Construct: ORF TRCN0000479715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003944.1_s317c1
- Derived from:
- ccsbBroadEn_05035
- DNA Barcode:
- CGTACGGTCAGCTAATTACAACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIFM3 (150209)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479715
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150209 | AIFM3 | apoptosis inducing factor m... | NM_001018060.3 | 100% | 100% | |
| 2 | human | 150209 | AIFM3 | apoptosis inducing factor m... | NM_001146288.2 | 99% | 99% | 29_46del |
| 3 | human | 150209 | AIFM3 | apoptosis inducing factor m... | NM_144704.3 | 98.8% | 98.8% | 1757_1777del |
| 4 | human | 150209 | AIFM3 | apoptosis inducing factor m... | NR_027464.2 | 72.8% | 1_189del;434_435ins110;1874_2203del | |
| 5 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | NM_175178.4 | 87.9% | 96.1% | (many diffs) |
| 6 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | NM_001291070.1 | 86.9% | 95% | (many diffs) |
| 7 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_006522592.3 | 86.9% | 95% | (many diffs) |
| 8 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_006522590.3 | 85.2% | 92.1% | (many diffs) |
| 9 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_006522591.3 | 85.2% | 92.1% | (many diffs) |
| 10 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_006522593.3 | 83% | 90% | (many diffs) |
| 11 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_006522594.3 | 80% | 84.7% | (many diffs) |
| 12 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_006522595.3 | 78.7% | 85.2% | (many diffs) |
| 13 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_006522596.3 | 78.7% | 85.2% | (many diffs) |
| 14 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_017317135.1 | 77.5% | 84.1% | (many diffs) |
| 15 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XM_017317136.1 | 73.4% | 79.6% | (many diffs) |
| 16 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XR_001781867.1 | 41.4% | (many diffs) | |
| 17 | mouse | 72168 | Aifm3 | apoptosis-inducing factor, ... | XR_001781868.1 | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1860
- ORF length:
- 1794
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cggctgcttc tccaaaccca aaccagtgga gctcaagatc gaggtggtgc 121 tgcctgagaa ggagcgaggc aaggaggagc tgtcggccag tgggaagggc agcccccggg 181 cctaccaggg caatggcacg gcccgccact tccacacgga ggagcgcctg tccacccctc 241 acccctaccc cagccctcag gattgcgtgg aggctgctgt ctgccacgtc aaggacctcg 301 agaatggcca gatgcgggaa gtggagctgg gctgggggaa ggtgttgctg gtgaaggaca 361 atggggagtt ccacgccctg ggccataagt gtccgcacta cggcgcaccc ctggtgaaag 421 gcgttctgtc ccgtggtcgg gtgcgctgcc cctggcacgg cgcctgcttc aacatcagca 481 ctggggacct ggaggacttc cctggcctgg acagtctaca caagttccag gtgaagattg 541 agaaggagaa ggtgtacgtc cgggccagca agcaggccct acagctgcag cgaaggacca 601 aggtgatggc caagtgtatc tctccaagtg ctgggtacag cagtagcacc aatgtgctca 661 ttgtgggtgc aggtgcagct ggcctggtgt gtgcagagac actgcggcag gagggcttct 721 ccgaccggat cgtcctgtgc acgctagacc ggcaccttcc ctacgaccgt cccaagctca 781 gcaagtccct ggacacacag cctgagcagc tggccctgag gcccaaggag tttttccgag 841 cctatggcat cgaggtgctc accgaggctc aggtggtcac agtggacgtg agaactaaga 901 aggtcgtgtt caaggatggc ttcaagctgg agtacagcaa gctgctgctg gcaccaggga 961 gcagccccaa gactctgagc tgcaaaggca aagaagtgga gaacgtgttc actatccgga 1021 cgccagagga tgccaatcgc gtggtgaggc tggcccgagg ccgcaacgtg gtcgtcgtgg 1081 gagccggctt cctggggatg gaggtggccg cttacctgac ggagaaggcc cactctgtgt 1141 ctgtggtgga gctggaggag acgcccttca ggaggttcct gggggagcgc gtgggtcgtg 1201 ccctcatgaa gatgtttgag aacaaccggg tgaagttcta catgcagacg gaggtgtctg 1261 agctgcgggg ccaggaggga aagctgaagg aggttgtgct gaagagcagc aaggtcgtgc 1321 gggctgacgt ctgcgtggtg ggcattggtg cagtgcccgc cacaggcttc ctgaggcaaa 1381 gcggcatcgg tttggattcc cgaggcttca tccctgtcaa caagatgatg cagaccaatg 1441 tcccaggcgt gtttgcagct ggcgatgctg tcaccttccc ccttgcctgg aggaacaacc 1501 gcaaagtgaa cattccacat tggcagatgg ctcatgcTCA GGGGCGCGTG GCAGCCCAGA 1561 ACATGTTGGC GCAGGAGGCG GAGATGAGCA CTGTGCCCTA CCTCTGGACC GCCATGTTTG 1621 GCAAGAGCCT GCGCTACGCG GGCTACGGAG AAGGCTTCGA CGACGTCATC ATCCAGGGGG 1681 ATCTGGAGGA GCTGAAGTTT GTGGCTTTTT ACACTAAAGG CGACGAGGTG ATCGCCGTGG 1741 CCAGCATGAA CTACGATCCC ATTGTGTCCA AGGTCGCTGA GGTGCTGGCC TCAGGCCGTG 1801 CCATCCGGAA GCGGGAGGTG GAGACTGGCG ACATGTCCTG GCTTACGGGG AAAGGATCCT 1861 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1921 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1981 TTTATATATC TTGTGGAAAG GACGACGTAC GGTCAGCTAA TTACAACCCA CGCGTTAAGT 2041 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt