Construct: ORF TRCN0000479659
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012532.1_s317c1
- Derived from:
- ccsbBroadEn_03926
- DNA Barcode:
- GGATCTGAGCGCCTTTTTGGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC39A8 (64116)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479659
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64116 | SLC39A8 | solute carrier family 39 me... | NM_001135146.2 | 100% | 100% | |
| 2 | human | 64116 | SLC39A8 | solute carrier family 39 me... | NM_022154.5 | 100% | 100% | |
| 3 | human | 64116 | SLC39A8 | solute carrier family 39 me... | XM_005263177.2 | 100% | 100% | |
| 4 | human | 64116 | SLC39A8 | solute carrier family 39 me... | NM_001135147.1 | 93.9% | 92.8% | (many diffs) |
| 5 | human | 64116 | SLC39A8 | solute carrier family 39 me... | XM_024454184.1 | 92.1% | 89.5% | (many diffs) |
| 6 | human | 64116 | SLC39A8 | solute carrier family 39 me... | NM_001135148.1 | 84.6% | 84.3% | 1_1delAins205;6_7insTT;11_15delACGCT |
| 7 | human | 64116 | SLC39A8 | solute carrier family 39 me... | XM_017008541.1 | 84.6% | 84.3% | 1_1delAins205;6_7insTT;11_15delACGCT |
| 8 | human | 64116 | SLC39A8 | solute carrier family 39 me... | XM_024454183.1 | 62.9% | 62.1% | (many diffs) |
| 9 | mouse | 67547 | Slc39a8 | solute carrier family 39 (m... | NM_001135149.1 | 83.8% | 88.9% | (many diffs) |
| 10 | mouse | 67547 | Slc39a8 | solute carrier family 39 (m... | NM_001135150.1 | 83.8% | 88.9% | (many diffs) |
| 11 | mouse | 67547 | Slc39a8 | solute carrier family 39 (m... | NM_026228.5 | 83.8% | 88.9% | (many diffs) |
| 12 | mouse | 67547 | Slc39a8 | solute carrier family 39 (m... | XM_006501934.2 | 83.8% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1446
- ORF length:
- 1380
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cccgggtcgc gcggtggccg ggctcctgtt gctggcggcc gccggcctcg 121 gaggagtggc ggaggggcca gggctagcct tcagcgagga tgtgctgagc gtgttcggcg 181 cgaatctgag cctgtcggcg gcgcagctcc agcacttgct ggagcagatg ggagccgcct 241 cccgcgtggg cgtcccggag cctggccagc tgcacttcaa ccagtgttta actgctgaag 301 agatcttttc ccttcatggc ttttcaaatg ctacccaaat aaccagctcc aaattctctg 361 tcatctgtcc agcagtctta cagcaattga actttcaccc atgtgaggat cggcccaagc 421 acaaaacaag accaagtcat tcagaagttt ggggatatgg attcctgtca gtgacgatta 481 ttaatctggc atctctcctc ggattgattt tgactccact gataaagaaa tcttatttcc 541 caaagatttt gacctttttt gtggggctgg ctattgggac tcttttttca aatgcaattt 601 tccaacttat tccagaggca tttggatttg atcccaaagt cgacagttat gttgagaagg 661 cagttgctgt gtttggtgga ttttacctac ttttcttttt tgaaagaatg ctaaagatgt 721 tattaaagac atatggtcag aatggtcata cccactttgg aaatgataac tttggtcctc 781 aagaaaaaac tcatcaacct aaagcattac ctgccatcaa tggtgtgaca tgctatgcaa 841 atcctgctgt cacagaagct aatggacata tccattttga taatgtcagt gtggtatctc 901 tacaggatgg aaaaaaagag ccaagttcat gtacctgttt gaaggggccc aaactgtcag 961 aaatagggac gattgcctgg atgataacgc tctgcgatgc cctccacaat ttcatcgatg 1021 gcctggcgat tggggcttcc tgcaccttgt ctctccttca gggactcagt acttccatag 1081 caaTCCTATG TGAGGAGTTT CCCCACGAGT TAGGAGACTT TGTGATCCTA CTCAATGCAG 1141 GGATGAGCAC TCGACAAGCC TTGCTATTCA ACTTCCTTTC TGCATGTTCC TGCTATGTTG 1201 GGCTAGCTTT TGGCATTTTG GTGGGCAACA ATTTCGCTCC AAATATTATA TTTGCACTTG 1261 CTGGAGGCAT GTTCCTCTAT ATTTCTCTGG CAGATATGTT TCCAGAGATG AATGATATGC 1321 TGAGAGAAAA GGTAACTGGA AGAAAAACCG ATTTCACCTT CTTCATGATT CAGAATGCTG 1381 GAATGTTAAC TGGATTCACA GCCATTCTAC TCATTACCTT GTATGCAGGA GAAATCGAAT 1441 TGGAGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1501 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1561 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGATCTGAG CGCCTTTTTG GCCTCACGCG 1621 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt