Construct: ORF TRCN0000479644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013392.1_s317c1
- Derived from:
- ccsbBroadEn_11219
- DNA Barcode:
- AATGTTTAAATGAGGAGGAAAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BEST1 (7439)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7439 | BEST1 | bestrophin 1 | NM_001300786.2 | 99.9% | 100% | 1149G>A |
| 2 | human | 7439 | BEST1 | bestrophin 1 | NM_001300787.2 | 94.7% | 94.8% | 686_766del;1230G>A |
| 3 | human | 7439 | BEST1 | bestrophin 1 | XM_005274215.4 | 86% | 86% | 0_1ins138;548_628del;1092G>A |
| 4 | human | 7439 | BEST1 | bestrophin 1 | NM_004183.4 | 85% | 85.1% | 1_180del;866_946del;1410G>A |
| 5 | human | 7439 | BEST1 | bestrophin 1 | NM_001139443.2 | 82.1% | 81.7% | (many diffs) |
| 6 | human | 7439 | BEST1 | bestrophin 1 | XM_011545230.3 | 78.4% | 78% | (many diffs) |
| 7 | human | 7439 | BEST1 | bestrophin 1 | XM_005274210.4 | 74.7% | 74.3% | (many diffs) |
| 8 | human | 7439 | BEST1 | bestrophin 1 | XM_011545229.3 | 74.7% | 74.3% | (many diffs) |
| 9 | human | 7439 | BEST1 | bestrophin 1 | NM_001363591.2 | 74.5% | 74.1% | (many diffs) |
| 10 | human | 7439 | BEST1 | bestrophin 1 | NR_134580.2 | 61.8% | (many diffs) | |
| 11 | human | 7439 | BEST1 | bestrophin 1 | XR_001747952.2 | 54% | (many diffs) | |
| 12 | human | 7439 | BEST1 | bestrophin 1 | XM_011545233.3 | 50.5% | 47% | (many diffs) |
| 13 | human | 7439 | BEST1 | bestrophin 1 | XR_001747953.2 | 50.1% | (many diffs) | |
| 14 | human | 7439 | BEST1 | bestrophin 1 | XM_005274216.4 | 47.3% | 49.8% | 686_969del;1125_1126ins653 |
| 15 | human | 7439 | BEST1 | bestrophin 1 | XM_005274219.4 | 46.7% | 41% | 1_180del;865_866ins152;963_964ins559 |
| 16 | human | 7439 | BEST1 | bestrophin 1 | NM_001363593.2 | 44.9% | 44.5% | (many diffs) |
| 17 | human | 7439 | BEST1 | bestrophin 1 | XR_001747954.2 | 44.4% | (many diffs) | |
| 18 | human | 7439 | BEST1 | bestrophin 1 | NM_001363592.1 | 42.9% | 44.7% | 1_180del;866_1149del;1305_1306ins653 |
| 19 | human | 7439 | BEST1 | bestrophin 1 | XM_017018230.2 | 39.5% | 41.1% | 0_1ins138;548_831del;987_988ins653 |
| 20 | human | 7439 | BEST1 | bestrophin 1 | XM_005274221.4 | 37.6% | 32.5% | 1_180del;711_712ins305;810_811ins559 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1560
- ORF length:
- 1494
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgagaaactg actctgtatt gcgacagcta catccagctc atccccattt 121 ccttcgtgct gggcttctac gtgacgctgg tcgtgacccg ctggtggaac cagtacgaga 181 acctgccgtg gcccgaccgc ctcatgagcc tggtgtcggg cttcgtcgaa ggcaaggacg 241 agcaaggccg gctgctgcgg cgcacgctca tccgctacgc caacctgggc aacgtgctca 301 tcctgcgcag cgtcagcacc gcagtctaca agcgcttccc cagcgcccag cacctggtgc 361 aagcaggctt tatgactccg gcagaacaca agcagttgga gaaactgagc ctaccacaca 421 acatgttctg ggtgccctgg gtgtggtttg ccaacctgtc aatgaaggcg tggcttggag 481 gtcgaatccg ggaccctatc ctgctccaga gcctgctgaa cgagatgaac accttgcgta 541 ctcagtgtgg acacctgtat gcctacgact ggattagtat cccactggtg tatacacagg 601 tggtgactgt ggcggtgtac agcttcttcc tgacttgtct agttgggcgg cagtttctga 661 acccagccaa ggcctaccct ggccatgagc tggacctcgt tgtgcccgtc ttcacgttcc 721 tgcagttctt cttctatgtt ggctggctga aggtgtccct gttggctgtg gatgagatgc 781 accaggacct gcctcggatg gagccggaca tgtactggaa taagcccgag ccacagcccc 841 cctacacagc tgcttccgcc cagttccgtc gagcctcctt tatgggctcc accttcaaca 901 tcagcctgaa caaagaggag atggagttcc agcccaatca ggaggacgag gaggatgctc 961 acgctggcat cattggccgc ttcctaggcc tgcagtccca tgatcaccat cctcccaggg 1021 caaactcaag gaccaaacta ctgtggccca agagggaatc ccttctccac gagggcctgc 1081 ccaaaaacca caaggcagcc aaacagaacg ttaggggcca ggaagacaac aaggcctgga 1141 agcttaaggc tgtggacgcc ttcaagtctg ccccactgta tcagaggcca ggctactaca 1201 gtgccccaca gacaccccTC AGCCCCACTC CCATGTTCTT CCCCCTAGAA CCATCAGCGC 1261 CGTCAAAGCT TCACAGTGTC ACAGGCATAG ACACCAAAGA CAAAAGCTTA AAGACTGTGA 1321 GTTCTGGGGC CAAGAAAAGT TTTGAATTGC TCTCAGAGAG CGATGGGGCC TTGATGGAGC 1381 ACCCAGAAGT ATCTCAAGTG AGGAGGAAAA CTGTGGAGTT TAACCTGACG GATATGCCAG 1441 AGATCCCCGA AAATCACCTC AAAGAACCTT TGGAACAATC ACCAACCAAC ATACACACTA 1501 CACTCAAAGA TCACATGGAT CCTTATTGGG CCTTGGAAAA CAGGGATGAA GCACATTCCT 1561 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1621 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1681 TTTATATATC TTGTGGAAAG GACGAAATGT TTAAATGAGG AGGAAAATGA CGCGTTAAGT 1741 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt