Construct: ORF TRCN0000479634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017448.1_s317c1
- Derived from:
- ccsbBroadEn_04636
- DNA Barcode:
- TTGCCTTCCTCCCGTCTACTTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NACC1 (112939)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112939 | NACC1 | nucleus accumbens associated 1 | NM_052876.4 | 100% | 100% | |
| 2 | human | 112939 | NACC1 | nucleus accumbens associated 1 | XM_005259721.3 | 100% | 100% | |
| 3 | mouse | 66830 | Nacc1 | nucleus accumbens associate... | NM_025788.3 | 82.2% | 87.5% | (many diffs) |
| 4 | mouse | 66830 | Nacc1 | nucleus accumbens associate... | XM_006531292.2 | 82.2% | 87.5% | (many diffs) |
| 5 | mouse | 66830 | Nacc1 | nucleus accumbens associate... | XM_006531293.3 | 82.2% | 87.5% | (many diffs) |
| 6 | mouse | 66830 | Nacc1 | nucleus accumbens associate... | XM_006531294.3 | 82.2% | 87.5% | (many diffs) |
| 7 | mouse | 66830 | Nacc1 | nucleus accumbens associate... | XM_006531295.3 | 77.9% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1647
- ORF length:
- 1581
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccagacactg cagatggaga tcccgaactt cggcaacagc atcctggagt 121 gcctcaatga acagcggctg cagggcctgt actgtgacgt gtcagtggtg gtcaagggcc 181 atgccttcaa ggcccaccgg gccgtgcttg ctgccagcag ctcctacttc cgggacctgt 241 tcaacaacag ccgcagcgcc gtggtggagc tgccggcggc tgtgcagccc cagtctttcc 301 agcagatcct cagcttctgc tacacgggcc ggctgagcat gaacgtgggc gaccagttcc 361 tgctcatgta cacggctggc ttcctgcaga tccaggagat catggagaag ggcaccgagt 421 tcttcctcaa ggtgagctcc ccgagctgcg actcccaggg cctgcatgcg gaggaggccc 481 catcgtcgga gccccagagc cccgtggcgc agacatcggg ctggccagcc tgtagcaccc 541 cgctgcccct cgtgtcgcgg gtgaagacgg agcagcagga gtcggactcc gtgcagtgca 601 tgcccgtggc caagcggctg tgggacagtg gccagaagga ggctgggggc ggcggcaatg 661 gcagccgcaa gatggccaag ttctccacgc cggacctggc tgccaaccgg cctcaccagc 721 ccccgccacc ccaacaggct ccggtggtgg cagcagccca gcccgccgtg gctgcgggag 781 cagggcagcc agccggtggg gtggcagcag cagggggtgt ggtgagtggg cccagcacgt 841 cggagcggac cagcccaggc acctcaagcg cctacaccag cgacagccct ggctcctacc 901 acaatgagga ggacgaggag gaggatggtg gcgaggaggg catggatgag cagtaccggc 961 agatctgcaa catgtacacc atgtacagca tgatgaacgt cggccagaca gccgagaagg 1021 tggaggccct cccggagcag gtagcccccg agtcccgaaa tcgcatccgg gttcggcaag 1081 acctggcgtc tctcccggct gaacttatca accagattgg gaaccgctgc caccccaagc 1141 tctacgacga gggcgacccc tctgagaagc tggagctggt gacaggcacc aacgtgtaca 1201 tcacaagggc gcagctgatg aactgccacg tcagcgcagg cacgcggcac aaggtcctac 1261 tgcggcggct cctggcctcc ttctttgacc ggaacacgct ggccaacagc tgcggcaccg 1321 gcatccgctc ttctaccaac gaTCCCCGTC GGAAGCCCCT GGACAGCCGC GTGCTCCACG 1381 CTGTCAAGTA CTACTGCCAG AACTTCGCCC CCAACTTCAA GGAGAGCGAG ATGAATGCCA 1441 TCGCGGCCGA CATGTGCACC AACGCCCGCC GCGTCGTGCG CAAGAGCTGG ATGCCCAAGG 1501 TCAAGGTGCT CAAGGCTGAG GATGACGCCT ACACCACCTT CATCAGTGAA ACGGGCAAGA 1561 TCGAGCCGGA CATGATGGGT GTGGAGCATG GCTTCGAGAC CGCCAGCCAC GAGGGCGAGG 1621 CGGGTCCCTC GGCTGAAGCC CTGCAGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1681 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1741 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTGCCTTC 1801 CTCCCGTCTA CTTCGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1861 aagatt