Construct: ORF TRCN0000479772

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012390.1_s317c1
Derived from:
ccsbBroadEn_09342
DNA Barcode:
AGGCAATCGTACGATGCCATACCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FANK1 (92565)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479772
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92565 FANK1 fibronectin type III and an... NM_145235.5 99.9% 99.7% 14A>G
2 human 92565 FANK1 fibronectin type III and an... NM_001363549.1 98.2% 98.2% 0_1insATGGAGCCCCAGAGAATC
3 human 92565 FANK1 fibronectin type III and an... XM_017016921.2 98.2% 98.2% 0_1insATGGAGCCCCAGAGAATC
4 human 92565 FANK1 fibronectin type III and an... XM_024448252.1 96.8% 95.7% (many diffs)
5 human 92565 FANK1 fibronectin type III and an... XM_006718064.3 96.5% 96.2% 0_1insATGGAGCCCCAGAGAATC;299_316del
6 human 92565 FANK1 fibronectin type III and an... XM_024448253.1 96.5% 96.2% 0_1insATGGAGCCCCAGAGAATC;299_316del
7 human 92565 FANK1 fibronectin type III and an... XM_006718058.4 95.2% 93.9% (many diffs)
8 human 92565 FANK1 fibronectin type III and an... NM_001350939.1 92.9% 92.7% 14A>G;317_394del
9 human 92565 FANK1 fibronectin type III and an... XM_011540349.3 91.9% 91.3% (many diffs)
10 human 92565 FANK1 fibronectin type III and an... XM_024448258.1 91.8% 91.8% 0_1insATGGAGCCCCAGAGAATC;452_453ins66
11 human 92565 FANK1 fibronectin type III and an... XM_011540351.2 91.3% 91.3% 0_1insATGGAGCCCCAGAGAATC;299_376del
12 human 92565 FANK1 fibronectin type III and an... XM_017016917.2 91.3% 91.3% 0_1insATGGAGCCCCAGAGAATC;299_376del
13 human 92565 FANK1 fibronectin type III and an... XM_017016918.1 91.3% 91.3% 0_1insATGGAGCCCCAGAGAATC;299_376del
14 human 92565 FANK1 fibronectin type III and an... XM_017016919.1 91.3% 91.3% 0_1insATGGAGCCCCAGAGAATC;299_376del
15 human 92565 FANK1 fibronectin type III and an... XM_024448256.1 90.3% 90% 0_1insATGGAGCCCCAGAGAATC;299_316del;470_471ins66
16 human 92565 FANK1 fibronectin type III and an... XM_024448257.1 90.3% 90% 0_1insATGGAGCCCCAGAGAATC;299_316del;470_471ins66
17 human 92565 FANK1 fibronectin type III and an... XM_011540348.2 90.2% 89.2% (many diffs)
18 human 92565 FANK1 fibronectin type III and an... XM_024448251.1 90.2% 89.2% (many diffs)
19 human 92565 FANK1 fibronectin type III and an... XM_017016923.2 88% 87.8% 14A>G;848_849ins123
20 human 92565 FANK1 fibronectin type III and an... XM_011540358.3 85.2% 84.2% (many diffs)
21 human 92565 FANK1 fibronectin type III and an... XM_017016920.2 84.4% 83.4% (many diffs)
22 human 92565 FANK1 fibronectin type III and an... XM_024448254.1 79.4% 78.4% (many diffs)
23 human 92565 FANK1 fibronectin type III and an... XM_017016922.2 76.9% 76% (many diffs)
24 human 92565 FANK1 fibronectin type III and an... XM_011540360.3 71.9% 70.2% (many diffs)
25 human 92565 FANK1 fibronectin type III and an... XM_011540361.2 71.9% 70.2% (many diffs)
26 human 92565 FANK1 fibronectin type III and an... XM_017016924.1 71.9% 70.2% (many diffs)
27 human 92565 FANK1 fibronectin type III and an... XM_005270280.4 57.1% 57.1% 0_1ins444
28 human 92565 FANK1 fibronectin type III and an... XM_011540362.3 57.1% 57.1% 0_1ins444
29 human 92565 FANK1 fibronectin type III and an... XM_017016926.1 57.1% 57.1% 0_1ins444
30 human 92565 FANK1 fibronectin type III and an... XR_945878.3 50.6% (many diffs)
31 human 92565 FANK1 fibronectin type III and an... XM_011540363.3 41.1% 40.1% (many diffs)
32 mouse 66930 Fank1 fibronectin type 3 and anky... NM_025850.2 85.7% 88.1% (many diffs)
33 mouse 66930 Fank1 fibronectin type 3 and anky... XM_006508120.2 79.8% 81.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1101
ORF length:
1035
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gccccagaga atcatgccac cctcaaagcc tcatccacct gtcgtgggca
121aagtgactca tcacagcatt gaattatact gggatctgga aaagaaagcc aaacgccaag
181gacctcaaga gcagtggttc aggttctcga ttgaagaaga agaccccaaa atgcacactt
241atggtatcat ttatacggga tatgcaacga agcatgttgt tgaaggtctg gaaccaagga
301cgctgtacag atttcgcctg aaggtcacca gcccctctgg ggagtgtgag tacagcccac
361tcgtctcagt gtctacaacc agagagccca taagtagtga gcacttgcac cgggctgtca
421gtgtgaatga tgaagatttg ctggtccgaa tacttcaagg aggccgtgtt aaggttgatg
481ttcccaataa gtttggcttt accgctctga tggttgctgc ccagaaagga tacaccaggc
541ttgtgaaaat cctagtttct aatggcacag acgtgaatct gaagaatgga agtggcaagg
601acagtctaat gctggcgtgc tatgcgggac acctagatgt tgtgaaatat ctccgaagac
661atggcgcttc ttggcaggct agagacctgg gaggctgtac agctctgcac tgggctgcag
721atggaggcca ctgcagtgtg attgagtgGA TGATAAAGGA TGGCTGTGAG GTAGACGTCG
781TGGACACTGG TTCAGGATGG ACCCCACTCA TGAGAGTCTC TGCGGTGTCG GGAAATCAGA
841GGGTGGCCTC TCTTCTAATT GATGCTGGGG CCAATGTGAA TGTGAAGGAC AGAAATGGAA
901AGACGCCCCT TATGGTGGCT GTGTTAAATA ATCATGAAGA GTTAGTTCAG TTACTTCTTG
961ACAAAGGGGC AGATGCAAGT GTAAAAAATG AGTTCGGCAA AGGTGTCCTA GAAATGGCCA
1021GAGTTTTTGA CAGACAGAGT GTAGTCTCCT TATTAGAAGA AAGGAAAAAA AAGCAGAGGC
1081CAAAGAAGTC TTGTGTCTGC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1141CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1201AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGGC AATCGTACGA
1261TGCCATACCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)