Construct: ORF TRCN0000479860
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017494.1_s317c1
- Derived from:
- ccsbBroadEn_03699
- DNA Barcode:
- AAACGTTTACTGGGAGATGGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGB5 (56101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479860
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56101 | PCDHGB5 | protocadherin gamma subfami... | NM_032099.1 | 100% | 100% | |
| 2 | human | 56101 | PCDHGB5 | protocadherin gamma subfami... | NM_018925.2 | 86.1% | 86% | (many diffs) |
| 3 | human | 8641 | PCDHGB4 | protocadherin gamma subfami... | NM_032098.1 | 82.6% | 81.8% | (many diffs) |
| 4 | human | 8641 | PCDHGB4 | protocadherin gamma subfami... | NM_003736.3 | 72.7% | 71.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2523
- ORF length:
- 2454
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggagcggc gccggggagc tgggccgggc tgagaggctg ccagtgctct 121 ttctcttcct gctgtctttg ttctgcccgg cgctctgtga gcagatccgc tacaggattc 181 ccgaggaaat gcccaagggc tccgtagtgg ggaacctcgc cacggacctg gggttcagcg 241 tccaggagtt accgactcga aaactgcgcg tcagttcgga gaagccttac ttcaccgtga 301 gcgcagagag cggggagttg cttgtgagca gcaggctaga cagggaggag atatgcggga 361 agaagccagc ttgtgctctg gaatttgagg ctgttgctga aaatccactg aacttttatc 421 acgtgaatgt ggagatcgag gacattaatg accacacgcc aaaattcacg caaaattcct 481 ttgagctgca aataagtgag tctgcacagc ctggcacaag atttatacta gaagtagcag 541 aagatgcaga tattggctta aactctctgc agaagtataa actctctctt aacccaagtt 601 tctcattaat aattaaggag aaacaggatg gtagtaaata cccggaactg gcactggaga 661 aaaccttaga ccgggaacaa cagagttacc atcgtttagt cctgactgcc ttggacggtg 721 gacatccacc cctaagcggc accactgagc tccggatcca ggtaaccgac gccaatgata 781 atcccccggt attcaaccga gacgtgtaca gagtcagcct tcgggaaaac gtgccaccag 841 gcaccactgt gttgcaagtg tcagccactg accaagacga gggcatcaac tcagaaatta 901 cttattcctt ctacagaacc gggcaaatct ttagtctgaa ttcaaagagc ggagaaatta 961 ccactcaaaa gaaactggat tttgaagaga ccaaggaata ttcaatggtt gtagaaggga 1021 gggatggtgg tggactggtt gcacaatgta cagttgaaat taatattcaa gatgaaaatg 1081 acaatagccc agaagttaca ttccattctc tacttgaaat gattctggaa aacgcggtgc 1141 ctggaacact aattgctttg atcaaaatac atgaccaaga ttctggggaa aatggggagg 1201 ttaattgtca attacaaggc gaagtccctt ttaagattat ctcttcatcc aaaaattcgt 1261 ataagttggt aacagatgga accctagacc gagagcaaac cccggagtac aatgtcacca 1321 tcacagccac agacaggggc aagccgcccc tctcctccag cataagcgtc atcctacata 1381 tcagagacgt caacgataac gctccggttt tccaccaggc gtcctactta gtcagtgtac 1441 ccgaaaacaa ccctcctggg gcctccatcg cgcaagtctg cgcctcggac ctggacttgg 1501 ggttgaacgg ccaagtctcc tactctatca tggccagcga cctagagcct ctggcactgg 1561 cctcttacgt gtccatgagc gcgcaaagtg gggtggtgtt cgcgcagcgc gcctttgact 1621 acgagcagct gcgcaccttc gaactcacac tacaggcccg cgaccagggc tcgcctgcgc 1681 tcagcgcaaa cgtgagcctg cgcgtgttgg tgggcgaccg aaacgacaac gcaccgcggg 1741 tgctgtaccc cgcgctgggt cccgacggct ctgcgctctt cgatatggtg ccgcgcgctg 1801 cagagcccgg ctacctggtg accaaggtag tggccgtgga cgcagactca ggacacaacg 1861 cctggctgtc ctaccacgtg ctgcaggcta gcgagcccgg gctcttcagc ctggggctgc 1921 gcacaggaga ggtgcgcaca gcgcgtgcct tgggcgacag ggacgcggcc cgccagcgcc 1981 tgctggttgc tgtgcgtgat ggtggacagc cgccactctc cgccaccgcc acgctgcact 2041 tggtctttgc tgacagcttg caggaggtgc tgccggatat cactgaccgc cctgtaccct 2101 ctgaccccca ggctgagctg cagttttacc tagtggtggc cttggccttg atctcagtgc 2161 tcttcctcct ggccgtgatt ctggccgttg ccttgcgccT GCGACGCTCC TCCAGCCCTG 2221 CCGCCTGGAG CTGCTTCCAA CCTGGTCTCT GTGTCAAGTC TGGACCTGTG GTTCCCCCCA 2281 ACTACAGTCA GGGGACTTTG CCTTATTCCT ACAACCTATG TGTTGCACAT ACAGGAAAGA 2341 CGGAGTTTAA TTTCCTAAAA TGTAGTGAGC AATTGAGTTC AGGACAAGAC ATACTTTGTG 2401 GTGATTCATC TGGGGCCTTA TTTCCACTTT GTAATTCCAG CGAGTCGACT TCCCATCCTG 2461 AGTTGGTGAG TTTCATTTAT GTCTATTCTT TTTCATTACC CACCCAATTT TCTGTATTTA 2521 CATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2581 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2641 GGCTTTATAT ATCTTGTGGA AAGGACGAAA ACGTTTACTG GGAGATGGAT TCACGCGTTA 2701 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt