Construct: ORF TRCN0000479793
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000710.1_s317c1
- Derived from:
- ccsbBroadEn_10710
- DNA Barcode:
- ATACAAGAACATCTTCTGCATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASP8 (841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479793
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 841 | CASP8 | caspase 8 | NM_033358.4 | 81.2% | 69.1% | (many diffs) |
| 2 | human | 841 | CASP8 | caspase 8 | XM_006712793.3 | 81.2% | 69.1% | (many diffs) |
| 3 | human | 841 | CASP8 | caspase 8 | XM_005246893.3 | 67.1% | 57.1% | (many diffs) |
| 4 | human | 841 | CASP8 | caspase 8 | XM_005246895.3 | 65.2% | 56.3% | 1_177del;725_726ins65;837_838ins109 |
| 5 | human | 841 | CASP8 | caspase 8 | XR_923035.1 | 63.9% | (many diffs) | |
| 6 | human | 841 | CASP8 | caspase 8 | XR_001738971.1 | 60% | (many diffs) | |
| 7 | human | 841 | CASP8 | caspase 8 | NM_001080124.2 | 58.5% | 52.6% | (many diffs) |
| 8 | human | 841 | CASP8 | caspase 8 | NM_033356.4 | 58.5% | 52.6% | (many diffs) |
| 9 | human | 841 | CASP8 | caspase 8 | XM_005246892.2 | 58.5% | 52.6% | (many diffs) |
| 10 | human | 841 | CASP8 | caspase 8 | NM_001372051.1 | 56.7% | 51.1% | (many diffs) |
| 11 | human | 841 | CASP8 | caspase 8 | NM_033355.3 | 56.7% | 51.1% | (many diffs) |
| 12 | human | 841 | CASP8 | caspase 8 | XM_005246887.2 | 56.7% | 51.1% | (many diffs) |
| 13 | human | 841 | CASP8 | caspase 8 | XM_005246888.2 | 56.7% | 51.1% | (many diffs) |
| 14 | human | 841 | CASP8 | caspase 8 | XM_005246889.2 | 56.7% | 51.1% | (many diffs) |
| 15 | human | 841 | CASP8 | caspase 8 | XM_005246890.4 | 56.7% | 51.1% | (many diffs) |
| 16 | human | 841 | CASP8 | caspase 8 | XM_005246891.5 | 56.7% | 51.1% | (many diffs) |
| 17 | human | 841 | CASP8 | caspase 8 | XM_006712789.2 | 56.7% | 51.1% | (many diffs) |
| 18 | human | 841 | CASP8 | caspase 8 | XM_006712790.4 | 56.7% | 51.1% | (many diffs) |
| 19 | human | 841 | CASP8 | caspase 8 | NM_001228.4 | 54.8% | 49.4% | (many diffs) |
| 20 | human | 841 | CASP8 | caspase 8 | XM_005246885.2 | 51.9% | 46.9% | (many diffs) |
| 21 | human | 841 | CASP8 | caspase 8 | NM_001080125.2 | 50.5% | 45.6% | (many diffs) |
| 22 | human | 841 | CASP8 | caspase 8 | XM_011511969.2 | 26.4% | 21.9% | (many diffs) |
| 23 | human | 841 | CASP8 | caspase 8 | NR_111983.2 | 26.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 903
- ORF length:
- 834
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacttcagc agaaatcttt atgatattgg ggaacaactg gacagtgaag 121 atctggcctc cctcaagttc ctgagcctgg actacattcc gcaaaggaag caagaaccca 181 tcaaggatgc cttgatgtta ttccagagac tccaggaaaa gagaatgttg gaggaaagca 241 atctgtcctt cctgaaggag ctgctcttcc gaattaatag actggatttg ctgattacct 301 acctaaacac tagaaaggag gagatggaaa gggaacttca gacaccaggc agggctcaaa 361 tttctgccta cagggtcatg ctctatcaga tttcagaaga agtgagcaga tcagaattga 421 ggtcttttaa gtttcttttg caagaggaaa tctccaaatg caaactggat gatgacatga 481 acctgctgga tattttcata gagatggaga agagggtcat cctgggagaa ggaaagttgg 541 acatcctgaa aagagtctgt gcccaaatca acaagagcct gctgaagata atCAACGACT 601 ATGAAGAATT CAGCAAAGGG GAGGAGTTGT GTGGGGTAAT GACAATCTCG GACTCTCCAA 661 GAGAACAGGA TAGTGAATCA CAGACTTTGG ACAAAGTTTA CCAAATGAAA AGCAAACCTC 721 GGGGATACTG TCTGATCATC AACAATCACA ATTTTGCAAA AGCACGGGAG AAAGTGCCCA 781 AACTTCACAG CATTAGGGAC AGGAATGGAA CACACTTGGA TGCAGGGTCT CACTCTGTTG 841 CCCAGGCTGG AGTGCAGTGG TGTGATCTCG GCTCACTGCA GCCTCCGCCT CCCTGGTTCG 901 GGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGAAT ACAAGAACAT CTTCTGCATT CAACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt