Construct: ORF TRCN0000479935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011062.1_s317c1
- Derived from:
- ccsbBroadEn_11462
- DNA Barcode:
- CTAACCTAAAAATTACGGTACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHST4 (10164)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479935
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10164 | CHST4 | carbohydrate sulfotransfera... | NM_001166395.2 | 95.8% | 95.8% | 1_48del |
| 2 | human | 10164 | CHST4 | carbohydrate sulfotransfera... | NM_005769.2 | 95.8% | 95.8% | 1_48del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catcttggct ctattcttcc acatgtacag ccacaacatc agctccctgt 121 ctatgaaggc acagcccgag cgcatgcacg tgctggttct gtcttcctgg cgctctggct 181 cttcttttgt ggggcagctt tttgggcagc acccagatgt tttctacctg atggagcccg 241 cctggcacgt gtggatgacc ttcaagcaga gcaccgcctg gatgctgcac atggctgtgc 301 gggatctgat acgggccgtc ttcttgtgcg acatgagcgt ctttgatgcc tacatggaac 361 ctggtccccg gagacagtcc agcctctttc agtgggagaa cagccgggcc ctgtgttctg 421 cacctgcctg tgacatcatc ccacaagatg aaatcatccc ccgggctcac tgcaggctcc 481 tgtgcagtca acagcccttt gaggtggtgg agaaggcctg ccgctcctac agccacgtgg 541 tgctcaagga ggtgcgcttc ttcaacctgc agtccctcta cccgctgctg aaagacccct 601 ccctcaacct gcatatcgtg cacctggtcc gggacccccg ggccgtgttc cgttcccgag 661 aacgcacaaa gggagatctc atgattgaca gtcgcattgt gatggggcag catgagcaaa 721 aactcaagaa ggaggaccaa cccTACTATG TGATGCAGGT CATCTGCCAA AGCCAGCTGG 781 AGATCTACAA GACCATCCAG TCCTTGCCCA AGGCCCTGCA GGAACGCTAC CTGCTTGTGC 841 GCTATGAGGA CCTGGCTCGA GCCCCTGTGG CCCAGACTTC CCGAATGTAT GAATTCGTGG 901 GATTGGAATT CTTGCCCCAT CTTCAGACCT GGGTGCATAA CATCACCCGA GGCAAGGGCA 961 TGGGTGACCA CGCTTTCCAC ACAAATGCCA GGGATGCCCT TAATGTCTCC CAGGCTTGGC 1021 GCTGGTCTTT GCCCTATGAA AAGGTTTCTC GACTTCAGAA AGCCTGTGGC GATGCCATGA 1081 ATTTGCTGGG CTACCGCCAC GTCAGATCTG AACAAGAACA GAGAAACCTG TTGCTGGATC 1141 TTCTGTCTAC CTGGACTGTC CCTGAGCAAA TCCACTACCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 ACTAACCTAA AAATTACGGT ACTTTACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt