Construct: ORF TRCN0000479846
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008591.1_s317c1
- Derived from:
- ccsbBroadEn_13487
- DNA Barcode:
- GAATGACCACGAACGTCGTGGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMIE (259236)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479846
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259236 | TMIE | transmembrane inner ear | NM_147196.3 | 99.3% | 99.3% | 367_369delAAG |
| 2 | human | 259236 | TMIE | transmembrane inner ear | NM_001370524.1 | 65.3% | 65.3% | 0_1ins159;208_210delAAG |
| 3 | human | 259236 | TMIE | transmembrane inner ear | NM_001370525.1 | 65.3% | 65.3% | 0_1ins159;208_210delAAG |
| 4 | mouse | 20776 | Tmie | transmembrane inner ear | NM_146260.2 | 87.8% | 91.6% | (many diffs) |
| 5 | mouse | 20776 | Tmie | transmembrane inner ear | XM_006512007.3 | 87.8% | 91.6% | (many diffs) |
| 6 | mouse | 20776 | Tmie | transmembrane inner ear | XM_006512008.3 | 87.8% | 91.6% | (many diffs) |
| 7 | mouse | 20776 | Tmie | transmembrane inner ear | XM_006512009.3 | 87.8% | 91.6% | (many diffs) |
| 8 | mouse | 20776 | Tmie | transmembrane inner ear | XM_006512010.3 | 87.8% | 91.6% | (many diffs) |
| 9 | mouse | 20776 | Tmie | transmembrane inner ear | XM_006512013.3 | 87.8% | 91.6% | (many diffs) |
| 10 | mouse | 20776 | Tmie | transmembrane inner ear | XM_011242944.2 | 87.8% | 91.6% | (many diffs) |
| 11 | mouse | 20776 | Tmie | transmembrane inner ear | XM_011242945.2 | 87.8% | 91.6% | (many diffs) |
| 12 | mouse | 20776 | Tmie | transmembrane inner ear | XM_006512014.3 | 59.7% | 63.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 534
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggggtgg ccgggcgcgg gtcccctctg cgtgctgggc ggcgccgcac 121 tcggggtgtg cctcgcgggg gttgccgggc agctggtgga gcccagcacg gccccaccca 181 agcccaagcc gccTCCGCTG ACCAAGGAGA CAGTGGTGTT CTGGGACATG CGCCTGTGGC 241 ACGTGGTGGG CATCTTTTCG CTCTTCGTGT TGTCCATCAT CATCACGCTG TGCTGTGTCT 301 TCAACTGTCG TGTGCCACGG ACCCGGAAGG AGATCGAAGC CCGGTACCTG CAGCGAAAGG 361 CAGCCAAGAT GTACACAGAC AAGCTGGAGA CTGTGCCACC CCTCAATGAG CTCACAGAAG 421 TCCCAGGAGA GGATAAGAAG AAGAAGAAGA AGAAGAAGGA CAGTGTGGAC ACAGTGGCCA 481 TCAAAGTAGA GGAGGATGAG AAGAATGAGG CCAAGAAGAA GAAAGGAGAG AAATTGCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAG AATGACCACG AACGTCGTGG GAAACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att