Construct: ORF TRCN0000479853

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002978.1_s317c1
Derived from:
ccsbBroadEn_00473
DNA Barcode:
CGTACCAGTCTACACCCGTCGTGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GPR183 (1880)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479853
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1880 GPR183 G protein-coupled receptor 183 NM_004951.5 100% 100%
2 human 1880 GPR183 G protein-coupled receptor 183 XM_017020405.2 100% 100%
3 mouse 321019 Gpr183 G protein-coupled receptor 183 NM_183031.2 81.1% 86.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1149
ORF length:
1083
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tatacaaatg gcaaacaatt ttactccgcc ctctgcaact cctcagggaa
121atgactgtga cctctatgca catcacagca cggccaggat agtaatgcct ctgcattaca
181gcctcgtctt catcattggg ctcgtgggaa acttactagc cttggtcgtc attgttcaaa
241acaggaaaaa aatcaactct accaccctct attcaacaaa tttggtgatt tctgatatac
301tttttaccac cgctttgcct acacgaatag cctactatgc aatgggcttt gactggagaa
361tcggagatgc cttgtgtagg ataactgcgc tagtgtttta catcaacaca tatgcaggtg
421tgaactttat gacctgcctg agtattgacc gcttcattgc tgtggtgcac cctctacgct
481acaacaagat aaaaaggatt gaacatgcaa aaggcgtgtg catatttgtc tggattctag
541tatttgctca gacactccca ctcctcatca accctatgtc aaagcaggag gctgaaagga
601ttacatgcat ggagtatcca aactttgaag aaactaaatc tcttccctgg attctgcttg
661gggcatgttt cataggatat gtacttccac ttataatcat tctcatctgc tattctcaga
721tctgctgcaa actcttcaga actgccaaaC AAAACCCACT CACTGAGAAA TCTGGTGTAA
781ACAAAAAGGC TCTCAACACA ATTATTCTTA TTATTGTTGT GTTTGTTCTC TGTTTCACAC
841CTTACCATGT TGCAATTATT CAACATATGA TTAAGAAGCT TCGTTTCTCT AATTTCCTGG
901AATGTAGCCA AAGACATTCG TTCCAGATTT CTCTGCACTT TACAGTATGC CTGATGAACT
961TCAATTGCTG CATGGACCCT TTTATCTACT TCTTTGCATG TAAAGGGTAT AAGAGAAAGG
1021TTATGAGGAT GCTGAAACGG CAAGTCAGTG TATCGATTTC TAGTGCTGTG AAGTCAGCCC
1081CTGAAGAAAA TTCACGTGAA ATGACAGAAA CGCAGATGAT GATACATTCC AAGTCTTCAA
1141ATGGAAAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1201CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1261TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGTACC AGTCTACACC CGTCGTGGAC
1321GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)