Construct: ORF TRCN0000479860

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017494.1_s317c1
Derived from:
ccsbBroadEn_03699
DNA Barcode:
AAACGTTTACTGGGAGATGGATTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCDHGB5 (56101)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479860
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56101 PCDHGB5 protocadherin gamma subfami... NM_032099.1 100% 100%
2 human 56101 PCDHGB5 protocadherin gamma subfami... NM_018925.2 86.1% 86% (many diffs)
3 human 8641 PCDHGB4 protocadherin gamma subfami... NM_032098.1 82.6% 81.8% (many diffs)
4 human 8641 PCDHGB4 protocadherin gamma subfami... NM_003736.3 72.7% 71.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2523
ORF length:
2454
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggggagcggc gccggggagc tgggccgggc tgagaggctg ccagtgctct
121ttctcttcct gctgtctttg ttctgcccgg cgctctgtga gcagatccgc tacaggattc
181ccgaggaaat gcccaagggc tccgtagtgg ggaacctcgc cacggacctg gggttcagcg
241tccaggagtt accgactcga aaactgcgcg tcagttcgga gaagccttac ttcaccgtga
301gcgcagagag cggggagttg cttgtgagca gcaggctaga cagggaggag atatgcggga
361agaagccagc ttgtgctctg gaatttgagg ctgttgctga aaatccactg aacttttatc
421acgtgaatgt ggagatcgag gacattaatg accacacgcc aaaattcacg caaaattcct
481ttgagctgca aataagtgag tctgcacagc ctggcacaag atttatacta gaagtagcag
541aagatgcaga tattggctta aactctctgc agaagtataa actctctctt aacccaagtt
601tctcattaat aattaaggag aaacaggatg gtagtaaata cccggaactg gcactggaga
661aaaccttaga ccgggaacaa cagagttacc atcgtttagt cctgactgcc ttggacggtg
721gacatccacc cctaagcggc accactgagc tccggatcca ggtaaccgac gccaatgata
781atcccccggt attcaaccga gacgtgtaca gagtcagcct tcgggaaaac gtgccaccag
841gcaccactgt gttgcaagtg tcagccactg accaagacga gggcatcaac tcagaaatta
901cttattcctt ctacagaacc gggcaaatct ttagtctgaa ttcaaagagc ggagaaatta
961ccactcaaaa gaaactggat tttgaagaga ccaaggaata ttcaatggtt gtagaaggga
1021gggatggtgg tggactggtt gcacaatgta cagttgaaat taatattcaa gatgaaaatg
1081acaatagccc agaagttaca ttccattctc tacttgaaat gattctggaa aacgcggtgc
1141ctggaacact aattgctttg atcaaaatac atgaccaaga ttctggggaa aatggggagg
1201ttaattgtca attacaaggc gaagtccctt ttaagattat ctcttcatcc aaaaattcgt
1261ataagttggt aacagatgga accctagacc gagagcaaac cccggagtac aatgtcacca
1321tcacagccac agacaggggc aagccgcccc tctcctccag cataagcgtc atcctacata
1381tcagagacgt caacgataac gctccggttt tccaccaggc gtcctactta gtcagtgtac
1441ccgaaaacaa ccctcctggg gcctccatcg cgcaagtctg cgcctcggac ctggacttgg
1501ggttgaacgg ccaagtctcc tactctatca tggccagcga cctagagcct ctggcactgg
1561cctcttacgt gtccatgagc gcgcaaagtg gggtggtgtt cgcgcagcgc gcctttgact
1621acgagcagct gcgcaccttc gaactcacac tacaggcccg cgaccagggc tcgcctgcgc
1681tcagcgcaaa cgtgagcctg cgcgtgttgg tgggcgaccg aaacgacaac gcaccgcggg
1741tgctgtaccc cgcgctgggt cccgacggct ctgcgctctt cgatatggtg ccgcgcgctg
1801cagagcccgg ctacctggtg accaaggtag tggccgtgga cgcagactca ggacacaacg
1861cctggctgtc ctaccacgtg ctgcaggcta gcgagcccgg gctcttcagc ctggggctgc
1921gcacaggaga ggtgcgcaca gcgcgtgcct tgggcgacag ggacgcggcc cgccagcgcc
1981tgctggttgc tgtgcgtgat ggtggacagc cgccactctc cgccaccgcc acgctgcact
2041tggtctttgc tgacagcttg caggaggtgc tgccggatat cactgaccgc cctgtaccct
2101ctgaccccca ggctgagctg cagttttacc tagtggtggc cttggccttg atctcagtgc
2161tcttcctcct ggccgtgatt ctggccgttg ccttgcgccT GCGACGCTCC TCCAGCCCTG
2221CCGCCTGGAG CTGCTTCCAA CCTGGTCTCT GTGTCAAGTC TGGACCTGTG GTTCCCCCCA
2281ACTACAGTCA GGGGACTTTG CCTTATTCCT ACAACCTATG TGTTGCACAT ACAGGAAAGA
2341CGGAGTTTAA TTTCCTAAAA TGTAGTGAGC AATTGAGTTC AGGACAAGAC ATACTTTGTG
2401GTGATTCATC TGGGGCCTTA TTTCCACTTT GTAATTCCAG CGAGTCGACT TCCCATCCTG
2461AGTTGGTGAG TTTCATTTAT GTCTATTCTT TTTCATTACC CACCCAATTT TCTGTATTTA
2521CATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2581TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2641GGCTTTATAT ATCTTGTGGA AAGGACGAAA ACGTTTACTG GGAGATGGAT TCACGCGTTA
2701AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

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