Construct: ORF TRCN0000479824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001850.1_s317c1
- Derived from:
- ccsbBroadEn_11632
- DNA Barcode:
- AGACATCCGGGACGTTCCACGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COBLL1 (22837)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001278461.2 | 8% | 6.9% | (many diffs) |
| 2 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001365672.2 | 8% | 6.9% | (many diffs) |
| 3 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001365673.2 | 8% | 6.9% | (many diffs) |
| 4 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001365674.2 | 7.9% | 6.9% | (many diffs) |
| 5 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001365675.2 | 7.9% | 6.9% | (many diffs) |
| 6 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001365671.1 | 7.8% | 6.7% | (many diffs) |
| 7 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_014900.5 | 7.8% | 6.7% | (many diffs) |
| 8 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001365670.2 | 7.7% | 6.7% | (many diffs) |
| 9 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001278460.2 | 7.7% | 6.7% | (many diffs) |
| 10 | human | 22837 | COBLL1 | cordon-bleu WH2 repeat prot... | NM_001278458.2 | 7.3% | 6.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 408
- ORF length:
- 318
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGACGGCCG AACCCCGCGC CCGCAGGACG 121 CCCCAGCCAG GAGAAAACCA AAAGCCAAGG CACCACTTCC TCCAGCTGAG ACCAAATATA 181 CTGATGTCTC TTCAGCTGCT GATTCTGTAG AATCCACTGC TTTCATCATG GAACAGAAAG 241 AAAACATGAT AGATAAAGAC GTTGAACTCT CAGTGGTCCT ACCTGGGGAT ATTATCAAAT 301 CTACTACTGT TCATGGCAGG TACGCTGGAG TAAATGAAAT ATCAGGACCT CCCCTTCTTA 361 TTCCACCATT GAAAGTCTCT GTGATATACG TCTTGATGTT TGTTCTTTGC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGAAGACATC CGGGACGTTC CACGCTCACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt