Construct: ORF TRCN0000479785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010248.1_s317c1
- Derived from:
- ccsbBroadEn_05765
- DNA Barcode:
- GTCCCCTGGATCAGGACGGTAAAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ACTG2 (72)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479785
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001615.4 | 99.7% | 6T>A;354A>G;1121A>C | |
| 2 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001199893.2 | 88.2% | (many diffs) | |
| 3 | human | 70 | ACTC1 | actin alpha cardiac muscle 1 | NM_005159.5 | 85.8% | (many diffs) | |
| 4 | mouse | 11468 | Actg2 | actin, gamma 2, smooth musc... | NM_009610.2 | 92.7% | (many diffs) | |
| 5 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_001272041.1 | 85.7% | (many diffs) | |
| 6 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_009606.3 | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 69
- ORF length:
- 3
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg agaagaggag accaccgcgc tcgtgtgtga caatggctct ggcctgtgca 121 aggcaggctt cgcaggagat gatgcccccc gggctgtctt cccctccatt gtgggccgcc 181 ctcgccacca gggtgtgatg gtgggaatgg gccagaaaga cagctatgtg ggggatgagg 241 ctcagagcaa gcgagggatc ctaactctca aataccccat tgaacacggc atcatcacca 301 actgggatga catggagaag atctggcacc actccttcta caatgagctg cgtgtagcac 361 ctgaagagca ccccaccctg ctcacagagg ctcccctaaa tcccaaggcc aacagggaga 421 agatgaccca gatcatgttt gaaaccttca atgtccctgc catgtacgtc gccattcaag 481 ctgtgctctc cctctatgcc tctggccgca cgacaggcat cgtcctggat tcaggtgatg 541 gcgtcaccca caatgtcccc atctatgaag gctatgccct gccccatgcc atcatgcgcc 601 tggacttggc tggccgtgac ctcacggact acctcatgaa gatcctcaca gagagaggct 661 attcctttgt gaccacagct gagagagaaa ttgtgcgaga catcaaggag aagctgtgct 721 atgtggccct ggattttgag aatgagatgg ccacagcagc ttcctcttcc tccctggaga 781 agagctatga gctgccagat gggcaggtta tcaccattgg caatgagcgc ttccgctgcc 841 cTGAGACCCT CTTCCAGCCT TCCTTTATTG GCATGGAGTC CGCTGGAATT CATGAGACAA 901 CCTACAATTC CATCATGAAG TGTGACATTG ACATCCGTAA GGACTTATAT GCCAACAATG 961 TCCTCTCTGG GGGCACCACC ATGTACCCTG GCATTGCTGA CAGGATGCAG AAGGAGATCA 1021 CAGCCCTGGC CCCCAGCACC ATGAAGATCA AGATTATTGC TCCCCCAGAG CGGAAGTACT 1081 CAGTCTGGAT CGGGGGCTCT ATCCTGGCCT CTCTCTCCAC CTTCCAGCAG ATGTGGATCA 1141 GCAAGCCTGA GTATGATGAG GCAGGGCCCT CCATTGTCCA CAGGACGTGC TTCTACCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAG TCCCCTGGAT CAGGACGGTA AAAACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att