Construct: ORF TRCN0000479612

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011351.1_s317c1
Derived from:
ccsbBroadEn_11007
DNA Barcode:
CTCAAACGATGGACCAATGACCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
OAZ1 (4946)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479612
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4946 OAZ1 ornithine decarboxylase ant... NM_004152.3 29.7% 100% 205_685del
2 human 4946 OAZ1 ornithine decarboxylase ant... NM_001301020.1 28.9% 97% 144_145insAGTTCC;199_679del
3 mouse 18245 Oaz1 ornithine decarboxylase ant... NM_008753.4 27.1% 89.7% (many diffs)
4 mouse 18245 Oaz1 ornithine decarboxylase ant... NM_001301034.1 26.3% 86.7% (many diffs)
5 mouse 100041965 Oaz1-ps ornithine decarboxylase ant... NR_027656.1 19.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
297
ORF length:
204
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGTGAA ATCCTCCCTG CAGCGGATCC
121TCAATAGCCA CTGCTTCGCC AGAGAGAAGG AAGGGGATAA ACCCAGCGCC ACCATCCACG
181CCAGCCGCAC CATGCCGCTC CTAAGCCTGC ACAGCCGCGG CGGCAGCAGC AGTGAGAGTT
241CCAGGGTCTC CCTCCACTGC TGTAGTAACC CGGGTCCGGG GCCTCGGTGG TGCTCCTTGC
301CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
361TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
421ATATATCTTG TGGAAAGGAC GACTCAAACG ATGGACCAAT GACCCCACGC GTTAAGTCga
481caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)