Construct: ORF TRCN0000479863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016335.1_s317c1
- Derived from:
- ccsbBroadEn_11152
- DNA Barcode:
- AGACGTTTCATAGCCTACAGAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SP100 (6672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6672 | SP100 | SP100 nuclear antigen | NM_001206702.1 | 99.9% | 100% | 1257G>A |
| 2 | human | 6672 | SP100 | SP100 nuclear antigen | NM_001206703.1 | 94% | 94.1% | 30_31ins75;1182G>A;1206_1207insGGTGAGAAG |
| 3 | human | 6672 | SP100 | SP100 nuclear antigen | NM_001206704.1 | 92.5% | 92.5% | 0_1ins105;2T>G;1152G>A |
| 4 | human | 6672 | SP100 | SP100 nuclear antigen | NM_001206701.2 | 69.5% | 69.3% | (many diffs) |
| 5 | human | 6672 | SP100 | SP100 nuclear antigen | NM_003113.4 | 54.4% | 54.2% | (many diffs) |
| 6 | human | 6672 | SP100 | SP100 nuclear antigen | NM_001080391.2 | 54% | 53.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggtgggggc ggcgacctga gcaccaggag gctgaatgaa tgtatttcac 121 cagtagcaaa tgagatgaac catcttcctg cacacagcca cgatttgcaa aggatgttca 181 cggaagacca gggtgtagat gacaggctgc tctatgacat tgtattcaag cacttcaaaa 241 gaaataaggt ggagatttca aatgcaataa aaaagacatt tccattcctc gagggcctcc 301 gtgatcgtga tctcatcaca aataaaatgt ttgaagattc tcaagattct tgtagaaacc 361 tggtccctgt acagagagtg gtgtacaatg ttcttagtga actggagaag acatttaacc 421 tgccagttct ggaagcactg ttcagcgatg tcaacatgca ggaatacccc gatttaattc 481 acatttataa aggctttgaa aatgtaatcc atgacaaatt gcctctccaa gaaagtgaag 541 aagaagagag ggaggagagg tctggcctcc aactaagtct tgaacaagga actggtgaaa 601 actcttttcg aagcctgact tggccacctt cgggttcccc atctcatgct ggtacaaccc 661 cacctgaaaa tggactctca gagcacccct gtgaaacaga acagataaat gcaaagagaa 721 aagatacaac cagtgacaaa gatgattcgc taggaagcca acaaacaaat gaacaatgtg 781 ctcaaaaggc tgagccaaca gagtcctgcg aacaaattgc tgtccaagtg aataatgggg 841 atgctggaag ggagatgccc tgcccgttgc cctgtgatga agaaagccca gaggcagagc 901 tacacaacca tggaatccaa attaattcct gttctgtgcg actggtggat ataaaaaagg 961 aaaagccatt ttctaattca aaagttgagt gccaagccca agcaagaact catcataacc 1021 aggcatctga cataatagtc atcagcagtg aggactctga aggatccact gacgttgatg 1081 agcccttaga agtcttcatc TCAGCACCGA GAAGTGAGCC TGTGATCAAT AATGACAACC 1141 CTTTAGAATC AAATGATGAA AAGGAGGGCC AAGAAGCCAC TTGCTCACGA CCCCAGATTG 1201 TACCAGAGCC CATGGATTTC AGAAAATTAT CTACATTCAG AGAAAGTTTT AAGAAAAGAG 1261 TGATAGGACA AGACCACGAC TTTTCAGAAT CCAGTGAGGA GGAGGCGCCC GCAGAAGCCT 1321 CAAGCGGGGC ACTGAGAAGC AAGCATGGTG AGAAGGCTCC TATGACTTCT AGAAGTACAT 1381 CTACTTGGAG AATACCCAGC AGGAAGAGAC GTTTCAGCAG TAGTGACTTT TCAGACCTGA 1441 GTAATGGAGA AGAGCTTCAG GAAACCTGCA GCTCATCCCT AAGAAGAGGG TCAGGTAAAG 1501 AAGATTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGACGTTTC ATAGCCTACA GAGGCACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt