Construct: ORF TRCN0000479618
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001617.1_s317c1
- Derived from:
- ccsbBroadEn_05969
- DNA Barcode:
- CCACATGGACTGATTTATTGTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC5L (988)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479618
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 988 | CDC5L | cell division cycle 5 like | NM_001253.4 | 99.9% | 99.8% | 11T>G |
| 2 | mouse | 71702 | Cdc5l | cell division cycle 5-like ... | NM_152810.2 | 89.6% | 97.8% | (many diffs) |
| 3 | mouse | 71702 | Cdc5l | cell division cycle 5-like ... | XM_017317643.1 | 45.7% | 50.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2472
- ORF length:
- 2406
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcgaagtatg atcaaggggg gcgtatggag gaataccgag gatgaaattc 121 tgaaagcagc ggtaatgaaa tatgggaaaa atcagtggtc taggattgcc tcattgctgc 181 atagaaaatc agcaaagcag tgcaaagcca gatggtatga atggctggat ccaagcatta 241 agaagacaga atggtccaga gaagaagagg aaaaactctt gcacttggcc aagttgatgc 301 caactcagtg gaggaccatt gctccaatca ttggaagaac agcggcccag tgcttagaac 361 actatgaatt tcttctggat aaagctgccc aaagagacaa tgaagaggaa acaacagatg 421 atccacgaaa acttaaacct ggagaaatag atccaaatcc agaaacaaaa ccagcgcggc 481 ctgatccaat tgatatggat gaggatgaac ttgagatgct ttctgaagcc agagcccgct 541 tggctaatac tcagggaaag aaggccaaga ggaaagcaag agagaaacaa ttggaagaag 601 caagacgtct tgctgccctc caaaaaagaa gagaacttcg agcagctggc atagaaattc 661 agaagaaaag aaaaaggaag agaggagttg attataatgc cgaaatccca tttgaaaaaa 721 agcctgccct tggtttttat gatacttctg aggaaaacta ccaagctctt gacgcagatt 781 tcaggaaatt aagacaacag gatcttgatg gggagctaag atctgaaaaa gaaggaagag 841 atagaaaaaa agacaaacag catttgaaaa ggaaaaaaga atctgattta ccatcagcta 901 ttcttcaaac tagtggtgtt tctgaattta ctaaaaagag aagcaaacta gtacttcctg 961 cccctcagat ttcagatgca gaactccagg aagttgtaaa agtaggccaa gcgagtgaaa 1021 ttgcacgtca aactgccgag gaatctggca taacaaattc tgcttccagt acacttttgt 1081 ctgagtacaa tgtcaccaac aacagcgttg ctcttagaac accacgaaca ccagcttccc 1141 aggacagaat tctgcaggaa gcccagaacc tcatggccct caccaatgtg gacaccccat 1201 tgaaaggtgg acttaatacc ccattgcatg agagtgactt ctcaggtgta actccacagc 1261 gacaagttgt acagactcca aacacagttc tctctactcc attcaggact ccttctaatg 1321 gagctgaagg gctgactccc cggagtggaa caactcccaa accagttatt aactctactc 1381 cgggtagaac tcctcttcga gacaagttaa acattaatcc cgaggatgga atggcagact 1441 atagtgatcc ctcttacgtg aagcagatgg aaagagaatc ccgagaacat ctccgtttag 1501 ggttgttggg ccttcctgcc cctaagaatg attttgaaat tgttctacca gaaaatgccg 1561 agaaggagct ggaagaacgt gaaatagatg atacttacat tgaagatgct gctgatgtgg 1621 atgctcgaaa gcaggccata cgagatgcag agcgtgtaaa ggaaatgaaa cgaatgcata 1681 aagctgtcca gaaagatctg ccaagaccat cagaagtaaa tgaaactatt ctaagaccct 1741 taaatgtaga accgccttta acagatttac agaaaagtga agaactaatc aaaaaagaaa 1801 tgatcacaat gcttcattat gaccttctac atcaccctta tgaaccatct ggaaataaaa 1861 aaggcaaaac tgtagggttt ggtaccaata attcagagca cattacctat ctggaacata 1921 atccttatga aaagttctcc aaagaagagc tgaaaaaggc ccaggatgtt ttggtgcagg 1981 agatggaagt ggttaaacaa ggaatgagcc atggagagct ctcaagtgaa gcttataacc 2041 aggtgtggga agaatgctac agtcaagttt tatatcttcc tgggcagagc cgctacacac 2101 gggccaatct ggctagtaaa aaggacagaa ttgaaTCACT TGAAAAGAGG CTCGAGATAA 2161 ACAGGGGTCA CATGACGACA GAAGCCAAGA GGGCTGCAAA GATGGAAAAG AAGATGAAAA 2221 TTTTGCTTGG GGGTTACCAG TCTCGTGCTA TGGGGCTCAT GAAACAGTTG AATGACTTAT 2281 GGGACCAAAT TGAACAGGCT CACTTGGAGT TACGCACTTT TGAAGAACTC AAGAAACATG 2341 AAGATTCTGC TATTCCCCGG AGGCTAGAGT GTCTAAAAGA AGACGTTCAG CGACAACAAG 2401 AAAGAGAAAA GGAACTTCAA CATAGATATG CTGATTTGCT GCTGGAGAAA GAGACTTTAA 2461 AGTCAAAATT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2521 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2581 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCA CATGGACTGA TTTATTGTCT 2641 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t