Construct: ORF TRCN0000479921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005550.3_s317c1
- Derived from:
- ccsbBroadEn_15053
- DNA Barcode:
- CTGCACCCACATCTAGGCCCCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NRBP1 (29959)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479921
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321357.1 | 100% | 100% | |
| 2 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_013392.4 | 100% | 100% | |
| 3 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321362.2 | 99.8% | 99.8% | 743_744insAGA |
| 4 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321358.2 | 98.5% | 98.5% | 660_683del |
| 5 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321359.2 | 98.5% | 98.5% | 660_683del |
| 6 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321361.2 | 98.3% | 98.3% | 660_683del;926_927insGGA |
| 7 | human | 29959 | NRBP1 | nuclear receptor binding pr... | NM_001321363.2 | 94% | 94% | 338_339ins96 |
| 8 | mouse | 192292 | Nrbp1 | nuclear receptor binding pr... | NM_147201.2 | 93.2% | 98.3% | (many diffs) |
| 9 | mouse | 192292 | Nrbp1 | nuclear receptor binding pr... | NM_001347326.1 | 91.8% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1671
- ORF length:
- 1605
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggagggggag tcccagacag tacttagcag tggctcagac ccaaaggtag 121 aatcctcatc ttcagctcct ggcctgacat cagtgtcacc tcctgtgacc tccacaacct 181 cagctgcttc cccagaggaa gaagaagaaa gtgaagatga gtctgagatt ttggaagagt 241 cgccctgtgg gcgctggcag aagaggcgag aagaggtgaa tcaacggaat gtaccaggta 301 ttgacagtgc atacctggcc atggatacag aggaaggtgt agaggttgtg tggaatgagg 361 tacagttctc tgaacgcaag aactacaagc tgcaggagga aaaggttcgt gctgtgtttg 421 ataatctgat tcaattggag catcttaaca ttgttaagtt tcacaaatat tgggctgaca 481 ttaaagagaa caaggccagg gtcattttta tcacagaata catgtcatct gggagtctga 541 agcaatttct gaagaagacc aaaaagaacc acaagacgat gaatgaaaag gcatggaagc 601 gttggtgcac acaaatcctc tctgccctaa gctacctgca ctcctgtgac ccccccatca 661 tccatgggaa cctgacctgt gacaccatct tcatccagca caacggactc atcaagattg 721 gctctgtggc tcctgacact atcaacaatc atgtgaagac ttgtcgagaa gagcagaaga 781 atctacactt ctttgcacca gagtatggag aagtcactaa tgtgacaaca gcagtggaca 841 tctactcctt tggcatgtgt gcactggaga tggcagtgct ggagattcag ggcaatggag 901 agtcctcata tgtgccacag gaagccatca gcagtgccat ccagcttcta gaagacccat 961 tacagaggga gttcattcaa aagtgcctgc agtctgagcc tgctcgcaga ccaacagcca 1021 gagaacttct gttccaccca gcattgtttg aagtgccctc gctcaaactc cttgcggccc 1081 actgcattgt gggacaccaa cacatgatcc cagagaacgc tctagaggag atcaccaaaa 1141 acatggatac tagtgccgta ctggctgaaa tccctgcagg accaggaaga gaaccagttc 1201 agactttgta ctctcagtca ccagctctgg aattagataa attccttgaa gatgtcagga 1261 atgggatcta tcctctgaca gcctttgggc tgccTCGGCC CCAGCAGCCA CAGCAGGAGG 1321 AGGTGACATC ACCTGTCGTG CCCCCCTCTG TCAAGACTCC GACACCTGAA CCAGCTGAGG 1381 TGGAGACTCG CAAGGTGGTG CTGATGCAGT GCAACATTGA GTCGGTGGAG GAGGGAGTCA 1441 AACACCACCT GACACTTCTG CTGAAGTTGG AGGACAAACT GAACCGGCAC CTGAGCTGTG 1501 ACCTGATGCC AAATGAGAAT ATCCCCGAGT TGGCGGCTGA GCTGGTGCAG CTGGGCTTCA 1561 TTAGTGAGGC TGACCAGAGC CGGTTGACTT CTCTGCTAGA AGAGACCTTG AACAAGTTCA 1621 ATTTTGCCAG GAACAGTACC CTCAACTCAG CCGCTGTCAC CGTCTCCTCT TACCCAACTT 1681 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1741 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1801 CTTGTGGAAA GGACGACTGC ACCCACATCT AGGCCCCACG ACGCGTTAAG TCgacaatca 1861 acctctggat tacaaaattt gtgaaagatt