Construct: ORF TRCN0000479827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016449.1_s317c1
- Derived from:
- ccsbBroadEn_04043
- DNA Barcode:
- CCTACAGCTACGCAATGAGCTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MLPH (79083)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79083 | MLPH | melanophilin | NM_024101.7 | 100% | 100% | |
| 2 | human | 79083 | MLPH | melanophilin | XM_017004893.1 | 99.3% | 98% | (many diffs) |
| 3 | human | 79083 | MLPH | melanophilin | NM_001042467.3 | 95.3% | 95.3% | 1018_1019ins84 |
| 4 | human | 79083 | MLPH | melanophilin | XM_006712737.1 | 93.3% | 93.3% | 555_556ins120 |
| 5 | human | 79083 | MLPH | melanophilin | XM_006712739.1 | 91.3% | 91.3% | 1287_1288ins156 |
| 6 | human | 79083 | MLPH | melanophilin | XM_006712740.1 | 88.6% | 88.6% | 555_556ins120;898_899ins84 |
| 7 | human | 79083 | MLPH | melanophilin | XM_017004894.2 | 83.7% | 82.3% | (many diffs) |
| 8 | human | 79083 | MLPH | melanophilin | NM_001281473.2 | 80% | 80% | 555_556ins120;898_899ins84;1083_1084ins156 |
| 9 | human | 79083 | MLPH | melanophilin | NM_001281474.2 | 76.1% | 76.1% | 673_674ins429 |
| 10 | human | 79083 | MLPH | melanophilin | XM_011511812.1 | 75.6% | 75.1% | (many diffs) |
| 11 | human | 79083 | MLPH | melanophilin | NR_104019.2 | 45.7% | 1_211del;1751_1939del;2201_3933del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1866
- ORF length:
- 1800
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagaaactg gatctttcca agctcactga tgaagaggcc cagcatgtct 121 tggaagttgt tcaacgagat tttgacctcc gaaggaaaga agaggaacgg ctagaggcgt 181 tgaagggcaa gattaagaag gaaagctcca agagggagct gctttccgac actgcccatc 241 tgaacgagac ccactgcgcc cgctgcctgc agccctacca gctgcttgtg aatagcaaaa 301 ggcagtgcct ggaatgtggc ctcttcacct gcaaaagctg tggccgcgtc cacccggagg 361 agcagggctg gatctgtgac ccctgccatc tggccagagt cgtgaagatc ggctcactgg 421 agtggtacta tgagcatgtg aaagcccgct tcaagaggtt cggaagtgcc aaggtcatcc 481 ggtccctcca cgggcggctg cagggtggag ctgggcctga actgatatct gaagagagaa 541 gtggagacag cgaccagaca gatgaggatg gagaacctgg ctcagaggcc caggcccagg 601 cccagccctt tggcagcaaa aaaaagcgcc tcctctccgt ccacgacttc gacttcgagg 661 gagactcaga tgactccact cagcctcaag gtcactccct gcacctgtcc tcagtccctg 721 aggccaggga cagcccacag tccctcacag atgagtcctg ctcagagaag gcagcccctc 781 acaaggctga gggcctggag gaggctgata ctggggcctc tgggtgccac tcccatccgg 841 aagagcagcc gaccagcatc tcaccttcca gacacggcgc cctggctgag ctctgcccgc 901 ctggaggctc ccacaggatg gccctgggga ctgctgctgc actcgggtcg aatgtcatca 961 ggaatgagca gctgcccctg cagtacttgg ccgatgtgga cacctctgat gaggaaagca 1021 tccgggctca cgtgatggcc tcccaccatt ccaagcggag aggccgggcg tcttctgaga 1081 gtcagatctt tgagctgaat aagcatattt cagctgtgga atgcctgctg acctacctgg 1141 agaacacagt tgtgcctccc ttggccaagg gtctaggtgc tggagtgcgc acggaggccg 1201 atgtagagga ggaggccctg aggaggaagc tggaggagct gaccagcaac gtcagtgacc 1261 aggagacctc gtccgaggag gaggaagcca aggacgaaaa ggcagagccc aacagggaca 1321 aatcagttgg gcctctcccc caggcggacc cggaggtggg cacggctgcc catcaaacca 1381 acagacagga aaaaagcccc caggaccctg gggaccccgt ccagtacaac aggaccacag 1441 atgaggagct gtcagagctg gaggacagag tggcagtgac ggccTCAGAA GTCCAGCAGG 1501 CAGAGAGCGA GGTTTCAGAC ATTGAATCCA GGATTGCAGC CCTGAGGGCC GCAGGGCTCA 1561 CGGTGAAGCC CTCGGGAAAG CCCCGGAGGA AGTCAAACCT CCCGATATTT CTCCCTCGAG 1621 TGGCTGGGAA ACTTGGCAAG AGACCAGAGG ACCCAAATGC AGACCCTTCA AGTGAGGCCA 1681 AGGCAATGGC TGTGCCCTAT CTTCTGAGAA GAAAGTTCAG TAATTCCCTG AAAAGTCAAG 1741 GTAAAGATGA TGATTCTTTT GATCGGAAAT CAGTGTACCG AGGCTCGCTG ACACAGAGAA 1801 ACCCCAACGC GAGGAAAGGA ATGGCCAGCC ACACCTTCGC GAAACCTGTG GTGGCCCACC 1861 AGTCCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1921 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1981 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTACAGCT ACGCAATGAG CTCCCACGCG 2041 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt