Transcript: Human NM_001166395.2

Homo sapiens carbohydrate sulfotransferase 4 (CHST4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
CHST4 (10164)
Length:
2159
CDS:
89..1249

Additional Resources:

NCBI RefSeq record:
NM_001166395.2
NBCI Gene record:
CHST4 (10164)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001166395.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000034836 GACCACGCTTTCCACACAAAT pLKO.1 1037 CDS 100% 13.200 18.480 N CHST4 n/a
2 TRCN0000430817 CAAAGGGAGATCTCATGATTG pLKO_005 738 CDS 100% 10.800 15.120 N CHST4 n/a
3 TRCN0000435874 TGTGCGACATGAGCGTCTTTG pLKO_005 396 CDS 100% 10.800 15.120 N CHST4 n/a
4 TRCN0000034837 GATGGCCATCTTGGCTCTATT pLKO.1 136 CDS 100% 13.200 9.240 N CHST4 n/a
5 TRCN0000434631 TTTCCACAGAGATGCAAATTC pLKO_005 1650 3UTR 100% 13.200 9.240 N CHST4 n/a
6 TRCN0000034834 GCTGGTCTTTGCCCTATGAAA pLKO.1 1092 CDS 100% 5.625 3.938 N CHST4 n/a
7 TRCN0000034835 TGTGACATCATCCCACAAGAT pLKO.1 500 CDS 100% 4.950 3.465 N CHST4 n/a
8 TRCN0000034838 TGCCATGAATTTGCTGGGCTA pLKO.1 1144 CDS 100% 2.160 1.512 N CHST4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001166395.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11462 pDONR223 100% 95.8% 95.8% None 1_48del n/a
2 ccsbBroad304_11462 pLX_304 0% 95.8% 95.8% V5 1_48del n/a
3 TRCN0000479935 CTAACCTAAAAATTACGGTACTTT pLX_317 39.2% 95.8% 95.8% V5 1_48del n/a
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