Construct: ORF TRCN0000479905
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006348.1_s317c1
- Derived from:
- ccsbBroadEn_13621
- DNA Barcode:
- CCCTCTTGGAAGACATGCCGCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CFAP100 (348807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479905
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 348807 | CFAP100 | cilia and flagella associat... | NM_182628.3 | 99.8% | 99.8% | 416_417insAGC |
| 2 | human | 348807 | CFAP100 | cilia and flagella associat... | XM_006713623.2 | 99.8% | 99.8% | 416_417insAGC |
| 3 | human | 348807 | CFAP100 | cilia and flagella associat... | XM_017006322.1 | 73.8% | 49.1% | 416_417insAGC;953_954insAGAGGGTC;1356_1357ins469 |
| 4 | human | 348807 | CFAP100 | cilia and flagella associat... | XM_017006323.1 | 70.9% | 52.3% | (many diffs) |
| 5 | human | 348807 | CFAP100 | cilia and flagella associat... | XM_017006321.1 | 65.5% | 59.8% | (many diffs) |
| 6 | human | 348807 | CFAP100 | cilia and flagella associat... | XR_001740141.2 | 63.3% | (many diffs) | |
| 7 | human | 348807 | CFAP100 | cilia and flagella associat... | XM_017006325.1 | 61.2% | 59.2% | (many diffs) |
| 8 | human | 348807 | CFAP100 | cilia and flagella associat... | XM_017006326.1 | 59.4% | 59.1% | (many diffs) |
| 9 | human | 348807 | CFAP100 | cilia and flagella associat... | XM_017006327.1 | 58.9% | 51.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1905
- ORF length:
- 1836
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgagata ccgtccacta tagtctccaa gaacatgacc aatgacaaga 121 acagcctgga atccatgaac atcagctctt cttcaagcac tgaagagaac ccaaagaaac 181 aggcaagaaa aaacgaagaa catggtcctg acccttcagc gaaccctttc cacttatctg 241 gggatgtgga tttcttcttg ctcagagatc aggagcggaa taaggctctc tccgaacggc 301 agcagcagaa gacgatgcgg gtgcaccaga agatgaccta ctcctcgaaa gtgtcggcta 361 agcacaccag cctgcggcgg cagctgcagc tggaggacaa gcaggaggac ctggaggcgc 421 gcgccgaggc cgagcatcag cgcgccttcc gcgactacac gacctggaag ctcaccttga 481 ccaaagcaga aaagaatgtg gagcctgaga acatgagtgg ctacattaag cagaagcggc 541 aaatgttcct cctccagtat gccctggatg tcaagcggag agagatccag cggctggaga 601 cgctggcgac caaagaggag gccaggctgg agcgggccga gaaatccctg gagaaggacg 661 ccgccttgtt cgacgagttc gtcagggaga atgactgcag ctccgtgcag gccatgagag 721 cggctgagaa ggagaccaaa gccaagatag agaagatcct tgagatccgg gacctcacca 781 cccagattgt taatatcaaa agtgagatct ccagatttga agacactctg aagcattaca 841 aggtctataa ggatttccta tacaagctgt cgcccaagga gtggcttgaa gaacaggaaa 901 agaaacactc gtttctcaaa aaggccaagg aggtctccga ggcttccaaa gagagcagtg 961 ttaactccac accaggggac aaaggaccag ggatcaaggg caaggcgagc tccatgtggg 1021 ccaaagaggg tcagggtaca aagaagccct ggaggtttct gcagacgatg cggctggggc 1081 ggagcccgtc ttacctgagc agcccccagc aaggcagcca gcccagcgag tccagcggtg 1141 gcgactccag agggtcgaac tctcccatcc cccccacgca ggaggacacc gacagcgatg 1201 gggaggaacc acagctgtac ttcacggagc cccagcagct cctggatgtc ttccgagagc 1261 tggaggagca gaacctgtcg ctgatccaga acagccagga gacggagaag accctggagg 1321 agctgagcca caccctgaaa cacacccaga tccgcatgga cagggaggtc aaccagctga 1381 agcagtgggt caccacaatg atgatgtcca tcaccaagga ggaggacaca gcagctgagc 1441 tggagctcaa agcccgagtc ttccacttcg gcgagtacaa gggcgatcag caggataagc 1501 tgctagagag ccTGAACTGC AAGGTGCTGG ATGTGTACCG GCACTGCACC GGCACCCAGC 1561 AGGAGGCCAA CCTGGGCACC GTGCAGATGC TGACCATCAT TGAGCACCAG CTGGATGAGC 1621 TGCTAGAGAA CCTGGAGCAC GTGCCCCAGG TCAAGATCGA GCAGGCCGAG AGGGCAAAGG 1681 AGAAGGAGCG GCGCATCAGA CTTCGAGAAG AGAAGCTCCA GATGCAAAAG ATCCTACAGG 1741 AGGAGCATCT GCAGCGGGCC CGGGCGCGCG CCCAGGCTGA GATCAAGAAG AAGAGAGGCA 1801 GGACACTGGT ATGCCGCTCA CGACCCCCAG CCCACAGGAT CAAACAACAG TCTGAGCACA 1861 CACTGATGGA CAAGGAGGAG GAGGAGCTGC TATTTTTCTT TACTTTGCCA ACTTTCTTGT 1921 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1981 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2041 GAAAGGACGA CCCTCTTGGA AGACATGCCG CAGGACGCGT TAAGTCgaca atcaacctct 2101 ggattacaaa atttgtgaaa gatt