Construct: ORF TRCN0000479880
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002471.1_s317c1
- Derived from:
- ccsbBroadEn_11677
- DNA Barcode:
- ACATTTGGACCATGCGTCAATGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FSTL4 (23105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479880
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23105 | FSTL4 | follistatin like 4 | NM_015082.2 | 71.7% | 71.3% | (many diffs) |
| 2 | human | 23105 | FSTL4 | follistatin like 4 | XM_011543283.1 | 71.7% | 71.3% | (many diffs) |
| 3 | human | 23105 | FSTL4 | follistatin like 4 | XM_011543284.2 | 70.6% | 70.1% | (many diffs) |
| 4 | human | 23105 | FSTL4 | follistatin like 4 | XM_011543286.3 | 54.9% | 54.6% | (many diffs) |
| 5 | human | 23105 | FSTL4 | follistatin like 4 | XM_017009251.1 | 34.3% | 34% | (many diffs) |
| 6 | human | 23105 | FSTL4 | follistatin like 4 | XM_011543287.2 | 33.2% | 32.8% | (many diffs) |
| 7 | mouse | 320027 | Fstl4 | follistatin-like 4 | NM_177059.3 | 60.6% | 60.8% | (many diffs) |
| 8 | mouse | 320027 | Fstl4 | follistatin-like 4 | XM_006533539.3 | 23.6% | 24.3% | (many diffs) |
| 9 | mouse | 320027 | Fstl4 | follistatin-like 4 | XR_388477.3 | 22% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1881
- ORF length:
- 1815
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa accaggaggc ttttggctgc atctcacact gctcggagcc tccctgccgg 121 ctgcgctggg atggatggac ccaggaacca gcagaggccc ggatgtgggt gtgggggagt 181 cacaggcaga ggagcccaga agctttgaag tcacaagaag agaagggctt tccagccaca 241 acgagctgct ggcctcctgc gggaagaagt tctgcagccg agggagccgg tgcgtgctca 301 gcaggaagac aggggagccc gaatgccagt gcctggaggc atgcaggccc agctacgtgc 361 ctgtgtgcgg ctctgatggg aggttttatg aaaaccactg taagctccac cgtgctgctt 421 gcctcctggg aaagaggatc accgtcatcc acagcaagga ctgtttcctc aaaggtgaca 481 cgtgcaccat ggccggctac gcccgcttga agaatgtcct tctggcactc cagacccgtc 541 tgcagccact ccaagaagga gacagcagac aagaccctgc ctcccagaag cgcctcctgg 601 tggaatctct gttcagggac ttagatgcag atggcaatgg ccacctcagc agctccgaac 661 tggctcagca tgtgctgaag aagcaggacc tggatgaaga cttacttggt tgctcaccag 721 gtgacctcct ccgatttgac gattacaaca gtgacagctc cctgaccctc cgcgagttct 781 acattgcctt ccaagtggtt cagctcagcc tcgcccccga ggacagggtc agtgtgacca 841 cagtgaccgt ggggctgagc acagtgctga cctgcgccgt ccatggagac ctgaggccac 901 caatcatctg gaagcgcaac gggctcaccc tgaacttcct ggacttggaa gacatcaatg 961 actttggaga ggatgattcc ctgtacatca ccaaggtgac caccatccac atgggcaatt 1021 acacctgcca tgcttccggc cacgagcagc tgttccagac ccacgtcctg caggtgaatg 1081 tgccgccagt catccgtgtc tatccagaga gccaggcaca ggagcctgga gtggcagcca 1141 gcctaagatg ccatgctgag ggcattccca tgcccagaat cacttggctg aaaaacggcg 1201 tggatgtctc aactcagatg tccaaacagc tctccctttt agccaatggg agcgaactcc 1261 acatcagcag tgttcggtat gaagacacag gggcatacac ctgcattgcc aaaaatgaag 1321 tgggtgtgga tgaagatatc tcctcgctct tcattgaaga ctcagctaga aagacccttg 1381 caaacatcct gtggcgagag gaaggcctca gcgtgggaaa catgttctat gtcttctccg 1441 acgacggtat catcgtcatc catcctgtgg actgtgagat ccagaggcac ctcaaaccca 1501 cggaaaagat tttcatgagc tatgaagaaa tctgtccTCA AAGAGAAAAA AATGCAACCC 1561 AGCCCTGCCA GTGGGTATCT GCAGTCAATG TCCGGAACCG GTACATCTAT GTGGCCCAGC 1621 CAGCACTGAG CAGAGTCCTT GTGGTCGACA TCCAAGCCCA GAAAGTCCTA CAGTCCATAG 1681 GTGTGGACCC TCTGCCGGCT AAGCTGTCCT ATGACAAGTC ACATGACCAA GTGTGGGTCC 1741 TGAGCTGGGG GGACGTGCAC AAGTCCCGAC CAAGTCTCCA GGTGATCACA GAAGCCAGCA 1801 CCGGCCAGAG CCAGCACCTC ATCCGCACAC CCTTTGCAGG AGTGGATGAT TTCTTCATTC 1861 CCCCAATAAA CCTTGAACAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1921 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1981 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAT TTGGACCATG 2041 CGTCAATGCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt