Construct: ORF TRCN0000479953
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009677.1_s317c1
- Derived from:
- ccsbBroadEn_10965
- DNA Barcode:
- ACCTTGCCGGTCCCAGTAATTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEOX2 (4223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479953
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4223 | MEOX2 | mesenchyme homeobox 2 | NM_005924.5 | 99.6% | 99.6% | 202_204delCAC |
| 2 | mouse | 17286 | Meox2 | mesenchyme homeobox 2 | NM_008584.4 | 89.5% | 98% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 975
- ORF length:
- 909
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acacccgctc tttggctgcc tgcgcagccc tcacgccacg gcgcaaggct 121 tgcacccgtt ctcccaatcc tctctcgccc tccatggaag atctgaccat atgtcttacc 181 ccgagctctc tacttcttcc tcatcttgca taatcgcggg ataccccaac gaagagggca 241 tgtttgccag ccagcatcac agggggcacc accaccacca ccaccaccac catcaccacc 301 atcagcagca gcagcaccag gctctgcaaa ccaactggca cctcccgcag atgtcttccc 361 caccgagtgc ggctcggcac agcctctgcc tccagcccga ctctggaggg cccccagagt 421 tggggagcag cccgcccgtc ctgtgctcca actcttccag cttgggctcc agcaccccga 481 ctggggccgc gtgcgcgccg ggggactacg gccgccaggc actgtcacct gcggaggcgg 541 agaagcgaag cggcggcaag aggaaaagcg acagcTCAGA CTCCCAGGAA GGAAATTACA 601 AGTCAGAAGT CAACAGCAAA CCCAGGAAAG AAAGGACAGC ATTTACCAAA GAGCAAATCA 661 GAGAACTTGA AGCAGAATTT GCCCATCATA ATTATCTCAC CAGACTGAGG CGATACGAGA 721 TAGCAGTGAA TCTGGATCTC ACTGAAAGAC AGGTGAAAGT CTGGTTCCAA AACAGGCGGA 781 TGAAGTGGAA GAGGGTAAAG GGTGGACAGC AAGGAGCTGC GGCTCGGGAA AAGGAACTGG 841 TGAATGTGAA AAAGGGAACA CTTCTCCCAT CAGAGCTGTC GGGAATTGGT GCAGCCACCC 901 TCCAGCAAAC AGGGGACTCT ATAGCAAATG AAGACAGTCA CGACAGTGAC CACAGCTCAG 961 AGCATGCGCA CTTATGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACCTTGCCGG TCCCAGTAAT 1141 TCAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt