Construct: ORF TRCN0000479658
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013709.1_s317c1
- Derived from:
- ccsbBroadEn_12884
- DNA Barcode:
- ACTTGTCGGCCGCATCTTTATAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C8orf76 (84933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479658
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100533106 | ZHX1-C8orf76 | ZHX1-C8orf76 readthrough | NM_001204180.2 | 100% | 100% | |
| 2 | human | 84933 | C8orf76 | chromosome 8 open reading f... | NM_032847.3 | 75.7% | 73% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct aaggaagctg tggcaatggt tttatgaaga aacagaaagc agtgatgatg 121 ttgaagtgct gactctcaag aaattcaaag gagacctggc ctacagacga caagagtatc 181 agaaagcact gcaggagtat tccagtatct ctgaaaaatt gtcatcaacc aattttgcca 241 tgaaaaggga tgtccaggaa ggtcaggctc ggtgtctggc tcacctgggt aggcatatgg 301 aggcgctgga gattgctgca aacttggaaa ataaagcaac caacacagac catttaacca 361 cggtactcta cctccagctt gctatttgtt caagtttgca gaacttggag aaaacaattt 421 tctgcctgca gaaactgatt tctttgcatc cttttaatcc ttggaactgg ggcaaattgg 481 cagaggctta cctgaatctg gggccagctc tttcagcagc acttgcgtca tcTCAGAAAC 541 AGCACAGTTT CACCTCAAGT GACAAAACTA TCAAATCCTT CTTTCCACAC TCAGGAAAAG 601 ACTGTCTTTT GTGTTTTCCT GAAACCTTGC CTGAGAGCTC TTTATTTTCT GTGGAAGCGA 661 ATAGCAGTAA TAGCCAGAAA AATGAGAAAG CTCTGACAAA TATCCAAAAC TGTATGGCAG 721 AAAAGAGAGA AACAGTGTTG ATAGAGACTC AGCTGAAAGC ATGTGCCTCT TTTATACGAA 781 CCAGGCTTCT GCTTCAGTTT ACCCAACCTC AGCAAACATC GTTTGCTTTG GAGAGGAACT 841 TAAGGACTCA GCAGGAAATT GAAGATAAAA TGAAAGGGTT CAGCTTCAAA GAAGACACTT 901 TGCTGTTGAT AGCTGAGGTG AGTGTATCCC TCGGGTTTAT GTGCCCAACT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGAACT TGTCGGCCGC ATCTTTATAG TACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t