Construct: ORF TRCN0000479778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002409.2_s317c1
- Derived from:
- ccsbBroadEn_12815
- DNA Barcode:
- TTATACGTGCAAGCCCCTCCCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKT1S1 (84335)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479778
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84335 | AKT1S1 | AKT1 substrate 1 | NM_001098632.2 | 49.2% | 49.2% | 1_390del |
| 2 | human | 84335 | AKT1S1 | AKT1 substrate 1 | NM_001098633.4 | 49.2% | 49.2% | 1_390del |
| 3 | human | 84335 | AKT1S1 | AKT1 substrate 1 | NM_001278159.1 | 49.2% | 49.2% | 1_390del |
| 4 | human | 84335 | AKT1S1 | AKT1 substrate 1 | NM_001278160.1 | 49.2% | 49.2% | 1_390del |
| 5 | human | 84335 | AKT1S1 | AKT1 substrate 1 | NM_032375.5 | 45.6% | 45.6% | 1_450del |
| 6 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | NM_001290695.1 | 71% | 76.9% | (many diffs) |
| 7 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | NM_001253920.1 | 43.2% | 46.6% | (many diffs) |
| 8 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | NM_026270.4 | 43.2% | 46.6% | (many diffs) |
| 9 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | XM_006541119.3 | 43.2% | 46.6% | (many diffs) |
| 10 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | XM_006541120.3 | 43.2% | 46.6% | (many diffs) |
| 11 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | XM_006541121.3 | 43.2% | 46.6% | (many diffs) |
| 12 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | XM_006541122.2 | 43.2% | 46.6% | (many diffs) |
| 13 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | XM_006541118.3 | 42.7% | 46.1% | (many diffs) |
| 14 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | XM_006541117.1 | 42% | 45.4% | (many diffs) |
| 15 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | NM_001290694.1 | 39.2% | 42.4% | (many diffs) |
| 16 | mouse | 67605 | Akt1s1 | AKT1 substrate 1 (proline-r... | XM_006541115.3 | 33.8% | 36.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 444
- ORF length:
- 378
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgaggacgcc accctccagg accttccccc cTTCTGTGAG TCAGACCCCG 121 AGAGTACAGA TGATGGCAGC CTGAGCGAGG AGACCCCCGC CGGCCCCCCC ACCTGCTCAG 181 TGCCCCCAGC CTCAGCCCTA CCCACACAGC AGTACGCCAA GTCCCTGCCT GTGTCTGTGC 241 CCGTCTGGGG CTTCAAGGAG AAGAGGACAG AGGCGCGGTC ATCAGATGAG GAGAATGGGC 301 CGCCCTCTTC GCCCGACCTG GACCGCATCG CGGCGAGCAT GCGCGCGCTG GTGCTGCGAG 361 AGGCCGAGGA CACCCAGGTC TTCGGGGACC TGCCACGGCC GCGGCTTAAC ACCAGCGACT 421 TCCAGAAGCT GAAGCGGAAA TATTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TATACGTGCA 601 AGCCCCTCCC CAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att