Construct: ORF TRCN0000479943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003831.1_s317c1
- Derived from:
- ccsbBroadEn_09735
- DNA Barcode:
- AACCATTGGACCTGATCTCATGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM229B (161145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479943
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_001348542.2 | 99.8% | 100% | 24G>A |
| 2 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_001348543.2 | 99.8% | 100% | 24G>A |
| 3 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_001348544.2 | 99.8% | 100% | 24G>A |
| 4 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_001348546.1 | 99.8% | 100% | 24G>A |
| 5 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_001348547.2 | 99.8% | 100% | 24G>A |
| 6 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_001348548.2 | 99.8% | 100% | 24G>A |
| 7 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_001348549.2 | 99.8% | 100% | 24G>A |
| 8 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_182526.2 | 99.8% | 100% | 24G>A |
| 9 | human | 161145 | TMEM229B | transmembrane protein 229B | NM_001348541.2 | 78.9% | 79.1% | 1_132del;156G>A |
| 10 | mouse | 268567 | Tmem229b | transmembrane protein 229B | NM_001170401.1 | 90.2% | 95.8% | (many diffs) |
| 11 | mouse | 268567 | Tmem229b | transmembrane protein 229B | NM_178745.4 | 90.2% | 95.8% | (many diffs) |
| 12 | mouse | 268567 | Tmem229b | transmembrane protein 229B | XM_006515909.2 | 90.2% | 95.8% | (many diffs) |
| 13 | mouse | 268567 | Tmem229b | transmembrane protein 229B | XM_006515910.3 | 90.2% | 95.8% | (many diffs) |
| 14 | mouse | 268567 | Tmem229b | transmembrane protein 229B | XM_006515912.3 | 90.2% | 95.8% | (many diffs) |
| 15 | mouse | 268567 | Tmem229b | transmembrane protein 229B | XM_006515913.1 | 90.2% | 95.8% | (many diffs) |
| 16 | mouse | 268567 | Tmem229b | transmembrane protein 229B | XM_006515914.1 | 90.2% | 95.8% | (many diffs) |
| 17 | mouse | 268567 | Tmem229b | transmembrane protein 229B | XM_006515915.3 | 90.2% | 95.8% | (many diffs) |
| 18 | mouse | 268567 | Tmem229b | transmembrane protein 229B | XM_011244117.2 | 90.2% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 570
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtctgcc gagcccctga cagcgctgtc ccgctggtac ctgtatgcca 121 tccacggcta cttctgcgag gtgatgttca cagcggcctg ggagttcgtg gtgaacttga 181 actggaagtt ccctggggtc acgagcgtgt gggccctctt catctacggc acctccatcc 241 tcatcgtgga gcgcatgtac cTGCGGCTGC GCGGCCGCTG CCCGCTGCTC CTGCGCTGCC 301 TCATCTACAC GCTCTGGACC TACCTGTGGG AGTTCACCAC CGGCTTCATC CTGCGCCAGT 361 TCAACGCCTG CCCCTGGGAC TACTCCCAGT TCGACTTTGA CTTCATGGGC CTCATCACCC 421 TGGAGTACGC CGTGCCCTGG TTCTGCGGGG CCCTCATCAT GGAGCAGTTC ATCATCCGCA 481 ACACCCTCCG CCTCCGCTTC GACAAGGACG CTGAGCCCGG GGAGCCCAGC GGCGCCCTAG 541 CCCTGGCCAA CGGCCATGTC AAGACTGACT TGCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACCA 721 TTGGACCTGA TCTCATGTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt