Construct: ORF TRCN0000479812
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015007.1_s317c1
- Derived from:
- ccsbBroadEn_04806
- DNA Barcode:
- CGCATTCCAGTCTCCTTAGGTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCP2 (126006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479812
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126006 | PCP2 | Purkinje cell protein 2 | NM_174895.3 | 100% | 100% | |
| 2 | human | 126006 | PCP2 | Purkinje cell protein 2 | XM_017026251.2 | 100% | 100% | |
| 3 | human | 126006 | PCP2 | Purkinje cell protein 2 | NM_001271830.2 | 87.7% | 87.5% | 0_1ins49;2delT |
| 4 | human | 126006 | PCP2 | Purkinje cell protein 2 | XM_006722639.3 | 72.7% | 72.7% | 0_1ins111 |
| 5 | human | 126006 | PCP2 | Purkinje cell protein 2 | XM_017026252.2 | 72.7% | 72.7% | 0_1ins111 |
| 6 | human | 126006 | PCP2 | Purkinje cell protein 2 | XM_017026249.2 | 53.9% | 47.8% | 1_163del;214_398del |
| 7 | human | 126006 | PCP2 | Purkinje cell protein 2 | XM_017026250.2 | 53.9% | 47.8% | 1_163del;214_398del |
| 8 | human | 126006 | PCP2 | Purkinje cell protein 2 | XM_024451346.1 | 53.9% | 47.8% | 1_163del;214_398del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatggatcag gaggagaaga cggaggaagg ctcaggcccc tgtgccgagg 121 cgggctcccc agaccaggag ggcttcTTCA ATCTGCTGAG CCACGTGCAG GGCGACCGGA 181 TGGAGGGACA GCGCTGTTCA CTGCAAGCCG GGCCGGGCCA GACCACCAAG AGCCAGAGCG 241 ACCCCACCCC CGAGATGGAC AGCCTCATGG ACATGCTGGC CAGTACCCAG GGCCGCCGCA 301 TGGATGACCA ACGTGTGACA GTCAGCAGCC TGCCCGGCTT CCAGCCCGTG GGGTCCAAGG 361 ACGGAGCACA GAAACGAGCT GGGACCCTCA GTCCCCAACC CCTGCTCACC CCTCAGGACC 421 CGACCGCTCT CGGCTTCCGT CGGAACAGCA GCCCCCAGCC CCCGACACAA GCCCCCTTGC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GACGCATTCC AGTCTCCTTA GGTTACACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt