Construct: ORF TRCN0000479728
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015455.1_s317c1
- Derived from:
- ccsbBroadEn_11500
- DNA Barcode:
- CATTACGGAAGAAAATTAACAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECI2 (10455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479728
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | NM_001166010.1 | 98.6% | 98.6% | 1_15del |
| 2 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | NM_006117.2 | 98.6% | 98.6% | 1_15del |
| 3 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | NM_206836.2 | 91.1% | 91.1% | 1_105del |
| 4 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | XM_017010170.1 | 90.3% | 90.3% | 1_15del;704_705ins90 |
| 5 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | XM_006714957.2 | 83.5% | 83.5% | 1_105del;794_795ins90 |
| 6 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | NR_028588.1 | 72.7% | 1_132del;527_597del;1281_1481del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agccagtcag aaggactttg aaaattcaat gaatcaagtg aaactcttga 121 aaaaggatcc aggaaacgaa gtgaagctaa aactctacgc gctatataag caggccactg 181 aaggaccttg taacatgccc aaaccaggtg tatttgactt gatcaacaag gccaaatggg 241 acgcatggaa tgcccttggc agcctgccca aggaagctgc caggcagaac tatgtggatt 301 tggtgtccag tttgagtcct tcattggaat cctctagtca ggtggagcct ggaacagaca 361 ggaaatcaac tgggtttgaa actctggtgg tgacctccga agatggcatc acaaagatca 421 tgttcaaccg gcccaaaaag aaaaatgcca taaacactga gatgtatcat gaaattatgc 481 gtgcacttaa agctgccagc aaggatgact caatcatcac tgttttaaca ggaaatggtg 541 actattacag tagtgggaat gatctgacta acttcactga tattccccct ggtggagtag 601 aggagaaagc taaaaataat gccgttttac tgagggaatt tgtgggctgt tttatagatt 661 ttcctaagcc tctgattgca gtggtcaatg gtccagctgt gggcatctcc gtcaccctcc 721 ttgggctatt cgatgccgtg tatgcatctg acagggcaac atttcataca ccatttagtc 781 accTAGGCCA AAGTCCGGAA GGATGCTCCT CTTACACTTT TCCGAAGATA ATGAGCCCAG 841 CCAAGGCAAC AGAGATGCTT ATTTTTGGAA AGAAGTTAAC AGCGGGAGAG GCATGTGCTC 901 AAGGACTTGT TACTGAAGTT TTCCCTGATA GCACTTTTCA GAAAGAAGTC TGGACCAGGC 961 TGAAGGCATT TGCAAAGCTT CCCCCAAATG CCTTGAGAAT TTCAAAAGAG GTAATCAGGA 1021 AAAGAGAGAG AGAAAAACTA CACGCTGTTA ATGCTGAAGA ATGCAATGTC CTTCAGGGAA 1081 GATGGCTATC AGATGAATGC ACAAATGCTG TGGTGAACTT CTTATCCAGA AAATCAAAAC 1141 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGACA TTACGGAAGA AAATTAACAG TGACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt