cccgctccac tcccccaagt 121 ccccagtctg gcccaccttc cccttccaca gggagggcag cagggtctgg gagcggggtg 181 gtgtcccacc tcgggaccta cccagccctc tgcccaccaa gcggaccagg acctattcag 241 cgacagcccg ggccTCAGCT GGCCCCGTGT TCAAGGGCGT CTGTAAGCAG TTCTCACGCT 301 CACAGGGCCA TGGCTTCATC ACCCCCGAGA ACGGGTCCGA GGACATCTTC GTACATGTGT 361 CTGACATCGA GGGGGAGTAC GTGCCAGTGG AGGGCGACGA GGTGACCTAC AAGATGTGCC 421 CTATCCCTCC CAAGAACCAG AAGTTCCAGG CCGTGGAGGT GGTGCTCACT CAGCTGGCCC 481 CCCACACTCC CCACGAGACG TGGTCTGGCC AGGTCGTGGG CTCCTACCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA CCCAATACTA CGTTTTATTG CGATACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt