Construct: ORF TRCN0000479730
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013197.1_s317c1
- Derived from:
- ccsbBroadEn_01446
- DNA Barcode:
- GCTGACGAATGTTAATAATTAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS3A (6189)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479730
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6189 | RPS3A | ribosomal protein S3A | NM_001006.5 | 100% | 100% | |
| 2 | human | 6189 | RPS3A | ribosomal protein S3A | NM_001267699.1 | 21.7% | 21.2% | (many diffs) |
| 3 | mouse | 20091 | Rps3a1 | ribosomal protein S3A1 | NM_016959.4 | 89.2% | 99.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggttggcaag aacaagcgcc ttacgaaagg cggcaaaaag ggagccaaga 121 agaaagtggt tgatccattt tctaagaaag attggtatga tgtgaaagca cctgctatgt 181 tcaatataag aaatattgga aagacgctcg tcaccaggac ccaaggaacc aaaattgcat 241 ctgatggtct caagggtcgt gtgtttgaag tgagtcttgc tgatttgcag aatgatgaag 301 ttgcatttag aaaattcaag ctgattactg aagatgttca gggtaaaaac tgcctgacta 361 acttccatgg catggatctt acccgtgaca aaatgtgttc catggtcaaa aaatggcaga 421 caatgattGA AGCTCACGTT GATGTCAAGA CTACCGATGG TTACTTGCTT CGTCTGTTCT 481 GTGTTGGTTT TACTAAAAAA CGCAACAATC AGATACGGAA GACCTCTTAT GCTCAGCACC 541 AACAGGTCCG CCAAATCCGG AAGAAGATGA TGGAAATCAT GACCCGAGAG GTGCAGACAA 601 ATGACTTGAA AGAAGTGGTC AATAAATTGA TTCCAGACAG CATTGGAAAA GACATAGAAA 661 AGGCTTGCCA ATCTATTTAT CCTCTCCATG ATGTCTTCGT TAGAAAAGTA AAAATGCTGA 721 AGAAGCCCAA GTTTGAATTG GGAAAGCTCA TGGAGCTTCA TGGTGAAGGC AGTAGTTCTG 781 GAAAAGCCAC TGGGGACGAG ACAGGTGCTA AAGTTGAACG AGCTGATGGA TATGAACCAC 841 CAGTCCAAGA ATCTGTTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCTGACG AATGTTAATA 1021 ATTAATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt