Construct: ORF TRCN0000479597
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018945.2_s317c1
- Derived from:
- ccsbBroadEn_14800
- DNA Barcode:
- ACAATAAGTATTACTCAAATACAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAPK3 (5595)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479597
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5595 | MAPK3 | mitogen-activated protein k... | NM_002746.3 | 99.6% | 35.3% | (many diffs) |
| 2 | human | 5595 | MAPK3 | mitogen-activated protein k... | NM_001040056.3 | 92.5% | 37.5% | (many diffs) |
| 3 | human | 5595 | MAPK3 | mitogen-activated protein k... | NM_001109891.1 | 88% | 39.9% | (many diffs) |
| 4 | human | 5595 | MAPK3 | mitogen-activated protein k... | XR_243293.1 | 58.8% | (many diffs) | |
| 5 | mouse | 26417 | Mapk3 | mitogen-activated protein k... | NM_011952.2 | 89.6% | 33.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 489
- ORF length:
- 420
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcggcg gcggctcagg ggggcggggg cggggagccc cgtagaaccg 121 agggggtcgg cccgggggtc ccgggggagg tggagatggt gaaggggcag ccgttcgacg 181 tgggcccgcg ctacacgcag ttgcagtaca tcggcgaggg cgcgtacggc atggtcagct 241 cggcctatga ccacgtgcgc aagactcgcg tggccatcaa gaagatcagc cccttcgaac 301 atcagaccta ctgccagcgc acgctccggg agatccagat cctgctgcgc ttccgccatg 361 agaatgtcat cggcatccga gacattctgc gggcgtccac cctggaagcc atgagagatg 421 tctacattgt gcaggacctg atggagactg acctgtacaa gttgctgaaa aggccagcag 481 ctgagcaatg accataatct gctacttcct ctaccagatc ctgcggggcc tcaagtacat 541 ccactccgcc aacgtgctcc accgagatct aaagccctcc aacctgctca tcaacaccac 601 ntgcgacntt aagatttgtg atttcggcct ggcccggatt gccgatcctg agcatgacca 661 caccggcttc ctgacggagt atgtggctac gcgctggtac cgggccccag agatcatgct 721 gaactccaag ggctatacca agtccatcga catctggtct gtgggctgca ttctggctga 781 gatgctctct aaccggccca tcttccctgg caagcactac ctggatcagc tcaaccacat 841 tctgggcatc ctgggctccc catcccagga ggacctgaat tgtatcatca acatgaaggc 901 ccgaaactac ctacagtcTC TGCCCTCCAA GACCAAGGTG GCTTGGGCCA AGCTTTTCCC 961 CAAGTCAGAC TCCAAAGCCC TTGACCTGCT GGACCGGATG TTAACCTTTA ACCCCAATAA 1021 ACGGATCACA GTGGAGGAAG CGCTGGCTCA CCCCTACCTG GAGCAGTACT ATGACCCGAC 1081 GGATGAGCCA GTGGCCGAGG AGCCCTTCAC CTTCGCCATG GAGCTGGATG ACCTACCTAA 1141 GGAGCGGCTG AAGGAGCTCA TCTTCCAGGA GACAGCACGC TTCCAGCCCG GAGTGCTGGA 1201 GGCCCCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 1261 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 1321 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACAATAA GTATTACTCA AATACAAACG 1381 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt