Construct: ORF TRCN0000479853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002978.1_s317c1
- Derived from:
- ccsbBroadEn_00473
- DNA Barcode:
- CGTACCAGTCTACACCCGTCGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR183 (1880)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1880 | GPR183 | G protein-coupled receptor 183 | NM_004951.5 | 100% | 100% | |
| 2 | human | 1880 | GPR183 | G protein-coupled receptor 183 | XM_017020405.2 | 100% | 100% | |
| 3 | mouse | 321019 | Gpr183 | G protein-coupled receptor 183 | NM_183031.2 | 81.1% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tatacaaatg gcaaacaatt ttactccgcc ctctgcaact cctcagggaa 121 atgactgtga cctctatgca catcacagca cggccaggat agtaatgcct ctgcattaca 181 gcctcgtctt catcattggg ctcgtgggaa acttactagc cttggtcgtc attgttcaaa 241 acaggaaaaa aatcaactct accaccctct attcaacaaa tttggtgatt tctgatatac 301 tttttaccac cgctttgcct acacgaatag cctactatgc aatgggcttt gactggagaa 361 tcggagatgc cttgtgtagg ataactgcgc tagtgtttta catcaacaca tatgcaggtg 421 tgaactttat gacctgcctg agtattgacc gcttcattgc tgtggtgcac cctctacgct 481 acaacaagat aaaaaggatt gaacatgcaa aaggcgtgtg catatttgtc tggattctag 541 tatttgctca gacactccca ctcctcatca accctatgtc aaagcaggag gctgaaagga 601 ttacatgcat ggagtatcca aactttgaag aaactaaatc tcttccctgg attctgcttg 661 gggcatgttt cataggatat gtacttccac ttataatcat tctcatctgc tattctcaga 721 tctgctgcaa actcttcaga actgccaaaC AAAACCCACT CACTGAGAAA TCTGGTGTAA 781 ACAAAAAGGC TCTCAACACA ATTATTCTTA TTATTGTTGT GTTTGTTCTC TGTTTCACAC 841 CTTACCATGT TGCAATTATT CAACATATGA TTAAGAAGCT TCGTTTCTCT AATTTCCTGG 901 AATGTAGCCA AAGACATTCG TTCCAGATTT CTCTGCACTT TACAGTATGC CTGATGAACT 961 TCAATTGCTG CATGGACCCT TTTATCTACT TCTTTGCATG TAAAGGGTAT AAGAGAAAGG 1021 TTATGAGGAT GCTGAAACGG CAAGTCAGTG TATCGATTTC TAGTGCTGTG AAGTCAGCCC 1081 CTGAAGAAAA TTCACGTGAA ATGACAGAAA CGCAGATGAT GATACATTCC AAGTCTTCAA 1141 ATGGAAAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGTACC AGTCTACACC CGTCGTGGAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt