Construct: ORF TRCN0000479559
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006391.1_s317c1
- Derived from:
- ccsbBroadEn_03751
- DNA Barcode:
- CAGCCTTTGGTGACTGTGTGCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA4L2 (56901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479559
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56901 | NDUFA4L2 | NDUFA4 mitochondrial comple... | NM_020142.3 | 100% | 100% | |
| 2 | mouse | 407790 | Ndufa4l2 | NADH dehydrogenase (ubiquin... | NM_001098789.1 | 88.8% | 94.2% | (many diffs) |
| 3 | mouse | 407790 | Ndufa4l2 | NADH dehydrogenase (ubiquin... | XM_006513834.3 | 75.3% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 351
- ORF length:
- 261
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCAGGAGC CAGTCTTGGG GCCCGCTTCT 121 ACCGGCAGAT CAAAAGACAT CCGGGGATCA TCCCGATGAT CGGCTTAATC TGCCTGGGCA 181 TGGGCAGCGC TGCGCTTTAC TTGCTGCGAC TCGCCCTTCG CAGCCCCGAC GTCTGCTGGG 241 ACAGAAAGAA CAACCCGGAG CCCTGGAACC GCCTGAGCCC CAATGACCAA TACAAGTTCC 301 TTGCAGTTTC CACTGACTAT AAGAAGCTGA AGAAGGACCG GCCAGACTTC TACCCAACTT 361 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 421 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 481 CTTGTGGAAA GGACGACAGC CTTTGGTGAC TGTGTGCTCA ACGCGTTAAG TCgacaatca 541 acctctggat tacaaaattt gtgaaagatt