Construct: ORF TRCN0000479898
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016080.1_s317c1
- Derived from:
- ccsbBroadEn_00025
- DNA Barcode:
- TTAACCAATCCCAATATTTGCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCYAP1 (116)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479898
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116 | ADCYAP1 | adenylate cyclase activatin... | NM_001099733.2 | 100% | 100% | |
| 2 | human | 116 | ADCYAP1 | adenylate cyclase activatin... | NM_001117.5 | 100% | 100% | |
| 3 | human | 116 | ADCYAP1 | adenylate cyclase activatin... | XM_005258081.4 | 55.8% | 55.8% | 1_417del |
| 4 | mouse | 11516 | Adcyap1 | adenylate cyclase activatin... | NM_001315503.1 | 85% | 82.3% | (many diffs) |
| 5 | mouse | 11516 | Adcyap1 | adenylate cyclase activatin... | NM_009625.3 | 85% | 82.3% | (many diffs) |
| 6 | mouse | 11516 | Adcyap1 | adenylate cyclase activatin... | XM_006523498.3 | 78.1% | 74.3% | (many diffs) |
| 7 | mouse | 11516 | Adcyap1 | adenylate cyclase activatin... | XM_006523499.3 | 64.3% | 58.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 597
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccatgtgt agcggagcga ggctggccct gctggtctat gggataatca 121 tgcacagcag cgtctacagc tcacctgccg ccgccggact ccggttcccc gggatcaggc 181 cagaggaaga ggcgtacggc gaggacggaa acccgctgcc agacttcgat ggctcggagc 241 cgccgggcgc agggagcccc gccTCCGCGC CGCGCGCCGC CGCCGCCTGG TACCGCCCGG 301 CCGGGAGAAG AGATGTCGCC CACGGGATCC TTAACGAGGC CTACCGCAAA GTGCTGGACC 361 AGCTGTCCGC CGGGAAGCAC CTGCAGTCGC TCGTGGCCCG GGGCGTGGGT GGGAGCCTCG 421 GCGGCGGCGC GGGGGACGAC GCGGAGCCGC TCTCCAAGCG CCACTCGGAC GGGATCTTCA 481 CGGACAGCTA CAGCCGCTAC CGGAAACAAA TGGCTGTCAA GAAATACTTG GCGGCCGTCC 541 TAGGGAAGAG GTATAAACAA AGGGTTAAAA ACAAAGGACG CCGAATAGCT TATTTGTTGC 601 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 661 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 721 ATATATCTTG TGGAAAGGAC GATTAACCAA TCCCAATATT TGCCAAACGC GTTAAGTCga 781 caatcaacct ctggattaca aaatttgtga aagatt